Progress in the Detection of Erythropoietin in Blood, Urine, and Tissue
Detection of erythropoietin (Epo) was difficult until a method was developed by the World Anti-Doping Agency (WADA). WADA recommended the Western blot technique using isoelectric focusing (IEF)-PAGE to show that natural Epo and injected erythropoiesis ...
Yukiko Yasuoka +4 more
doaj +1 more source
Rabies virus glycoprotein produced in Nicotiana benthamiana is an immunogenic antigen in mice
Rabies remains an infectious disease among humans and animals, and requires the development of an effective vaccine essential to prevent rabies. Advances in molecular biology and biotechnology have led to the development and improvement of many rabies ...
Youngmin Park +10 more
doaj +1 more source
The surface glycoproteins of hantaviruses
Hantaviruses are rodent-borne viruses distributed worldwide, transmitted through the air and with the ability to spread from person to person. They maintain a non-symptomatic persistent infection in their rodent hosts, but their spillover to humans produces a renal or pulmonary syndrome associated with high fatality rates.
Guardado-Calvo, Pablo, Rey, Félix
openaire +3 more sources
Analysis of Determinants in Filovirus Glycoproteins Required for Tetherin Antagonism
The host cell protein tetherin can restrict the release of enveloped viruses from infected cells. The HIV-1 protein Vpu counteracts tetherin by removing it from the site of viral budding, the plasma membrane, and this process depends on specific ...
Kerstin Gnirß +7 more
doaj +1 more source
Vesicular stomatitis virus glycoprotein is necessary for H-2-restricted lysis of infected cells by cytotoxic T lymphocytes [PDF]
Vesicular stomatitis virus (VSV) elicited cytotoxic thymus-derived lymphocytes (CTLs) in mice of the BALB/c and three congenic strains (BALB.b, BALB.k, BALB.HTG).
Baltimore, David +3 more
core +2 more sources
Filovirus serological diagnosis and epidemiological investigations are hampered due to the unavailability of validated immunoassays. Diagnostic performance of three indirect enzyme-linked immunosorbent assays (I-ELISA) was evaluated for the detection of ...
Janusz T. Paweska +6 more
doaj +1 more source
Mutations in the Schmallenberg virus Gc glycoprotein facilitate cellular protein synthesis shutoff and restore pathogenicity of NSs deletion mutants in mice [PDF]
Serial passage of viruses in cell culture has been traditionally used to attenuate virulence and identify determinants of viral pathogenesis. In a previous study, we found that a strain of Schmallenberg virus (SBV) serially passaged in tissue culture ...
Aislynn Taggart +13 more
core +1 more source
Proteins following the secretory pathway acquire their proper tertiary and in certain cases also quaternary structures in the endoplasmic reticulum (ER). Incompletely folded species are retained in the ER and eventually degraded. One of the molecular mechanisms by which cells achieve this conformational sorting is based on monoglucosylated N-glycans ...
Trombetta, E. Sergio +1 more
openaire +3 more sources
The S-layer protein of the anammox bacterium Kuenenia stuttgartiensis is heavily O-glycosylated
Anammox bacteria are a distinct group of Planctomycetes that are characterized by their unique ability to perform anaerobic ammonium oxidation with nitrite to dinitrogen gas in a specialized organelle.
Muriel C.F. van Teeseling +8 more
doaj +1 more source
Glycoprotein In Vitro N-Glycan Processing Using Enzymes Expressed in E. coli
Protein N-glycosylation is a common post-translational modification that plays significant roles on the structure, property, and function of glycoproteins.
Libo Zhang +7 more
doaj +1 more source

