Results 121 to 130 of about 26,375 (226)

Direct measurement of DNA-mediated adhesion between lipid bilayers

, 2015
Multivalent interactions between deformable mesoscopic units are ubiquitous in biology, where membrane macromolecules mediate the interactions between neighbouring living cells and between cells and solid substrates.
Cicuta, P., Di Michele, L., Mognetti, B. M., Orsi, D., Parolini, L., Shimobayashi, S. F.   +5 more
core   +1 more source

Forming and loading giant unilamellar vesicles with acoustic jetting

Biomicrofluidics, 2020
Giant unilamellar vesicles (GUVs) are a useful platform for reconstituting and studying membrane-bound biological systems, offering reduced complexity compared to living cells. Several techniques exist to form GUVs and populate them with biomolecules of interest.
Maxim Armstrong, Michael D. Vahey, Thomas P. Hunt, Daniel A. Fletcher   +3 more
openaire   +3 more sources

Charged giant unilamellar vesicles prepared by electroformation exhibit nanotubes and transbilayer lipid asymmetry

Scientific Reports, 2018
Giant unilamellar vesicles (GUVs) are increasingly used as a versatile research tool to investigate membrane structure, morphology and phase state. In these studies, GUV preparation is typically enhanced by an externally applied electric field, a process
Jan Steinkühler, Philippe De Tillieux, Roland L. Knorr, R. Lipowsky, R. Dimova   +4 more
semanticscholar   +1 more source

Electroformation in a flow chamber with solution exchange as a means of preparation of flaccid giant vesicles

, 2008
A recently described technique (Estes and Mayer, Biochim. Biophys. Acta 1712 (2005) 152--160) for the preparation of giant unilamellar vesicles (GUVs) in solutions with high ionic strength is examined.
Akashi, Angelova, Angelova, Angelova, Angelova, Bagatolli, Bloom, Božič, Bresseleers, Deamer, Dimova, D’Errico, Estes, Estes, Estes, Evans, Heinrich, Hope, Koslov, Kralj-Iglič, Kuribayashi, Mally, Mally, Mathivet, Morse, Moscho, Needham, Okumura, Olbrich, Orbach, Paula, Paula, Primož Peterlin, Redelmeier, Reeves, Riske, Rodriguez, Taylor, Ternström, Vesna Arrigler, Yamashita   +40 more
core   +1 more source

A novel minimal in vitro system for analyzing HIV-1 Gag mediated budding

, 2014
A biomimetic minimalist model membrane was used to study the mechanism and kinetics of cell-free in vitro HIV-1 Gag budding from a giant unilamellar vesicle (GUV).
Gill, Sarjeet, Gui, Dong, Gupta, Sharad, Huang, I-Chueh, Mohideen, Umar, Rao, A. L. N., Xu, Jun, Zandi, Roya   +7 more
core   +1 more source

Induced phagocytic particle uptake into a giant unilamellar vesicle

Soft Matter, 2014
Phagocytosis, the uptake and ingestion of solid particles into living cells, is a central mechanism of our immune system. Due to the complexity of the uptake mechanism, the different forces involved in this process are only partly understood. Therefore the usage of a giant unilamellar vesicle (GUV) as the simplest biomimetic model for a cell allows one
Meinel, Andreas, Tränkle, Benjamin, Römer, Winfried, Rohrbach, Alexander   +3 more
openaire   +4 more sources

Dynamic and Sequential Protein Reconstitution on Negatively Curved Membranes by Giant Vesicles Fusion

Bio-Protocol, 2019
In vitro investigation of the interaction between proteins and positively curved membranes can be performed using a classic nanotube pulling method.
Nicola Franceschi, Maryam Alqabandi, Winfried Weissenhorn, Patricia Bassereau   +3 more
doaj   +1 more source

Polyelectrolyte-induced peeling of charged multilamellar vesicles

, 2005
We study mixtures of charged surfactants, which alone in solution form uni- and multilamellar vesicles, and oppositely charged polyelectrolytes (PEs).
Ramos, Laurence, Vivares, Edith
core  

Determination of biomembrane bending moduli in fully atomistic simulations. [PDF]

, 2014
The bilayer bending modulus (Kc) is one of the most important physical constants characterizing lipid membranes, but precisely measuring it is a challenge, both experimentally and computationally.
Brown, Frank LH, Lerner, Michael G, Levine, Zachary A, Pastor, Richard W, Shea, Joan-Emma, Venable, Richard M, Watson, Max C   +6 more
core  

Solid domains in lipid vesicles and scars

, 2005
The free energy of a crystalline domain coexisting with a liquid phase on a spherical vesicle may be approximated by an elastic or stretching energy and a line tension term.
A Travesset, Alberts, Feigenson G. W., Gordon V., Lipowsky R., Scherfeld D., Witten T. A., Y Chushak   +7 more
core   +2 more sources