Results 211 to 220 of about 232,627 (303)

Advanced glucose control strategies leveraging Raman spectroscopy for optimized mammalian cell culture manufacturing

Biotechnology Progress, EarlyView.
Abstract Maintaining consistent quality in the manufacturing of biotherapeutic proteins in mammalian cell culture is challenging, with unplanned deviations causing inconsistencies and potential batch failure. Current methods for monitoring and controlling critical process parameters (CPPs) rely on slow, labor‐intensive offline analyses.
Matthew Banner, Daniel Ray, Irfan Ahanger, Fabio Zurlo, Noah Hitchcock, Rajesh Mistry, Kasia Kozakowska‐McDonnell, Matthew Cheeks, Richard Turner, Jonathan Welsh, Suzanne S. Farid, Michael Thomas, Stephen Goldrick   +12 more
wiley   +1 more source

Post-translational modifications in CD8⁺ T cell exhaustion: mechanisms, networks, and epigenetic locking. [PDF]

Front Immunol
Yu T, Xia M, Wu Y, Wu Q, Xie J, Gao H, Hu S.   +6 more
europepmc   +1 more source

Screening of bispecific antibodies during single cell cloning of recombinant Chinese hamster ovary cell lines to improve titer and product quality

Biotechnology Progress, EarlyView.
Abstract Development of therapeutic bispecific antibodies (BsAbs) poses significant manufacturability challenges associated with correct chain pairing and an increased diversity of impurities. Here we address this with a product quality assessment during cell line development (CLD), forgoing the need for genetic characterization during cell line ...
Molly Robinson, Jessica Willmott, Holly Corrigall, Daniela Lega, Joseph Stirling, Vithiya Vimalraj, Mitul Patel, Robyn Emmins   +7 more
wiley   +1 more source

Human Antibody Isotypes, Subclasses, Allotypes and Fc Biology: Clinical Implications in Infectious Diseases, Autoimmunity, and Cancer. [PDF]

Pharmaceuticals (Basel)
Gautam S, Singh S, Thakkar V, Joshi D, Kumar S.   +4 more
europepmc   +1 more source

Protein–Anthocyanin Interactions in Food Systems: A Reaction‐Network Perspective on Equilibria, Kinetics, and Degradation Pathways

ChemFoodChem, EarlyView.
Protein–anthocyanin interactions are reinterpreted beyond binding affinity through a reaction‐network perspective. Distinct protein classes modulate anthocyanin behavior through equilibrium shifts, matrix‐driven protection, self‐assembly, or covalent conjugation.
E. Mariana Martins, Victor de Freitas, Susana Soares, Joana Oliveira   +3 more
wiley   +1 more source

Transcriptome, glycome, and mucinome analysis reveal zinc is essential for the composition of mucus in the human goblet cell model HT-29-MTX. [PDF]

Sci Rep
Schüßler C, Chung N, Léonard R, Sprenger H, Rödel T, Mahoney KE, Thomsen S, Ocket E, Matthaeus C, Denis J, Ebert F, Wolf M, Morelle W, Foulquier F, Braeuning A, Masselot CR, Malaker SA, Maares M.   +17 more
europepmc   +1 more source

Metal‐Ion Regulated Light‐Free Z→E Isomerization of Azobenzene Glycomacrocycle

Chemistry – A European Journal, EarlyView.
Metal ion‐catalyzed Z→E isomerization of photoswitchable azobenzene glycomacrocycle: A new azobenzene‐based glycomacrocycle is capable of forming a 1:1 complex with divalent metal ions in both E‐ and Z‐isomers. The complexes undergo light‐free Z→E isomerization in a timescale spanning from seconds to weeks.
Yang Zhou, Jinbiao Jiao, Mathieu Lordez, Jean‐Yves Salpin, Juan Xie   +4 more
wiley   +1 more source

UGT707B2 From Iris tectorum Maxim.: A Promiscuous Glycosyltransferase for the Biosynthesis of Structurally Diverse Flavonoid Glycosides. [PDF]

Chembiochem
Zhang L, Li J, Zhang Q, Zhang L, Wang Y, Ji A, Huang J.   +6 more
europepmc   +1 more source

GH172 Difructose Dianhydride I Synthase: A Two‐Stepped Journey via Glycosylation and Cyclization Using QM/MM Metadynamics

Chemistry – A European Journal, EarlyView.
Unlocking the chemistry of caramel by simulations and experimental validation. αFFase1${\alpha }\rm{FFase1}$ converts inulobiose into difructose dianhydride I, one component of caramel, through two steps: a rate‐limiting glycosylation (with a rare sugar ring flip) followed by a cyclization step, guided by residues E85, K147, and N226.
Santiago Alonso‐Gil, Bruno Di Geronimo, Toma Kashima, Akihiro Ishiwata, Katsunori Tanaka, Kiyotaka Fujita, Shinya Fushinobu, Pedro A. Sánchez‐Murcia   +7 more
wiley   +1 more source

Shared glycosylation-related molecular mechanism and immune infiltration patterns of fetal growth restriction and preeclampsia. [PDF]

Front Immunol
Yin T, Hu X, Yu H, Zhang T, Yu Q, Huang X, Xu C, Tang C, Liu L, Du M.   +9 more
europepmc   +1 more source