Results 161 to 170 of about 88,542 (309)

IgG Glycosylation‐Dependent CLEC7A Signaling Drives Podocyte Dysfunction in Lupus Nephritis

Arthritis &Rheumatology, EarlyView.
Objective Lupus nephritis (LN) is a severe complication of systemic lupus erythematosus (SLE) that can lead to end‐stage kidney disease and increased mortality. IgG from patients with LN displays abnormal glycosylation, contributing to podocyte injury.
Rohit Upadhyay, Alexia Orellana, George C. Tsokos, Rhea Bhargava   +3 more
wiley   +1 more source

Ferritin as a Nanoparticle Scaffold for Plant‐Produced Next‐Generation Subunit Vaccines

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Protein nanoparticles offer an innovative approach to next‐generation subunit vaccine development by displaying antigenic sequences on the nanoparticle surface. Compared to traditional subunit vaccines, protein nanoparticle vaccines often show improved interaction with the immune system due to their particulate size and repetitive epitope ...
Jordan T. VanderBurgt, Richard Strasser, Christopher P. Garnham, Rima Menassa   +3 more
wiley   +1 more source

Biological recognition of mirror-image glycans

Recent synthesis of essential enzymes, such as DNA and RNA polymerases with opposite chirality, has boosted the feasibility of creating mirror-image life.
Christoph Rademacher, Landon J. Edgar, Lori J. West, Claire Yi-ling Yang, Jonathan Lefebre, Ching-Ching Yu, Chuanhao Peng, Lara K. Mahal, Michael Evjen, Vanessa Affe, Matthew S. Macauley, Eric J. Carpenter, Ratmir Derda, Carolina Ortiz-Cordero, Mei-Ting Lin, Laura L. Kiessling, Sheng-Kai Wang, Simatsidk Haregu, Hani Choksi, Ayodeji Kulepa, Szu Yun Wang, Sunhee Bae, Shreyas Gupta, Nicholas Twells, Todd L. Lowary, Alexxandra Sosa-Guir   +25 more
core   +1 more source

Characterization of Immune Responses to RBD‐SD1 Nanoparticle Vaccines Derived From ACE2‐Capable HKU5‐CoV‐2, NeoCoV, and HKU5 Merbecoviruses

Biotechnology and Bioengineering, EarlyView.
SpyCatcher‐mi3 nanoparticles presenting HKU5, NeoCoV, and HKU5‐CoV‐2 RBD‐SD1 induced strong, cross‐reactive IgG responses in immunized mice. The surrogate virus neutralization test demonstrated strong activity in sera from homologous vaccination. ABSTRACT Recent discoveries of merbecoviruses such as HKU5, NeoCoV, and HKU5‐CoV‐2 that use angiotensin ...
Peter J. Halfmann, Jeong Soo Lee, Augustine Duffy, Tong Wang, Bingcheng Huang, James Z. Kurdi, Ajay Inampudi, Natasha Chen, Elizabeth Frimpong, Carmen Rai, Yoshihiro Kawaoka, Ravi S. Kane   +11 more
wiley   +1 more source

Editorial: The role of glycans in infectious disease, Volume II

Frontiers in Microbiology, 2022
Iván Martínez-Duncker, Héctor M. Mora-Montes, Gerardo R. Vasta, Fabrizio Chiodo, Fabrizio Chiodo   +4 more
doaj   +1 more source

Unmasking Glycoforms: Lectin‐Based Profiling and Functional Implications of Targeted Glycosylation Knockouts in Chinese Hamster Ovary Cells

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Monoclonal antibody (mAb) glycosylation is a critical quality attribute that is difficult to rationally engineer and rapidly assess during cell line development. Here, we investigate whether cell‐surface glycosylation can serve as a surrogate readout of mAb product glycosylation following targeted glycogene engineering in CHO cells.
Cristina Abascal Ruiz, Sheryl Li Yan Lim, Jacobus Brink, Sara Carillo, Eoin Casey, Jonathan Bones, Ioscani Jiménez del Val   +6 more
wiley   +1 more source

IgG N-glycosylation in autoimmune diseases. [PDF]

Front Immunol
Pumpe C, Vermeren S.
europepmc   +1 more source

GlycoMAC: A Multiscale Metabolic‐Glycosylation Framework for Predicting Glycosylation Across Conditions in Mammalian Cell Cultures

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Antibody productivity and glycosylation quality in CHO cell cultures emerge from a dynamically evolving metabolic environment, yet existing models often work in isolation or at a single scale. Here, we present a multiscale mechanistic framework linking molecular, cellular, and macroscopic scales to predict how inputs shape bioprocess ...
Yuming Zeng, Sarah W. Harcum, Jinxiang Pei, Wei Xie   +3 more
wiley   +1 more source

HPLC separation of N-glycans

HPLC separation of N-glycans (fucosylated and non-fucosylated trimannosyl core of N-glycans) extracted from HEK293T cells wild-type (WT); deficient in TSTA3 protein (TSTA3KO); deficient in GMDS (GMDSKO) protein; deficient in FCSK (FCSKKO) protein treated
Skurska, Edyta
core   +1 more source

Batch‐to‐Batch Optimization of pH Shifts in CHO Bioreactor Operations

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Culture pH is an important operating parameter in CHO cell cultures producing mAb since it directly affects growth and specific productivity. Mathematical models can be used to optimize the magnitude and timing of pH shifts provided that they are robust to model errors.
Ali Ghodba, Zahra Negahban, Anne Richelle, Chris McCready, Valerie Ward, Luis Ricardez‐Sandoval, Hector Budman   +6 more
wiley   +1 more source