Rapid Construction of a Replication-Competent Infectious Clone of Human Adenovirus Type 14 by Gibson Assembly [PDF]
Viruses, 2018 In 1955, Human adenovirus type 14 (HAdV-B14p) was firstly identified in a military trainee diagnosed as acute respiratory disease (ARD) in the Netherlands. Fifty years later, a genomic variant, HAdV-B14p1, re-emerged in the U.S.
Haibin Pan +13 more
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The Application of the Gibson Assembly Method in the Production of Two pKLS3 Vector-Derived Infectious Clones of Foot-and-Mouth Disease Virus [PDF]
Vaccines, 2023 The construction of a full-length infectious clone, essential for molecular virological study and vaccine development, is quite a challenge for viruses with long genomes or possessing complex nucleotide sequence structures.
Ploypailin Semkum +5 more
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A Practical Guide for Introducing Gibson Assembly® Gene Constructs into Chemically Competent Cells
BioTechniques, 2015 The Gibson Assembly® method is an easy-to-use, robust, seamless cloning method that allows for the efficient cloning of multiple DNA fragments simultaneously. Gene constructs assembled with Gibson Assembly® are often introduced into E.
Christine Chen
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Pyramiding stacking of multigenes (PSM): a simple, flexible and efficient multigene stacking system based on Gibson assembly and gateway cloning [PDF]
Frontiers in Bioengineering and Biotechnology, 2023 Genetic engineering of complex metabolic pathways and multiple traits often requires the introduction of multiple genes. The construction of plasmids carrying multiple DNA fragments plays a vital role in these processes.
Dongdong Zeng +7 more
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A simplified Gibson assembly method for site directed mutagenesis by re-use of standard, and entirely complementary, mutagenesis primers [PDF]
BMC Biotechnology, 2022 Background Site-directed mutagenesis (SDM) is a key method in molecular biology; allowing to modify DNA sequences at single base pair resolution. Although many SDM methods have been developed, methods that increase efficiency and versatility of this ...
Shunit Olszakier, Shai Berlin
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Efficient Generation of Genome-wide Libraries for Protein–ligand Screens Using Gibson Assembly
Bio-Protocol, 2022 Genome-wide screens using yeast or phage displays are powerful tools for identifying protein–ligand interactions, including drug or vaccine targets, ligand receptors, or protein–protein interactions.
Tamara Sternlieb +3 more
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Home-made enzymatic premix and Illumina sequencing allow for one-step Gibson assembly and verification of virus infectious clones [PDF]
Phytopathology Research, 2020 An unprecedented number of viruses have been discovered by leveraging advances in high-throughput sequencing. Infectious clone technology is a universal approach that facilitates the study of biology and role in disease of viruses.
Mingmin Zhao +6 more
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In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly [PDF]
STAR Protocols, 2022 Summary: The synthesis of single-stranded riboprobes or double-stranded RNAs for in situ hybridization and gene knockdowns often use vectors that require time-consuming plasmid restriction digests and inefficient gel purifications.
Andrew Wolff +3 more
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BioTechniques, 2023 For difficult overlap extension PCR, a Gibson assembly process was inserted between the two PCR rounds to facilitate the formation of complete gene templates at a moderate temperature.
Junyi Liu +6 more
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A one-step construction of adenovirus (OSCA) system using the Gibson DNA Assembly technology
Molecular Therapy: Oncolytics, 2021 Adenovirus (Ad) is a non-enveloped linear double-stranded DNA virus with >50 serotypes in humans. Ad vectors have been used as gene delivery vehicles to express transgenes, small interfering RNAs (siRNAs) for gene silencing, or CRISPR/Cas and designer ...
Na Ni +22 more
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