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Purification of glial fibrillary acidic protein (GFAP) from normal bovine brain

Journal of Neuroscience Methods, 1991
Glial fibrillary acidic protein (GFAP) was purified from normal bovine brain by a modification of the procedure used to isolate vimentin in order to avoid contamination by other cytoskeletal components: vimentin, neurofilament triplet proteins, tubulin and actin.
R, Fukuyama, S, Fushiki, S, Fujita
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Immunohistochemical demonstration of glial fibrillary acidic protein (GFAP) in nasal gliomas

Acta Histochemica, 1987
Using the Sternberger method (Immunoluk Histoset KIT) GFAP (glial fibrillary acidic protein) was demonstrated immunohistochemically in 4 nasal gliomas. In these histologically complex tumour-like lesions mesenchymal, epithelial, and neuroglial tissues as well as small groups of scattered glial elements could be differentiated specifically by the highly
B, Bozoky, D, Stiller, J, Ormos
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Measurement of glial fibrillary acidic protein (GFAP) and anti-GFAP antibodies by solid-phase radioimmunoassays

Acta Neurologica Scandinavica, 2009
A solid-phase radioimmunoassay was developed for the detection of glial fibrillary acidic protein (GFAP) and GFAP-specific immunoglobulin G (IgG) antibodies. In antibody assays purified GFAP was absorbed onto polystyrene beads, followed by incubation in dilutions of serum.
J, Ruutiainen   +4 more
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Glial Fibrillary Acidic Protein (GFAP) in Human Cerebral Tumors. An Immunohistochemical Study

Tumori Journal, 1983
Glial fibrillary acidic protein (GFAP) was studied in 160 cerebral tumors, mostly of neuro-epithelial nature. It was positive in astroglial tumors with an intensity proportional to the degree of cell differentiation. It was sometimes positive also in non-astroglial tumors, such as oligodendrogliomas and ependymomas, and this finding is discussed in ...
SCHIFFER, Davide   +3 more
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Anti-goldfish glial fibrillary acidic protein (GFAP) recognises astrocytes from rat CNS

Brain Research, 1989
A polyclonal antibody to goldfish GFAP recognises, immunohistochemically, astrocyte populations in rat brain, spinal cord and optic nerve. The pattern of staining compares favourably with that obtained using a polyclonal anti-human GFAP or a monoclonal anti-porcine GFAP.
S S, Shehab   +3 more
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Glial fibrillary acidic protein (GFAP) immunoreactivity in rabbit retina: Effect of fixation

Experimental Eye Research, 1990
The binding of monoclonal and polyclonal antibodies to glial fibrillary acidic protein (GFAP) antigenic sites in the rabbit retina was shown to be sensitive to aldehyde fixation. In chemically unfixed retina, the polyclonal anti-GFAP labeled Müller cells, astrocytes, and unidentified profiles in the outer plexiform layer; the monoclonal anti-GFAP ...
D K, Vaughan, P A, Erickson, S K, Fisher
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High ammonia diet: Its effect on the glial fibrillary acidic protein (GFAP)

Neurochemical Research, 1993
The effect of a recent hyperammonemic model, consisting of a high ammonia diet for 3, 7, 15, 45, and 90 days, on glial fibrillary acidic protein (GFAP) in the rat spinal cord and on blood ammonia levels has been studied. The high ammonia diet was prepared by mixing a standard diet with ammonium acetate (20% wt/wt); in addition, 5 mM of ammonium acetate
G, Bodega   +7 more
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A study of glial fibrillary acidic protein (GFAP) in childhood brain tumours

International Journal of Cancer, 1983
AbstractA retrospective study of 366 primary brain tumours in childhood has been carried out. GFAP was detected in juvenile astrocytoma (76/121), adult astrocytoma (34/78), malignant glioma (0/8), other astrocytic tumours (2/4) and medulloblastoma (0/101).
H B, Marsden   +3 more
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Glial fibrillary acidic protein (GFAP) from goldfish: Its localisation in visual pathway

Glia, 1989
AbstractAn intermediate filament fraction, isolated from goldfish brain, contains a prominent protein having a molecular weight of 51 kDa. In normal goldfish visual pathway, this protein is present in tectum and tract, but not in optic nerve. A polyclonal antibody raised to this protein clearly labels ependymal glial profiles in tectum and parallel ...
S N, Nona   +3 more
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Identification of spinal cord polypeptides related to glial fibrillary acidic protein (GFAP)

NeuroReport, 1992
Spinal cord cytoskeletal preparations from three different mammalian species contain several polypeptides in the SDS-PAGE molecular weight range 100-220 kDa which are recognized by all members of a panel of antibodies to glial fibrillary acidic protein (GFAP) and by anti-IFA, a panspecific intermediate filament protein antibody.
G, Shaw, J, Hawkins
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