Results 111 to 120 of about 602 (161)
A Glycosynthase Catalyst for the Synthesis of Flavonoid Glycosides
Angewandte Chemie - International Edition, 2007 Glycosylation of lipophilic small molecules is one of the predominant strategies by which the bioactivity of these compounds is modulated in living organisms. Indeed, even simple plants, such as Arabidopsis thaliana, dedicate over 120 open reading frames encoding many activated sugar-dependent glycosyltransferases to this task alone.
Ben G. Davis, Min Yang, Gideon Davies
exaly +4 more sources
Some of the next articles are maybe not open access.
Related searches:
Related searches:
Glycosynthase Mediated Synthesis of Psychosine
Carbohydrate Research, 2016Globoid cell leukodystrophy (GCL), or Krabbe disease, is a lysosomal storage disorder characterized by a deficiency in galactosylceramidase (GALC), which hydrolyses galactosylceramide and galactosylsphingosine (psychosine). Early detection of GCL in newborns is essential for timely therapeutic intervention and could be achieved by testing infant blood ...
Stephen Withers +2 more
exaly +3 more sources
Oligosaccharide synthesis by glycosynthases
Trends in Biotechnology, 2004Oligosaccharide synthesis is becoming increasingly important to the pharmaceutical industry because these biomolecules have potential application as therapeutics. Enzymatic synthesis of oligosaccharides includes both glycosyl transferases and glycosidases.
Perugino G +3 more
openaire +4 more sources
Biochemistry, 2011
Understanding how enzymes mediate catalysis is a key to their reprogramming for biotechnological applications. The family 3 retaining glycosidase postulated to be involved in erythromycin self-resistance was cloned, recombinantly expressed in Escherichia coli, purified, and characterized.
David L, Jakeman, Ali, Sadeghi-Khomami
openaire +2 more sources
Understanding how enzymes mediate catalysis is a key to their reprogramming for biotechnological applications. The family 3 retaining glycosidase postulated to be involved in erythromycin self-resistance was cloned, recombinantly expressed in Escherichia coli, purified, and characterized.
David L, Jakeman, Ali, Sadeghi-Khomami
openaire +2 more sources
Alternative Strategy for Converting an Inverting Glycoside Hydrolase Into a Glycosynthase. [PDF]
Glycobiology, 2008 The tyrosine residue Y198 is known to support a nucleophilic water molecule with the general base residue, D263, in the reducing-end xylose-releasing exo-oligoxylanase (Rex). A mutation in the tyrosine residue changing it into phenylalanine caused a drastic decrease in the hydrolytic activity and a small increase in the F(-) releasing activity from ...
Motomitsu Kitaoka +2 more
exaly +3 more sources
Development of a colourimetric assay for glycosynthases
Journal of Biotechnology, 2017The synthesis of glycosidic structures by catalysis via glycosynthases has gained much interest due to the potential high product yields and specificity of the enzymes. Nevertheless, the characterisation and implementation of new glycosynthases is greatly hampered by the lack of high-throughput methods for reaction analysis and screening of potential ...
Marc R, Hayes +4 more
openaire +2 more sources
Glycosynthase-Mediated Synthesis of Glycosphingolipids
Journal of the American Chemical Society, 2006Glycosphingolipids play crucial roles in virtually every stage of the cell cycle, and their clinical administration has been proposed as a treatment for Alzheimer's, Parkinson's, stroke, and a range of other conditions. However, lack of supply has severely hindered testing of this potential.
Mark D, Vaughan +5 more
openaire +2 more sources
Thermophilic Glycosynthases for Oligosaccharides Synthesis
2012Glycosynthases are engineered glycoside hydrolases that in suitable reaction conditions promote the synthesis of oligosaccharides with exquisite stereoselectivity and enhanced regioselectivity, if compared to traditional chemical methods. This approach was demonstrated to be successful in a number of cases including β-glycosynthases acting at the ...
Cobucci-Ponzano, Beatrice +4 more
openaire +5 more sources
Structural explanation for the acquisition of glycosynthase activity
The Journal of Biochemistry, 2010Glycosynthases are engineered glycoside hydrolases (GHs) that catalyse the synthesis of glycoside from glycosyl-fluoride donors and suitable acceptors. We have determined five crystal structures of the glycosynthase mutants reducing-end xylose-releasing exo-oligoxylanase, an inverting GH, that exhibit various levels of glycosynthetic activities. At the
Masafumi, Hidaka +5 more
openaire +2 more sources
Enzymatic transglycosylation of xylose using a glycosynthase
Carbohydrate Research, 2005The application of the hyperactive glycosynthase derived from Agrobacterium sp. beta-glucosidase (AbgE358G-2F6) to the synthesis of xylo-oligosaccharides by using alpha-D-xylopyranosyl fluoride as donor represents the first successful use of glycosynthase technology for xylosyl transfer.
Young-Wan, Kim +2 more
openaire +2 more sources