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Intravesicular Solute Delivery and Surface Area Regulation in Giant Unilamellar Vesicles Driven by Cycles of Osmotic Stresses. [PDF]
Sambre PD, Ho JCS, Parikh AN.
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Artificial cells with viscoadaptive behavior based on hydrogel-loaded giant unilamellar vesicles. [PDF]
Llopis-Lorente A +5 more
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Protein Reconstitution Inside Giant Unilamellar Vesicles
Annual Review of Biophysics, 2021Giant unilamellar vesicles (GUVs) have gained great popularity as mimicries for cellular membranes. As their sizes are comfortably above the optical resolution limit, and their lipid composition is easily controlled, they are ideal for quantitative light microscopic investigation of dynamic processes in and on membranes.
Litschel, T., Schwille, P.
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Microdomain evolution on giant unilamellar vesicles
Biomechanics and Modeling in Mechanobiology, 2012A chemo-mechanical model is used to capture the formation and evolution of microdomains on the deforming surface of giant unilamellar vesicles. The model is intended for the regime of vesicle dynamics characterized by a distinct difference in time scales between shape change and species transport.
Anand, Embar, John, Dolbow, Eliot, Fried
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Starch aided synthesis of giant unilamellar vesicles
Chemistry and Physics of Lipids, 2020Synthesis of giant unilamellar vesicles (GUVs) of charged and uncharged lipids at physiological salt concentration is presented using the starch hydrogel method as an example of the gel assisted synthesis method. The swelling of the gel is assisted by the presence of a high amount of amylopectin in starch and yields giant-sized vesicles, which are ...
Mohammad, Maoyafikuddin +2 more
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Cheap portable electroformed giant unilamellar vesicles preparation kit
Journal of Liposome Research, 2022The membrane of a cell separates the internal and external media of the cell and contributes to a variety of important processes, including gradient maintenance and signal transduction. Synthetic lipid-made vesicles are commonly utilized as cell membrane model systems.
Zendeh, Zahra +2 more
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Refined contour analysis of giant unilamellar vesicles
The European Physical Journal E, 2004The fluctuation spectrum of giant unilamellar vesicles is measured using a high-resolution contour detection technique. An analysis at higher q vectors than previously achievable is now possible due to technical improvements of the experimental setup and of the detection algorithm.
Pécréaux, J. +4 more
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Phase Transition Induced Adhesion of Giant Unilamellar Vesicles
ChemPhysChem, 2009AbstractCell and vesicle adhesion is believed to be dictated by the balance between a local interaction potential, which represents the sum of all attractive and repulsive forces and the elastic energy. Changing the mechanical properties of the membrane therefore offers a sensitive tool to control vesicle adhesion.
Frank, T. +3 more
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Methods for Forming Giant Unilamellar Fatty Acid Vesicles
2021Fatty acids readily assemble into bilayer membranes at a pH near their apparent pKa. Fatty acid vesicles are not only useful for research in the fields of origins of life, soft matter science, biophysics, and drug delivery, but are also cost-effective and easy to manipulate, making them ideal for teaching students about self-assembly and lipid bilayers.
Lauren A, Lowe +2 more
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Giant unilamellar vesicle formation under physiologically relevant conditions
Chemistry and Physics of Lipids, 2008We present an upgrade to the giant unilamellar vesicle (GUV) electroformation method allowing easy GUV production in different buffers and with various membrane compositions. Our experimental results reveal that lipid deposits obtained from aqueous liposome or proteoliposome dispersions are highly efficient for GUV electroformation.
Pott, T. +2 more
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