PCR cloning Intermediated Gibson assembly (PIG) for Constructing DNA Repair Templates in CRISPR-Cas9 Based Gene Editing. [PDF]
MicroPubl Biol, 2023 Han D, Churcher S, Nordman JT.
europepmc +1 more source
Oral History Interview with Kelly Gibson, May 20, 2005
, 2005 The National Museum of the Pacific War presents an oral interview with Kelly Gibson. Gibson joined the Army Air Corps in September, 1941. He was trained as a radio repairman and serviced radios in airplanes at Turner Field, Georgia.
Gibson, Kelly
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Huntingtin Aggregate‐Responsive Autophagy Gene Circuit Mitigates Disease Pathology in R6/2 Mice
Advanced Science, EarlyView.CD98‐mediated receptor‐mediated transcytosis enables LIP‐CD98 nanocarriers to cross the blood–brain barrier and deliver ARAA to neurons. mHTT aggregates activate the 11G–NarX sensor, initiating Auto‐P and Trans‐P signaling through the VP48–NarL relay.
Jie Zhu +8 more
wiley +1 more source
4G cloning: rapid gene assembly for expression of multisubunit protein complexes in diverse hosts
Life Science AllianceTo streamline expression construct preparation for screening in diverse expression hosts, we present vectors and protocols using Golden Gate ligation to guide one-step Gibson assembly. Multisubunit protein complexes are central to many cellular processes,
Michael Taschner +3 more
doaj +1 more source
“Lithium‐Free” Anode Solid‐State Batteries Enabled by 3D Structured Miec Garnet
Advanced Energy Materials, EarlyView.“Lithium‐free” anode solid‐state batteries can maximize energy density by eliminating excess lithium metal but are limited by unstable lithium nucleation and poor lithium reversibility at solid interfaces. A mixed ionic and electronic conducting garnet scaffold promotes uniform lithium deposition within a three‐dimensional porous framework at low ...
George V. Alexander +6 more
wiley +1 more source
Geophysical Monograph Series, Page 1-26., 2021 Exploring the links between Large Igneous Provinces and dramatic environmental impact
An emerging consensus suggests that Large Igneous Provinces (LIPs) and Silicic LIPs (SLIPs) are a significant driver of dramatic global environmental and biological changes, including mass extinctions.
Richard E. Ernst +8 more
wiley +2 more sources
Effective Gene Silencing in Plants by Synthetic Trans-Acting siRNAs Derived From Minimal Precursors
Bio-ProtocolSynthetic trans-acting small interfering RNAs (syn-tasiRNAs) are 21-nucleotide small RNAs designed to induce highly specific and efficient gene silencing in plants.
Adriana Cisneros +3 more
doaj +1 more source
L‐Cysteine and N‐Acetylcysteine Supplementation Improves Clinical Outcome in a Patient With COXPD10
American Journal of Medical Genetics Part A, EarlyView.ABSTRACT MTO1 is a nuclear gene that encodes a mitochondrial protein essential for modifying mitochondrial transfer RNAs (tRNAs) and stabilizing codon‐anticodon interactions to ensure accurate and efficient mitochondrial protein synthesis and oxidative phosphorylation.
Nishitha R. Pillai +5 more
wiley +1 more source
Construction and Quantitation of a Selectable Protein Splicing Sensor Using Gibson Assembly and Spot Titers. [PDF]
Curr Protoc, 2021 Woods D +3 more
europepmc +1 more source
Establishment of Humanized EFNB2/B3 Murine Models for Investigating Nipah Virus Pathogenesis
Animal Research and One Health, EarlyView.ABSTRACT Nipah virus (NiV) is a zoonotic paramyxovirus classified as a WHO priority pathogen, which causes severe respiratory disease and encephalitis in humans with high mortality. Currently, no licensed vaccines or specific antiviral therapies are available.
Wei Dong +6 more
wiley +1 more source