Results 51 to 60 of about 1,453,402 (230)
Nimble Cloning: A Simple, Versatile, and Efficient System for Standardized Molecular Cloning
Frontiers in Bioengineering and Biotechnology, 2020 Molecular cloning is one of the most fundamental technologies in molecular biology, and has been critical for driving biotechnological advances. In this study, we have developed a novel method for standardized molecular cloning.
Pu Yan +5 more
doaj +1 more source
One step generation of customizable gRNA vectors for multiplex CRISPR approaches through string assembly gRNA cloning (STAgR). [PDF]
PLoS ONE, 2018 Novel applications based on the bacterial CRISPR system make genetic, genomic, transcriptional and epigenomic engineering widely accessible for the first time.
Christopher T Breunig +8 more
doaj +1 more source
A Generative Neuro‐Symbolic AI for Protein Sequence Design
Advanced Science, EarlyView.We introduce EffieDes, a neuro‐symbolic framework coupling deep learning‐based fitness landscape parameterization with exact automated reasoning. Unlike greedy sampling, EffieDes identifies sequences that globally optimize fitness while satisfying intricate design constraints.
Marianne Defresne +12 more
wiley +1 more source
OVERFRAG: An overlapping DNA fragments generator for molecular cloning and synthetic biology
Data in Brief, 2019 DNA synthesis and homologous recombination can be used to simplify molecular cloning and to make synthetic biology easily accessible (M.J. Czar et al., 2009). However, the design of overlapping DNA fragments to construct large molecules is time-consuming
Jose Ribamar Ferreira-Junior +1 more
doaj +1 more source
Advanced Science, EarlyView.Engineering of the 148‐germline (148‐GL) antibody reveals a roadmap for overcoming SARS‐CoV‐2 viral escape. By introducing four specific somatic hypermutations, the resulting 148‐M4 variant restores potent neutralization against Omicron BA.1 and BA.4/5.
Huibin Lv +14 more
wiley +1 more source
Repository-based plasmid design.
PLoS ONE, 2020 There was an explosion in the amount of commercially available DNA in sequence repositories over the last decade. The number of such plasmids increased from 12,000 to over 300,000 among three of the largest repositories: iGEM, Addgene, and DNASU.
Joshua J Timmons, Doug Densmore
doaj +1 more source
A simplified and versatile system for the simultaneous expression of multiple siRNAs in mammalian cells using Gibson DNA Assembly. [PDF]
PLoS ONE, 2014 RNA interference (RNAi) denotes sequence-specific mRNA degradation induced by short interfering double-stranded RNA (siRNA) and has become a revolutionary tool for functional annotation of mammalian genes, as well as for development of novel therapeutics.
Fang Deng +23 more
doaj +1 more source
Therapeutic Gene Editing of APOE4 in Sporadic Alzheimer's Disease via Prime Editor 7
Advanced Science, EarlyView.Prime Editor 7‐mediated conversion of APOE4 to APOE3 alleviates Alzheimer's disease‐associated pathology in AD mouse models and patient‐derived neurons and improves cognitive performance in vivo, supporting therapeutic genome editing as a promising strategy for APOE4‐associated neurodegeneration.
Yunkyung Kim +16 more
wiley +1 more source
Advanced Science, EarlyView.A programmable encapsulation technology is developed to reduce bacterial immunogenicity and proliferation after systemic administration. The cross‐linked polymer networks around individual bacteria enable immunogenic shielding and permit selective proliferation in tumors, allowing the bacteria to convert tumor‐accumulated ammonia into L‐arginine and ...
Jianhui Yang +12 more
wiley +1 more source
, 1975 From Sketches and Cartoons. A Gibson Girl in the full hairstyle of 1898. To cover the forehead, it is drawn to one side.
Gibson, Charles Dana, 1867-1944 +1 more
core +1 more source