Results 131 to 140 of about 1,170 (199)

Glycan Remodeling of Human Erythropoietin (EPO) Through Combined Mammalian Cell Engineering and Chemoenzymatic Transglycosylation. [PDF]

ACS Chem Biol, 2017
Yang Q, An Y, Zhu S, Zhang R, Loke CM, Cipollo JF, Wang LX.   +6 more
europepmc   +1 more source

Discovery of a single-subunit oligosaccharyltransferase that enables glycosylation of full-length IgG antibodies in bacteria. [PDF]

Nat Commun
Sotomayor B, Donahue TC, Mahajan SP, Taw MN, Hulbert SW, Bidstrup EJ, Owitipana DN, Pang A, Yang X, Ghosal S, Alabi CA, Azadi P, Gray JJ, Jewett MC, Wang LX, DeLisa MP.   +15 more
europepmc   +1 more source

Glycan pairing in therapeutic IgG orchestrates Fcγ receptor engagement and ADCC: an integrated structure-function approach for thorough evaluation of Fc N-glycans as critical quality attributes. [PDF]

MAbs
Meudt M, Baumeister J, Russell AC, Dzielak L, Hansen G, Mizaikoff B, Ebert S, Rosenau F, Blech M, Higel F.   +9 more
europepmc   +1 more source

Probiotic Lactobacillus acidophilus NCFM and Bifidobacterium animalis subsp lactis Bl-04 interactions with prebiotic carbohydrates using differential proteomics and protein characterization [PDF]

, 2012
Hansen, Morten Ejby
core  

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Glycosynthase mediated synthesis of psychosine.

Carbohydrate Research, 2016
Globoid cell leukodystrophy (GCL), or Krabbe disease, is a lysosomal storage disorder characterized by a deficiency in galactosylceramidase (GALC), which hydrolyses galactosylceramide and galactosylsphingosine (psychosine). Early detection of GCL in newborns is essential for timely therapeutic intervention and could be achieved by testing infant blood ...
E. Goddard-Borger, C. Tysoe, S. Withers
semanticscholar   +3 more sources

Auxiliary active site mutations enhance the glycosynthase activity of a GH18 chitinase for polymerization of chitooligosaccharides.

Carbohydrate Polymers, 2021
Depolymerization of chitin results in chitooligosaccharides (COS) that induce immunostimulatory effects and disease protective responses and have many potential applications in agriculture and medicine.
Cristina Alsina, E. Sancho-Vaello, A. Aranda-Martínez, M. Faijes, A. Planas   +4 more
semanticscholar   +1 more source

Click-Chemistry-Based Free Azide versus Azido Sugar Detection Enables Rapid In Vivo Screening of Glycosynthase Activity.

ACS Chemical Biology, 2021
Engineering of carbohydrate-active enzymes such as glycosynthases to enable chemoenzymatic synthesis of bespoke oligosaccharides has been limited by the lack of suitable ultrahigh-throughput screening methods capable of robustly detecting either starting
Ayushi Agrawal, C. K. Bandi, Tucker Burgin, Y. Woo, H. Mayes, S. Chundawat   +5 more
semanticscholar   +1 more source

Carbohydrate-binding module assisting glycosynthase-catalysed polymerizations.

Biochemical Journal, 2015
Carbohydrate-binding modules (CBMs) are found within multi-modular polysaccharide degrading enzymes [glycoside hydrolases (GHs)]. CBMs play a critical role in the recognition of plant cell-wall polysaccharides and enhance the hydrolase activity of their cognate catalytic domains by increasing enzyme substrate proximity.
V. Codera, H. Gilbert, M. Faijes, A. Planas   +3 more
semanticscholar   +4 more sources

Transglycosidase activity of glycosynthase-type mutants of a fungal GH20 β-N-acetylhexosaminidase.

International Journal of Biological Macromolecules, 2020
β-N-Acetylhexosaminidases (CAZy GH20, EC 3.2.1.52) are exo-glycosidases specific for cleaving N-acetylglucosamine and N-acetylgalactosamine moieties of various substrates. The β-N-acetylhexosaminidase from the filamentous fungus Talaromyces flavus (TfHex)
J. Kapešová, L. Petrásková, Natalia Kulik, Z. Straková, P. Bojarová, K. Markošová, M. Rebroš, V. Křen, K. Slámová   +8 more
semanticscholar   +1 more source