Results 121 to 130 of about 602 (161)

Glycosynthases: Mutant Glycosidases for Glycoside Synthesis

Australian Journal of Chemistry, 2002
Glycosynthases are engineered mutant glycosidases that catalyse the formation of a glycosidic bond from a glycosyl donor and an acceptor alcohol. They are constructed by mutation of the enzymic nucleophile of a retaining glycosidase to a small non-nucleophilic residue. To date, five glycosynthases have been reported capable of synthesizing a range of ß-
Spencer J. Williams, Stephen G. Withers
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ChemInform Abstract: Glycosynthases in Biocatalysis.

ChemInform, 2011
AbstractReview: ca. 115 refs.
Beatrice Cobucci‐Ponzano, Andrea Strazzulli, Mose Rossi, Marco Moracci   +3 more
openaire   +1 more source

1.6.1 Glycosidases and Glycosynthases

2015
AbstractEnzymatic synthesis of glycans, as an alternative to classical chemical synthesis, is of great interest due to the exquisite stereospecificity and improved processivity and regioselectivity of the biological catalysts, and for the possibility of using reagents less toxic to the environment.
B. Cobucci Ponzano, M. Moracci
openaire   +1 more source

In vitro synthesis of artificial polysaccharides by glycosidases and glycosynthases

Carbohydrate Research, 2007
Artificial polysaccharides produced by in vitro enzymatic synthesis are new biomaterials with defined structures that either mimic natural polysaccharides or have unnatural structures and functionalities. This review summarizes recent developments in the in vitro polysaccharide synthesis by endo-glycosidases, grouped in two major strategies: (a) native
Magda, Faijes, Antoni, Planas
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Directed Evolution of a Glycosynthase via Chemical Complementation

Journal of the American Chemical Society, 2004
Recently, we reported a general assay for enzyme catalysis based on the yeast three-hybrid assay, Chemical Complementation, which is intended to expand the range of chemical reactions to which directed evolution can be applied. Here, Chemical Complementation was applied to a glycosynthase derived from a retaining glycosidase, an important class of ...
Hening, Lin, Haiyan, Tao, Virginia W, Cornish   +2 more
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Glycosynthase‐Mediated Assembly of Xylanase Substrates and Inhibitors

ChemBioChem, 2011
AbstractAn exo‐β‐xylosidase mutant with glycosynthase activity was created to aid in the synthesis of xylanase substrates and inhibitors. Simple monosaccharides were easily elaborated into di‐, tri‐ and tetrasaccharides by using this enzyme. Some products proved to be surprisingly potent inhibitors of xylanases from glycoside hydrolase families 10 and ...
Ethan D, Goddard-Borger, Brigitte, Fiege, Emily M, Kwan, Stephen G, Withers   +3 more
openaire   +2 more sources

Activity of Hyperthermophilic Glycosynthases Is Significantly Enhanced at Acidic pH

Biochemistry, 2003
We have previously shown that the hyperthermophilic glycosynthase from Sulfolobus solfataricus (Ssbeta-glyE387G) can promote the synthesis of branched oligosaccharides from activated beta-glycosides, at pH 6.5, in the presence of 2 M sodium formate as an external nucleophile.
Perugino G, Trincone A, Giordano A, van der Oost J, Kaper T, Rossi M, Moracci M   +6 more
openaire   +6 more sources

Exploitation of β-glycosyl azides for the preparation of α-glycosynthases

Biocatalysis and Biotransformation, 2012
An interesting approach for the chemo-enzymatic synthesis of carbohydrates is the use of glycosynthases, a class of mutant glycosidases derived from β-glycoside hydrolases obtained by replacement of the active site nucleophile with a non-nucleophilic residue.
Cobucci Ponzano B., Zorzetti C., STRAZZULLI, ANDREA, BEDINI, EMILIANO, CORSARO, MARIA MICHELA, Sulzenbacher G., Rossi M., MORACCI, Marco   +7 more
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Glycosynthases: new enzymes for oligosaccharide synthesis

Journal of Molecular Catalysis B: Enzymatic, 2001
The mutation of putative acidrbase and nucleophile of the active sites of retaining glycosyl hydrolases, together with kinetic analysis of the mutants, and stereochemical identification of products lead to useful information for the understanding of the reaction mechanism of these enzymes.
Moracci M, Trincone A, Rossi, M
openaire   +5 more sources

Glycosidases and Glycosynthases

2014
Chemoenzymatic synthesis of glycans as an alternative to classical chemical synthesis has great interest for the exquisite sterospecificity and improved processivity and regioselectivity of the biological catalysts and for the possibility of using reagents less toxic for the environment.
Beatrice CobucciPonzano, Marco Moracci
openaire   +3 more sources