Results 71 to 80 of about 1,453,402 (230)
Efficient dual-negative selection for bacterial genome editing
BMC Microbiology, 2020 Background Gene editing is key for elucidating gene function. Traditional methods, such as consecutive single-crossovers, have been widely used to modify bacterial genomes.
Francesca Romana Cianfanelli +2 more
doaj +1 more source
Engineered Transformer Base Editor with Enhanced Editing Efficiency
Advanced Science, EarlyView.A highly efficient transformer base editor system achieves robust genomic editing in a humanized mouse model. This work establishes a versatile and translatable platform, opening new avenues for precision gene therapy. ABSTRACT Canonical cytosine base editors (CBEs) achieve precise C‐to‐T conversions without inducing DNA double‐strand breaks (DSBs ...
Bowen Chen +5 more
wiley +1 more source
pTARGEX vectors: a versatile toolbox for plant-based protein expression
Frontiers in Synthetic BiologyThe ability to express heterologous proteins in specific plant tissues and subcellular compartments is critical for advancing plant-based biotechnologies and biopharming research.
Camille Chartrand-Pleau +11 more
doaj +1 more source
The Gibson Paradox: An Empirical Investigation for Turkey [PDF]
This paper tests the existence of Gibson paradox using the traditional and modern time series techniques in the case of annual Turkish data. Even though the results from the traditional Gibson paradox regression suggested a positive relationship between ...
Halicioglu, Ferda
core
Advanced Science, EarlyView.Using genetic code expansion, we engineered vascularized human cerebral organoids (vhCOs) with microglia‐like cells and blood‐brain barrier features. vhCOs recapitulate neurovascular interactions, regional identities, and neuronal subtypes resembling the fetal brain.
Haishuang Lin +7 more
wiley +1 more source
Reversible, Chemically Gated FRET via Ligand‐Activated Acceptors
Advanced Science, EarlyView.Fluorogen binding turns the compact FAST tag into a tunable FRET acceptor, with ligand chemistry programming spectral overlap and excited‐state lifetimes. Fluorescence‐lifetime imaging reads out energy transfer through the shortened donor lifetime, while simply adding or washing out the dye switches FRET on and off, reversibly mapping the supramodular ...
Nivedita Singh +7 more
wiley +1 more source
Advanced Science, EarlyView.An intelligent engineered probiotic platform is developed for the precision management of inflammatory bowel disease. By integrating surface‐displayed adhesins with an inflammation‐responsive CAT‐SOD‐GPx tripartite fusion system and mucosal repair factors, this programmable system achieves prolonged intestinal retention and multi‐pronged therapy.
Yuxi Wang +9 more
wiley +1 more source
Advanced Science, EarlyView.A HER3‐targeted sonogenetic lipid nanoparticle platform is developed for triple‐negative breast cancer therapy. An AI‐designed miniprotein inhibits HER3 signaling, while TRPV4 and NFAT‐responsive IL‐15 plasmids enable ultrasound‐controlled calcium signaling and cytokine expression. The system induces Ca2+ overload, ROS generation, mitochondrial damage,
Junya Song +6 more
wiley +1 more source
Advanced Science, EarlyView.We present a versatile DNA‐encoded library (DEL) platform designed for rapid library synthesis and subsequent screening for chemical inducers of proximity (CIPs) across various presenter proteins of choice. Applying this approach, we discovered a novel molecular glue‐like compound mediating CRBN‐BRD9 ternary complex formation with remarkable ...
Bingqi Tong +11 more
wiley +1 more source
Oral History Interview with Linda Reynolds Gibson, March 30, 2022
, 2022 Recording of an interview with Linda Reynolds Gibson, an educator from Kerrville. Gibson discusses her family, education, teaching positions, her participation in the establishment of a childcare center at Tivy High School for young mothers , and her ...
Jones, Mary Elaine +1 more
core +1 more source