Results 61 to 70 of about 1,453,402 (230)
Escherichia coli flagellar genes as target sites for integration and expression of genetic circuits. [PDF]
PLoS ONE, 2014 E. coli is a model platform for engineering microbes, so genetic circuit design and analysis will be greatly facilitated by simple and effective approaches to introduce genetic constructs into the E. coli chromosome at well-characterised loci.
Mario Juhas +6 more
doaj +1 more source
Advanced Science, EarlyView.This study reveals that Tex10 drives oxaliplatin resistance in colorectal cancer by competitively binding BRD9 to disrupt the ncBAF complex, thereby suppressing AMBRA1 transcription and ULK1‐mediated autophagy. Gemcitabine is identified as a direct Tex10 inhibitor that restores autophagy and overcomes resistance.
Ping Xu +9 more
wiley +1 more source
Journal of VirologyThe size and complexity of large viral genomes limit the technical possibilities for genome manipulations in fundamental research and medical or technological applications. State-of-the-art recombineering in bacteria has partially overcome this limit but
Jean-Baptiste Vergnes +3 more
doaj +1 more source
F‐Type Pyocin Versus Phage λ Tail: Conserved Hub, Divergent Fibers
Advanced Science, EarlyView.Near‐atomic cryo‐EM structures of a Gram‐negative F‐type pyocin reveal a conserved tail tip architecture shared with bacteriophage λ together with a previously unrecognized side‐fiber attachment mode. These findings provide new insight into tailocin assembly and host recognition and establish a structural framework for engineering precision ...
Zhiwei Gu +5 more
wiley +1 more source
, 1947 Cover titles vary: Manufacture, service, distribution (masthead, Oct. 1947) -- Service (Feb. 1948-Feb. 1959) -- Foy's news service of the month (v.1, no. 8, Nov. 1959; v. 2, no., Feb. 1960-v. 7, no. 5, June, 1965) -- Cox Bros newsletter (no.
Foy & Gibson
core
RSF1‐Dependent PAR Turnover Promotes 53BP1 Liquid Condensate Formation at DNA Damage Sites
Advanced Science, EarlyView.At sites of DNA damage, RSF1 recruits PARG to accelerate PAR turnover, triggering a switch from PAR‐driven condensates to 53BP1 condensates. This condensate transition enables p53‐dependent gene transcription and coordinates the DNA damage response.
Yungyeong Heo +10 more
wiley +1 more source
Advanced Science, EarlyView.CDegSR, a new tool for imaging RNA at single molecule level in living cells was developed by researchers. Background noise was eliminated by protein engineering and making it degrade when not bound to its target. This allowed individual RNA molecules to be clearly imaged and tracked.
Shipeng Shao, Hongchen Zhang
wiley +1 more source
Advanced Science, EarlyView.Phase separation of SF3B1 acts as a key regulatory mechanism for dynamic alternative splicing throughout early mouse embryogenesis. Its absence triggers extensive splicing errors, which induce persistent DNA damage, defective cell cycle progression, and failed cell lineage commitment, and ultimately hinder the normal growth and development of ...
Kang Zhao +15 more
wiley +1 more source
Advanced Science, EarlyView.Flux‐protection engineering stabilizes an oxidation‐prone L‐DOPA node in engineered E. coli by combining acid‐adapted chassis evolution, respiratory‐chain modulation, enzyme‐level flux matching, and pH–DO control. Carbon is redirected from oxidative browning to productive biosynthesis, enabling 1075.2 mg/L curcumin and demonstrating transferability to ...
Jianbin Chen +10 more
wiley +1 more source
Polyproline: A Synthetic Polypeptide With Unique Properties
Angewandte Chemie, EarlyView.Poly‐L‐proline is a unique polypeptide distinguished by its well‐defined secondary structures and unusual thermal behavior. This mini review highlights recent advances in the synthesis of poly‐L‐proline by N‐carboxyanhydride (NCA) polymerization, the formation of structurally defined architectures and nanostructures, and emerging strategies that ...
Rosanna Le Scouarnec +3 more
wiley +2 more sources