Results 31 to 40 of about 1,453,402 (230)
Mrs. M. E. Gibson, envelope addressed to Mrs. J. S. Gibson, postmarked December 4, 1943
, 2023 This envelope contained a letter sent from Mrs. M. E. Gibson, Box 7, Tuskegee Institute, now known as Tuskegee University, Alabama. It was addressed to Mrs. J. S.
Gibson, Maggie Eleanor Chiles, 1869-1944
core
A rapid seamless method for gene knockout in Pseudomonas aeruginosa
BMC Microbiology, 2017 Background Pseudomonas aeruginosa is a model organism for the study of quorum sensing, biofilm formation, and also leading cause of nosocomial infections in immune compromised patients. As such P.
Weiliang Huang, Angela Wilks
doaj +1 more source
ADP‐ribosylation: An emerging regulator of the epigenome
Molecular Oncology, EarlyView.ADP‐ribosylation has emerged as a dynamic epigenetic signaling mechanism that modifies histones and chromatin‐associated proteins. Through coordinated PARylation and MARylation, it integrates with other histone modifications to regulate chromatin structure, transcription factor activity, and gene expression, influencing genome function and disease ...
Cristel V. Camacho +2 more
wiley +1 more source
CRISPR/Cas9 nuclease cleavage combined with Gibson assembly for seamless cloning
BioTechniques, 2015 Restriction enzymes have two major limitations for cloning: they cannot cleave at any desired location in a DNA sequence and may not cleave uniquely within a DNA sequence. In contrast, the clustered regularly interspaced short palindromic repeat (CRISPR)–
Jia-Wang Wang +6 more
doaj +1 more source
Gibson Assembly® Master Mix – Assembly (E2611) v1
, 2014 This is the protocol for the Gibson Assembly using the Gibson Assembly® Master Mix (E2611).
core +1 more source
DNA assembly with error correction on a droplet digital microfluidics platform
BMC Biotechnology, 2018 Background Custom synthesized DNA is in high demand for synthetic biology applications. However, current technologies to produce these sequences using assembly from DNA oligonucleotides are costly and labor-intensive.
Yuliya Khilko +5 more
doaj +1 more source
MARK4 enhances stress granule formation under oxidative stress and increases tau accumulation
FEBS Open Bio, EarlyView.MARK4 (red dots) localizes to stress granules (orange dots) and promotes their formation under oxidative stress by modulating TIA1 (blue dots). MARK4 and TIA1 synergistically increase tau (purple) accumulation, and the reduction of the TIA1 ortholog suppresses neurodegeneration in a fly model.
Sho Nakajima +8 more
wiley +1 more source
Вавиловский журнал генетики и селекции, 2019 The discovery of the position effect variegation phenomenon and the subsequent comprehensive analysis of its molecular mechanisms led to understanding that the local chromatin composition has a dramatic effect on gene activity.
M. O. Lebedev +3 more
doaj +1 more source
Plant X-tender: An extension of the AssemblX system for the assembly and expression of multigene constructs in plants. [PDF]
PLoS ONE, 2018 Cloning multiple DNA fragments for delivery of several genes of interest into the plant genome is one of the main technological challenges in plant synthetic biology.
Tjaša Lukan +5 more
doaj +1 more source
PCR and Gibson assembly using cellular reagents.
, 2018 (a) Schematic depicting cellular PCR followed by cellular Gibson assembly for constructing new plasmids. Bacteria harboring target plasmids are mixed with polymerase-expressing cellular reagents and PCR is initiated by adding appropriate primers, buffer,
Arti Pothukuchy (2693182) +7 more
core +1 more source