Generation of full-length wild-type and mutated futsch transgenes in Drosophila—efficient Gibson Assembly of ultra-large cDNAs [PDF]
FlyDrosophila Futsch is a key microtubule-associated protein (fly homolog of MAP1B) that regulates microtubule organization, synaptic terminal growth, and neuronal development.
Chunlai Wu
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An improved toolkit of gateway- and gibson assembly-compatible vectors for protoplast transfection and agrobacterium-mediated plant transformation [PDF]
BMC Research NotesObjective Understanding the regulation and function of plant genes is essential for addressing the challenges faced by modern agriculture. Plant transformation, in conjunction with fluorescence microscopy, offers a powerful approach to investigate the ...
Jeffrey R. Allen +5 more
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Update Notice: Efficient Generation of Genome-wide Libraries for Protein–ligand Screens Using Gibson Assembly [PDF]
Bio-Protocol, 2023 Tamara Sternlieb +3 more
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Rapid and Effective Generation of Nanobody Based CARs using PCR and Gibson Assembly [PDF]
International Journal of Molecular Sciences, 2020 Joline Ingels +2 more
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Modular plug-and-play engineering of Klebsiella phages with dual receptor-binding proteins for efficient host range design [PDF]
Journal of Biological EngineeringBackground The high capsular diversity restricting the host range of many Klebsiella phages has driven the evolution of branched receptor-binding protein (RBP) systems as a strategy for host range expansion.
Dorien Dams +4 more
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Seamless Insert-Plasmid Assembly at High Efficiency and Low Cost. [PDF]
PLoS ONE, 2016 Seamless cloning methods, such as co-transformation cloning, sequence- and ligation-independent cloning (SLIC) or the Gibson assembly, are essential tools for the precise construction of plasmids.
Roger M Benoit +5 more
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Restriction-Assembly: A Solution to Construct Novel Adenovirus Vector
Viruses, 2022 Gene therapy and vaccine development need more novel adenovirus vectors. Here, we attempt to provide strategies to construct adenovirus vectors based on restriction-assembly for researchers with little experience in this field.
Xiaojuan Guo +7 more
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Rational Design of High-Number dsDNA Fragments Based on Thermodynamics for the Construction of Full-Length Genes in a Single Reaction. [PDF]
PLoS ONE, 2015 Gene synthesis is frequently used in modern molecular biology research either to create novel genes or to obtain natural genes when the synthesis approach is more flexible and reliable than cloning.
Bhagyashree S Birla, Hui-Hsien Chou
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Complete Genome Sequencing and Infectious cDNA Clone Construction of Soybean Mosaic Virus Isolated from Shanxi [PDF]
The Plant Pathology Journal, 2021 Soybean mosaic virus (SMV) is the predominant viral pathogen that affects the yield and quality of soybean. The natural host range for SMV is very narrow, and generally limited to Leguminosae.
Defu Wang +4 more
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ApE, A Plasmid Editor: A Freely Available DNA Manipulation and Visualization Program
Frontiers in Bioinformatics, 2022 A plasmid Editor (ApE) is a free, multi-platform application for visualizing, designing, and presenting biologically relevant DNA sequences. ApE provides a flexible framework for annotating a sequence manually or using a user-defined library of features.
M. Wayne Davis, Erik M. Jorgensen
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