Results 21 to 30 of about 1,453,402 (230)
Versatile vector tools for efficient protein screening across multiple expression systems. [PDF]
FEBS Open BioA unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhu Z +5 more
europepmc +2 more sources
User-Friendly Reverse Genetics System for Modification of the Right End of Fowl Adenovirus 4 Genome
Viruses, 2020 A novel fowl adenovirus 4 (FAdV-4) has caused significant economic losses to the poultry industry in China since 2015. We established an easy-to-use reverse genetics system for modification of the whole right and partial left ends of the novel FAdV-4 ...
Bingyu Yan +8 more
doaj +1 more source
Antibiotics, 2020 Humidimycin (MDN-0010) is a ribosomally synthesized and post-translationally modified peptide (RiPP) belonging to class I lasso peptides, and is structurally related to siamycins, which have been shown to have strong antimicrobial activities against Gram-
Marina Sánchez-Hidalgo +2 more
doaj +1 more source
Nature Communications, 2020 Several different genetic strategies have been reported for the modification of polyketide synthases but the highly repetitive modular structure makes this difficult.
Kei Kudo +10 more
doaj +1 more source
BioTechniques, 2015 Techniques such as Gibson and Golden Gate assembly and homologous recombination in yeast allow researchers to assemble DNA sequences millions of base pairs in length. Sarah Webb investigates the challenges of constructing designer chromosomes and genomes,
Sarah Webb
doaj +1 more source
SmartBac, a new baculovirus system for large protein complex production
Journal of Structural Biology: X, 2019 Recent revolution of cryo-electron microscopy has opened a new door to solve high-resolution structures of macromolecule complexes without crystallization while how to efficiently obtain homogenous macromolecule complex sample is therefore becoming a ...
Yujia Zhai +4 more
doaj +1 more source
Generation and characterization of UL41 null pseudorabies virus variant in vitro and in vivo
Virology Journal, 2018 Background The alphaherpesvirus virion host shutoff (vhs) gene, UL41, can induce degradation of host mRNAs and shut off host protein synthesis. The roles of vhs in HSV-1 and HSV-2 have been studied extensively in previous studies, however, relatively ...
Chao Ye +11 more
doaj +1 more source
Cellular reagents for diagnostics and synthetic biology. [PDF]
PLoS ONE, 2018 We have found that the overproduction of enzymes in bacteria followed by their lyophilization leads to 'cellular reagents' that can be directly used to carry out numerous molecular biology reactions.
Sanchita Bhadra +7 more
doaj +1 more source
GAMER-Ad: a novel and rapid method for generating recombinant adenoviruses
Molecular Therapy: Methods & Clinical Development, 2021 Oncolytic adenoviruses have become ideal agents in the path toward treating cancer. Such viruses have been engineered to conditionally replicate in malignant cells in which certain signaling pathways have been disrupted.
Firas Hamdan +10 more
doaj +1 more source
MicrobiologyOpen, 2020 Most DNA assembly methods require bacterial amplification steps, which restrict its application to genes that can be cloned in the bacterial host without significant toxic effects. However, genes that cannot be cloned in bacteria do not necessarily exert
Vicente F. Cataldo +3 more
doaj +1 more source