Results 61 to 70 of about 144,829 (355)

The 2.8 Å Electron Microscopy Structure of Adeno-Associated Virus-DJ Bound by a Heparinoid Pentasaccharide

Molecular Therapy: Methods & Clinical Development, 2017
Atomic structures of adeno-associated virus (AAV)-DJ, alone and in complex with fondaparinux, have been determined by cryoelectron microscopy at 3 Å resolution. The gene therapy vector, AAV-DJ, is a hybrid of natural serotypes that was previously derived
Qing Xie, John M. Spear, Alex J. Noble, Duncan R. Sousa, Nancy L. Meyer, Omar Davulcu, Fuming Zhang, Robert J. Linhardt, Scott M. Stagg, Michael S. Chapman   +9 more
doaj   +1 more source

Decoding the Human Immunoglobulin G-Glycan Repertoire Reveals a Spectrum of Fc-Receptor- and Complement-Mediated-Effector Activities

Frontiers in Immunology, 2017
Glycosylation of the immunoglobulin G (IgG)-Fc tail is required for binding to Fc-gamma receptors (FcγRs) and complement-component C1q. A variety of IgG1-glycoforms is detected in human sera.
G. Dekkers, Louise W Treffers, R. Plomp, A. Bentlage, M. de Boer, C. Koeleman, Suzanne N. Lissenberg-Thunnissen, R. Visser, M. Brouwer, J. Y. Mok, H. Matlung, T. K. van den Berg, Wim J E van Esch, T. Kuijpers, D. Wouters, T. Rispens, M. Wuhrer, G. Vidarsson   +17 more
semanticscholar   +1 more source

Expression and in vitro glycosylation of recombinant edible bird nest (EBN) mucin

Food Materials Research
Edible bird's nest (EBN) is considered a delicacy in Chinese cuisine. The nests are produced by male swiftlets (Aerodramus sp.). Due to their high price, the nutritional benefit from EBN can currently only be afforded by affluent consumers.
Ge Cheng, Yunsheng Lyu, Ran Ran, Li Liu, Josef Voglmeir   +4 more
doaj   +1 more source

Networks of HIV-1 Envelope Glycans Maintain Antibody Epitopes in the Face of Glycan Additions and Deletions

bioRxiv, 2020
Numerous broadly neutralizing antibodies (bnAbs) have been identified that target the glycans of the HIV-1 envelope spike. Neutralization breadth is notable given that glycan processing can be substantially influenced by the presence or absence of ...
Gemma E. Seabright, C. Cottrell, M. V. van Gils, Alessio D’addabbo, D. Harvey, Anna-Janina Behrens, Joel D. Allen, Yasunori Watanabe, A. Maker, Snežana Vasiljevic, Natalia de Val, R. Sanders, A. Ward, M. Crispin   +13 more
semanticscholar   +1 more source

Peptide‐based ligand antagonists block a Vibrio cholerae adhesin

FEBS Letters, EarlyView.
The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang, Grace Du, Charity Yongo‐Luwawa, Angelina Lu, Brett Kinrade, Kim Munro, Karl E. Klose, William D. Lubell, Peter Davies, Shuaiqi Guo   +9 more
wiley   +1 more source

Characterization of Cell Surface Glycan Profiles in Human and Mouse Corneas Using Lectin Microarrays

Cells, 2023
The advent of high-throughput sequencing technologies has facilitated the profiling of glycosylation genes at a single-cell level in complex biological systems, but the significance of these gene signatures to the composition of the glycocalyx remains ...
Rafael Martínez-Carrasco, Pablo Argüeso   +1 more
doaj   +1 more source

Glycan shield and epitope masking of a coronavirus spike protein observed by cryo-electron microscopy

Nature Structural &Molecular Biology, 2016
The threat of a major coronavirus pandemic urges the development of strategies to combat these pathogens. Human coronavirus NL63 (HCoV-NL63) is an α-coronavirus that can cause severe lower-respiratory-tract infections requiring hospitalization. We report
A. Walls, M. Tortorici, Brandon Frenz, J. Snijder, Wentao Li, Félix A Rey, F. DiMaio, B. Bosch, D. Veesler   +8 more
semanticscholar   +1 more source

Glycan and lectin biosensors [PDF]

Essays in Biochemistry, 2016
A short description about the importance of glycan biorecognition in physiological (blood cell type) and pathological processes (infections by human and avian influenza viruses) is provided in this review. Glycans are described as much better information storage media, compared to proteins or DNA, due to the extensive variability of glycan structures ...
 tefan Belicky, J. Katrlik, J. Tka 
openaire   +2 more sources

Glycosylated LGALS3BP is highly secreted by bladder cancer cells and represents a novel urinary disease biomarker

Molecular Oncology, EarlyView.
Urinary LGALS3BP is elevated in bladder cancer patients compared to healthy controls as detected by the 1959 antibody–based ELISA. The antibody shows enhanced reactivity to the high‐mannose glycosylated variant secreted by cancer cells treated with kifunensine (KIF).
Asia Pece, Giulio Lovato, Ilaria Cela, Arianna Mercatelli, Benedetta Ferro, Jussi Nikkola, Sara Pagotto, Tommaso Grottola, Vincenzo De Laurenzi, Rossella Cicchetti, Antonio Marchetti, Luigi Schips, Rossano Lattanzio, Stefano Iacobelli, Emily Capone, Peter Black, Mads Daugaard, Michele Marchioni, Gianluca Sala   +18 more
wiley   +1 more source

Novel PNGase H+ from Amycolatopsis mediterranei: biochemical properties and food analysis potential

Food Materials Research
Peptide-N4-(N-acetyl-β-D-glucosaminyl)asparagine amidases (PNGases) are essential biocatalysts for the deglycosylation of complex carbohydrates from glycoproteins, protein analysis and food quality assessment.
Bingqian Wei, Li Liu, Josef Voglmeir
doaj   +1 more source