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Glycoside hydrolases: Catalytic base/nucleophile diversity

Biotechnology and Bioengineering, 2010
AbstractRecent studies have shown that a number of glycoside hydrolase families do not follow the classical catalytic mechanisms, as they lack a typical catalytic base/nucleophile. A variety of mechanisms are used to replace this function, including substrate‐assisted catalysis, a network of several residues, and the use of non‐carboxylate residues or ...
Thu V, Vuong, David B, Wilson
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Twisting of glycosidic bonds by hydrolases

Carbohydrate Research, 2009
Patterns of scissile bond twisting have been found in crystal structures of glycoside hydrolases (GHs) that are complexed with substrates and inhibitors. To estimate the increased potential energy in the substrates that results from this twisting, we have plotted torsion angles for the scissile bonds on hybrid Quantum Mechanics::Molecular Mechanics ...
Johnson, Glenn   +3 more
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Promoting and Impeding Effects of Lytic Polysaccharide Monooxygenases on Glycoside Hydrolase Activity

, 2020
Lytic polysaccharide monooxygenases (LPMOs) have attracted attention due to their ability to boost cellulolytic enzyme cocktails for application in biorefineries.
M. B. Keller   +5 more
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Glycosyltransferases, glycoside hydrolases: surprise, surprise!

Current Opinion in Structural Biology, 2008
Several in the field-and many outside-consider that solving the three-dimensional structures of more glycoside hydrolases (GHs) and glycosyltransferases (GTs) confines to stamp collection and some even think that there is no main revelation to expect in this area. It is wrong! The past year has come as a refreshing wake-up call with major surprises for
Henrissat, Bernard   +2 more
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Hierarchical classification of glycoside hydrolases

Biochemistry (Moscow), 2011
This review deals with structural and functional features of glycoside hydrolases, a widespread group of enzymes present in almost all living organisms. Their catalytic domains are grouped into 120 amino acid sequence-based families in the international classification of the carbohydrate-active enzymes (CAZy database).
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