Influence of in situ progressive N-terminal is still controversial truncation of glycogen branching enzyme in Escherichia coli DH5α on glycogen structure, accumulation, and bacterial viability. [PDF]
BMC Microbiol, 2015 BackgroundGlycogen average chain length (ACL) has been linked with bacterial durability, but this was on the basis of observations across different species. We therefore wished to investigate the relationship between bacterial durability and glycogen ACL
Wang L +6 more
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Molecules, 2023 Glycogen is the primary storage polysaccharide in bacteria and animals. It is a glucose polymer linked by α-1,4 glucose linkages and branched via α-1,6-linkages, with the latter reaction catalyzed by branching enzymes. Both the length and dispensation of
Remie Fawaz +6 more
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The versatile activity of glycogen branching enzymes [PDF]
, 2020 Glycogen branching enzymes (GBEs) are active in the biosynthesis of glycogen and can be applied to modify starch. These enzymes have been described to introduce new branch points but their exact mechanism is still little understood due to the size and complexity of both their substrate and product.
Lucie Gänssle
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PLoS Computational Biology, 2023 In humans, glycogen storage diseases result from metabolic inborn errors, and can lead to severe phenotypes and lethal conditions. Besides these rare diseases, glycogen is also associated to widely spread societal burdens such as diabetes.
Yvan Rousset +2 more
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Stochastic modelling of a three-dimensional glycogen granule synthesis and impact of the branching enzyme [PDF]
bioRxiv, 2022 In humans, glycogen storage diseases result from metabolic inborn errors, and can lead to severe phenotypes and lethal conditions. Besides these rare diseases, glycogen is also associated to widely spread societal burdens such as diabetes.
Yvan Rousset +2 more
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Expression of
Plant, Cell and Environment, 2015 Starch synthesis requires several enzymatic activities including branching enzymes (BEs) responsible for the formation of α(1 → 6) linkages. Distribution and number of these linkages are further controlled by debranching enzymes that cleave some of them,
Laura Boyer +10 more
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The Unique Branching Patterns of Deinococcus Glycogen Branching Enzymes Are Determined by Their N-Terminal Domains [PDF]
Applied and Environmental Microbiology, 2009 ABSTRACT Glycogen branching enzymes (GBE) or 1,4-α-glucan branching enzymes (EC 2.4.1.18) introduce α-1,6 branching points in α-glucans, e.g., glycogen. To identify structural features in GBEs that determine their branching pattern specificity, the Deinococcus geothermalis and Deinococcus ...
Marta Palomo +3 more
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PLoS ONE, 2019 Glycogen is a highly branched α-glucan polymer widely used as energy and carbon reserve by many microorganisms. The branches are introduced by glycogen branching enzymes (EC 2.4.1.18), that are classified into glycoside hydrolase families 13 (GH13) and ...
Xuewen Zhang +2 more
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Purification and Subunit Structure of Glycogen-Branching Enzyme from Rabbit Skeletal Muscle [PDF]
European Journal of Biochemistry, 1980 1,4-alpha-glucan:1,4-alpha-glucan 6-alpha-D-(1,4-alpha-D-glucano) transferase (branching enzyme) was purified by ammonium sulphate precipitation, chromatography on DEAE-cellulose, fractionation with poly(ethyleneglycol) 6000, chromatography on DEAE-Sepharose and gel filtration on Sephadex G150. The final specific activity was 3000 U/mg corresponding to
F. Barry Caudwell, Philip Cohen
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Synthesis of highly branched α-glucans with different structures using GH13 and GH57 glycogen branching enzymes [PDF]
Carbohydrate Polymers, 2019 Glycogen branching enzymes (GBEs) convert starch into branched α-glucan polymers. To explore if the amylose content of substrates effects the structure of the branched α-glucans, mixtures of amylose and amylopectin were converted by four thermophilic GBEs.
Xuewen Zhang +2 more
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