Results 11 to 20 of about 11,661 (108)

Construction of membrane-bound artificial cells using microfluidics: a new frontier in bottom-up synthetic biology [PDF]

open access: yes, 2016
The quest to construct artificial cells from the bottom-up using simple building blocks has received much attention over recent decades and is one of the grand challenges in synthetic biology.
Elani, Y
core   +2 more sources

Visualization of membrane loss during the shrinkage of giant vesicles under electropulsation [PDF]

open access: yes, 2009
We study the effect of permeabilizing electric fields applied to two different types of giant unilamellar vesicles, the first formed from EggPC lipids and the second formed from DOPC lipids.
Abidor   +64 more
core   +6 more sources

Active Membrane Fluctuations Studied by Micropipet Aspiration [PDF]

open access: yes, 2001
We present a detailed analysis of the micropipet experiments recently reported in J-B. Manneville et al., Phys. Rev. Lett. 82, 4356--4359 (1999), including a derivation of the expected behaviour of the membrane tension as a function of the areal strain ...
A. N. Radionov   +52 more
core   +2 more sources

Coupling between pore formation and phase separation in charged lipid membranes [PDF]

open access: yes, 2015
We investigated the effect of charge on the membrane morphology of giant unilamellar vesicles (GUVs) composed of various mixtures containing charged lipids.
Hamada, Tsutomu   +5 more
core   +2 more sources

Transmembrane Activation of Catalysis and Protein Refolding in Synthetic Cells by Enzymes and Nanozymes

open access: yesAdvanced Functional Materials, EarlyView.
Synthetic cells are engineered herein to respond to an external chemical messenger by the activation of intracellular catalysis. The chemical messenger molecules are catalytically generated by an extracellular enzyme or a mineral surface, whereas the intracellular catalysis emerges via direct enzyme activation or via protein refolding.
Dante G. Andersen   +5 more
wiley   +1 more source

Electroformation in a flow chamber with solution exchange as a means of preparation of flaccid giant vesicles

open access: yes, 2008
A recently described technique (Estes and Mayer, Biochim. Biophys. Acta 1712 (2005) 152--160) for the preparation of giant unilamellar vesicles (GUVs) in solutions with high ionic strength is examined.
Akashi   +40 more
core   +1 more source

High‐Resolution Patterned Delivery of Chemical Signals From 3D‐Printed Picoliter Droplet Networks

open access: yesAdvanced Materials, EarlyView.
3D‐printed picoliter droplet networks have been fabricated that control gene expression in bacterial populations by releasing chemical signals with precise spatial definition and high temporal resolution. This system of effector release is widely applicable, offering diverse applications in biology and medicine.
Jorin Riexinger   +7 more
wiley   +1 more source

Mechanism of cellular uptake of genotoxic silica nanoparticles. [PDF]

open access: yes, 2012
Mechanisms for cellular uptake of nanoparticles have important implications for nanoparticulate drug delivery and toxicity. We have explored the mechanism of uptake of amorphous silica nanoparticles of 14 nm diameter, which agglomerate in culture medium ...
Brown, AP   +5 more
core   +3 more sources

A Photonastic Prototissue Capable of Photo‐Mechano‐Chemical Transduction

open access: yesAdvanced Materials, EarlyView.
Mimicking energy transduction in prototissue assemblies remains a challenge of bottom‐up synthetic biology. In this work, prototissues integrating protocells with photothermal gold nanoparticle proto‐organelles and a thermoresponsive polymeric proto‐cortex are developed.
Agostino Galanti   +7 more
wiley   +1 more source

Enhanced and Durable Light‐Driven Hydrogen Evolution by Cobalt‐Based Prussian Blue Analogs in Phospholipid Bilayers

open access: yesAdvanced Energy and Sustainability Research, EarlyView.
Light‐driven H2 evolution catalysis is reported for oleylamine‐capped MCo Prussian blue analogs (PBAs, M = Ni, Co, Zn, Cu) within phospholipid bilayers and using a ruthenium‐based amphiphilic photosensitizer. The hydrophobic interactions between the active units bring them in close proximity facilitating catalysis.
Subrata Mandal   +5 more
wiley   +1 more source

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