Results 141 to 150 of about 1,170 (199)

Oligosaccharide synthesis by glycosynthases

Trends in Biotechnology, 2004
Oligosaccharide synthesis is becoming increasingly important to the pharmaceutical industry because these biomolecules have potential application as therapeutics. Enzymatic synthesis of oligosaccharides includes both glycosyl transferases and glycosidases.
Perugino G, Trincone A, Rossi M, Moracci M   +3 more
openaire   +4 more sources

Functionalized Celluloses with Regular Substitution Pattern by Glycosynthase-Catalyzed Polymerization.

Biomacromolecules, 2016
Control of the monomer sequence in polymers is extraordinarily difficult by chemical synthesis, though Nature routinely exerts such control, including in the biosynthesis of polysaccharides. This inability has prevented us from being able to match the exquisite structure-activity control exhibited in biosynthesis of bioactive natural polysaccharides ...
V. Codera, K. Edgar, M. Faijes, A. Planas   +3 more
semanticscholar   +3 more sources

Glycosynthases in Biocatalysis

Advanced Synthesis & Catalysis, 2011
AbstractGlycosynthases, engineered glycoside hydrolases that are able to synthesize glycans in quantitative yields without hydrolyzing them, are among the most interesting tools for the chemoenzymatic synthesis of carbohydrates that have been made available so far.
Cobucci Ponzano, Beatrice, STRAZZULLI, ANDREA, ROSSI, MOSE', MORACCI, Marco   +3 more
openaire   +5 more sources

Glycosynthase-Mediated Synthesis of Glycosphingolipids

Journal of the American Chemical Society, 2006
Glycosphingolipids play crucial roles in virtually every stage of the cell cycle, and their clinical administration has been proposed as a treatment for Alzheimer's, Parkinson's, stroke, and a range of other conditions. However, lack of supply has severely hindered testing of this potential.
Mark D, Vaughan, Karl, Johnson, Shawn, DeFrees, Xiaoping, Tang, R Antony J, Warren, Stephen G, Withers   +5 more
openaire   +2 more sources

Glycosynthase Principle Transformed into Biocatalytic Process Technology: Lacto-N-triose II Production with Engineered exo-Hexosaminidase

ACS Catalysis, 2019
Glycosynthases are promising enzyme catalysts for glycoside synthesis. Derived from glycoside hydrolases by mechanistic repurposing of their active site, glycosynthases utilize suitably activated g...
Katharina Schmölzer, M. Weingarten, K. Baldenius, B. Nidetzky   +3 more
semanticscholar   +1 more source

Glycosynthase-type GH18 mutant chitinases at the assisting catalytic residue for polymerization of chitooligosaccharides.

Carbohydrate Research, 2019
Chitooligosaccharides (COS), the depolymerization products of chitin, have many potential applications in agriculture and medicine since they induce immunostimulating effects and disease protective responses.
Cristina Alsina, M. Faijes, A. Planas
semanticscholar   +1 more source

Thermophilic Glycosynthases for Oligosaccharides Synthesis

2012
Glycosynthases are engineered glycoside hydrolases that in suitable reaction conditions promote the synthesis of oligosaccharides with exquisite stereoselectivity and enhanced regioselectivity, if compared to traditional chemical methods. This approach was demonstrated to be successful in a number of cases including β-glycosynthases acting at the ...
Cobucci-Ponzano, Beatrice, Perugino, Giuseppe, Strazzulli, Andrea, Rossi, Mosè, Moracci, Marco   +4 more
openaire   +5 more sources

Glycosynthases: Mutant Glycosidases for Glycoside Synthesis

Australian Journal of Chemistry, 2002
Glycosynthases are engineered mutant glycosidases that catalyse the formation of a glycosidic bond from a glycosyl donor and an acceptor alcohol. They are constructed by mutation of the enzymic nucleophile of a retaining glycosidase to a small non-nucleophilic residue. To date, five glycosynthases have been reported capable of synthesizing a range of ß-
Spencer J. Williams, Stephen G. Withers
openaire   +1 more source

A β-(1,2)-Glycosynthase and an Attempted Selection Method for the Directed Evolution of Glycosynthases

Biochemistry, 2011
Understanding how enzymes mediate catalysis is a key to their reprogramming for biotechnological applications. The family 3 retaining glycosidase postulated to be involved in erythromycin self-resistance was cloned, recombinantly expressed in Escherichia coli, purified, and characterized.
David L, Jakeman, Ali, Sadeghi-Khomami
openaire   +2 more sources

ChemInform Abstract: Glycosynthases in Biocatalysis.

ChemInform, 2011
AbstractReview: ca. 115 refs.
Beatrice Cobucci‐Ponzano, Andrea Strazzulli, Mose Rossi, Marco Moracci   +3 more
openaire   +1 more source