Formation of Giant Unilamellar Vesicles Assisted by Fluorinated Nanoparticles. [PDF]
Adv Sci (Weinh), 2023 In the quest to produce artificial cells, one key challenge that remains to be solved is the recreation of a complex cellular membrane. Among the existing models, giant unilamellar vesicles (GUVs) are particularly interesting due to their intrinsic ...
Waeterschoot J +5 more
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Functional reconstitution of plant plasma membrane H<sup>+</sup>-ATPase into giant unilamellar vesicles. [PDF]
Sci RepMembrane transporters are essential for numerous biological processes by controlling the movement of ions and molecules across cell membranes. However, dissecting their molecular dynamics in complex cellular environments presents significant challenges ...
Uzun HD +3 more
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Low-cost equipment for electroformation of Giant Unilamellar Vesicles [PDF]
HardwareX, 2018 Giant Unilamellar Vesicles (GUVs) constitute a very useful model for biomembranes. They are usually generated by electroformation using sinusoidal potential waves applied between two platinum electrodes or electrodes composed of glasses covered by indium
Javier Alejandro Bellon +2 more
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Osmotic Gradients Induce Bio-reminiscent Morphological Transformations in Giant Unilamellar Vesicles [PDF]
Frontiers in Physiology, 2012 We report observations of large-scale, in-plane and out-of-plane membrane deformations in giant uni- and multilamellar vesicles composed of binary and ternary lipid mixtures in the presence of net transvesicular osmotic gradients.
Kamila eOglecka +4 more
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Effect of the Membrane Composition of Giant Unilamellar Vesicles on Their Budding Probability: A Trade-Off between Elasticity and Preferred Area Difference [PDF]
Life, 2021 The budding and division of artificial cells engineered from vesicles and droplets have gained much attention in the past few decades due to an increased interest in designing stimuli-responsive synthetic systems.
Ylenia Miele +3 more
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Functional reconstitution of a voltage-gated potassium channel in giant unilamellar vesicles. [PDF]
PLoS ONE, 2011 Voltage-gated ion channels are key players in cellular excitability. Recent studies suggest that their behavior can depend strongly on the membrane lipid composition and physical state. In vivo studies of membrane/channel and channel/channel interactions
Sophie Aimon +5 more
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Light-Switchable Membrane Permeability in Giant Unilamellar Vesicles. [PDF]
Pharmaceutics, 2022 In this work, giant unilamellar vesicles (GUVs) were synthesized by blending the natural phospholipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) with a photoswitchable amphiphile (1) that undergoes photoisomerization upon irradiation with UV-
Albanese P +6 more
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A Fluorescence-based Assay for Measuring Phospholipid Scramblase Activity in Giant Unilamellar Vesicles. [PDF]
Bio Protoc, 2022 Transbilayer movement of phospholipids in biological membranes is mediated by a diverse set of lipid transporters. Among them are scramblases that facilitate rapid bi-directional movement of lipids without metabolic energy input.
Mathiassen PPM, Pomorski TG.
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Rapid Encapsulation of Reconstituted Cytoskeleton Inside Giant Unilamellar Vesicles. [PDF]
J Vis Exp, 2021 Bashirzadeh Y +4 more
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Curcumin-Induced Stabilization of Protein-Based Nano-Delivery Vehicles Reduces Disruption of Zwitterionic Giant Unilamellar Vesicles. [PDF]
Molecules, 2022 Curcumin-loaded native and succinylated pea protein nanoparticles, as well as zwitterionic giant unilamellar vesicles were used in this study as model bioactive compound loaded-nanoparticles and biomembranes, respectively, to assess bio-nano interactions.
Okagu OD, Abioye RO, Udenigwe CC.
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