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Immobilization of Recombinant Glucose Isomerase for Efficient Production of High Fructose Corn Syrup

Applied Biochemistry and Biotechnology, 2017
Zhi-Qiang Liu   +2 more
exaly   +2 more sources

PbrbZIP15 promotes sugar accumulation in pear via activating the transcription of the glucose isomerase gene PbrXylA1.

The Plant Journal, 2023
The composition and abundance of soluble sugars in mature pear (Pyrus) fruit are important for its acceptance by consumers. However, our understanding of the genes responsible for soluble sugar accumulation remains limited.
Luting Jia   +9 more
semanticscholar   +1 more source

Crystal structure of the metal-free state of glucose isomerase reveals its minimal open configuration for metal binding.

Biochemical and Biophysical Research Communications - BBRC, 2021
Glucose/xylose isomerase catalyzes the reversible isomerization of d-glucose and d-xylose to d-fructose and d-xylulose, respectively. This enzyme is not only involved in sugar metabolism but also has industrial applications, such as in the production of ...
K. Nam
semanticscholar   +1 more source

Enhanced catalytic efficiency and thermostability of glucose isomerase from Thermoanaerobacter ethanolicus via site-directed mutagenesis.

Enzyme and Microbial Technology, 2021
Glucose isomerase (GI) is a key enzyme in the preparation of high fructose corn syrup (HFCS). In this study, a mutant TEGI-M-L38 M/V137 L (TEGI-M2) of glucose isomerase (TEGI-M) originated from Thermoanaerobacter ethanalicus CCSD1 was obtained by site ...
Li-Qun Jin   +4 more
semanticscholar   +1 more source

Optimization of simultaneous saccharification and isomerization of dextrin to high fructose syrup using a mixture of immobilized amyloglucosidase and glucose isomerase

, 2021
High fructose syrup is a sweetener widely used as a substitute for sucrose in the food and beverage industry with its current industrial production being carried out in three sequential processes using three different enzymes that operate under different
Murilo Amaral-Fonseca   +3 more
semanticscholar   +1 more source

Permeabilized TreS-expressing Bacillus subtilis cells decorated with glucose isomerase and a shell of ZIF-8 as a reusable biocatalyst for the co-production of trehalose and fructose.

Journal of Agricultural and Food Chemistry, 2020
Metal-organic frameworks (MOFs) are a class of porous materials with versatile properties. In this study, ZIF-8 was employed to establish a two-enzyme system by encapsulating permeabilized Bacillus subtilis cells coated with glucose isomerase.
Liying Zhu   +3 more
semanticscholar   +1 more source

Thermoinactivation mechanism of glucose isomerase

Applied Biochemistry and Biotechnology, 2007
In this article, the mechanisms of thermoinactivation of glucose isomerase (GI) from Streptomyces rubiginosus (in soluble and immobilized forms) were investigated, particularly the contributions of thiol oxidation of the enzyme's cysteine residue and a "Maillard-like" reaction between the enzyme and sugars in high fructose corn syrup (HFCS). Soluble GI
Leng Hong, Lim, Bradley A, Saville
openaire   +2 more sources

Glucose - 6 - phosphate isomerase

Philosophical Transactions of the Royal Society of London. B, Biological Sciences, 1981
Abstract Glucose-6-phosphate isomerase (EC 5 . 3 .1 .9) is a dimeric enzyme of molecular mass 132000 which catalyses the interconversion of D-glucose-6-phosphate and D-fructose- 6-phosphate. The crystal structure of the enzyme from pig muscle has been determined at a nominal resolution of 2.6 A. The structure is of the a/B type.
A, Achari   +4 more
openaire   +2 more sources

Optimization of various encapsulation systems for efficient immobilization of actinobacterial glucose isomerase

, 2020
Glucose isomerase (GI) converts glucose into fructose by a reversible reaction. This reaction is industrially valuable for the production of high fructose corn syrup (HFCS) which is used as a sweetener by food and beverage industries.
T. Singh, A. Jajoo, S. Bhasin
semanticscholar   +1 more source

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