Results 51 to 60 of about 1,170 (199)
Glycosynthases: New Tools for Oligosaccharide Synthesis.
Trends in Glycoscience and Glycotechnology, 2002 生命現象におけるオリゴ糖鎖の多様な働きが次々と明らかにされている。オリゴ糖の持つ生理活性をより深く理解し利用していくためには、天然型および非天然型オリゴ糖を入手する必要がある。したがって、オリゴ糖合成は化学における最も活発に研究されている分野の一つとなっている。ここで紹介するグリコシンターゼは全く新しいタイプの変異酵素であり、有機化学的な方法や糖転移酵素を用いる従来法と共に、オリゴ糖合成に利用することができる。グリコシンターゼとは、糖加水分解酵素の触媒部位の求核性アミノ酸を非求核性アミノ酸で置換した変異体のことであり、加水分解に対して不活性である。しかし、糖受容体の存在下、糖-酵素中間体に類似した構造をもつ糖供与体に作用させると、高収率で糖転移反応を進行させることができる。本総説は ...
David L. Jakeman, Stephen G. Withers
openaire +2 more sources
Bottom‐Up Synthesized Glucan Materials: Opportunities from Applied Biocatalysis
Advanced Materials, Volume 36, Issue 27, July 4, 2024.Linear d‐glucans, simple yet versatile polysaccharides, shape nanostructures crucial for biomaterial development. Shifting from top‐down to bottom‐up synthesis using natural and engineered enzymes provides meticulous control over polymer structures, fostering diversification for macromolecular materials.
Chao Zhong, Bernd Nidetzky
wiley +1 more source
Oligosacàrids funcionals per síntesi enzimàtica. Nous enzims per a noves aplicacions [PDF]
, 2005 Davant l'interès creixent per les funcionalitats dels hidrats de carboni en l'organisme, la síntesi eficient d'oligosacàrids més complexos i polisacàrids esdevé una necessitat.
Faijes Simona, Magda, Planas, Antoni
core +1 more source
Chemistry & Biology, 2008 Glycosynthases are catalytic mutants of mainly retaining glycoside hydrolases that catalyze the synthesis of oligosaccharides from their corresponding glycosyl-fluoride donors and suitable acceptors. Here we describe the development of a general, high-throughput screening procedure for glycosynthase activity, which is based on the release of ...
Ben-David, Alon +2 more
openaire +2 more sources
Glicosidases i glicosintases en síntesi enzimàtica d’oligosacàrids: una revisió [PDF]
, 2002 Retaining glycosidases have been extensively studied for the synthesis of oligosaccharides and glycoconjugates as a convenient enzymatic approach to achieve the required regio- and stereoselectivity in glycoside bond formation as compared to chemical ...
Faijes, Magda +1 more
core
Engineering carbohydrate-active enzymes: specificity and activity remodeled [PDF]
, 2009 To understand and modify the secondary cell walls of plants the project group Enzyme Discovery in Hybrid Aspen for Fiber Engineering (EDEN) was founded composed of nine laboratories with funding from the European Commission.
Addington, Trevor
core +1 more source
EXPLORING ENDOGLYCOSIDASES FOR ANTIBODY GLYCOENGINEERING [PDF]
, 2019 Monoclonal antibodies (mAbs) comprise a rapidly growing class of therapeutics with great potential in treating infections, inflammations, cancers, and autoimmunities.
Tong, Xin
core +1 more source
Effects of Random Mutagenesis and In Vivo Selection on the Specificity and Stability of a Thermozyme [PDF]
, 2019 Factors that give enzymes stability, activity, and substrate recognition result from the combination of few weak molecular interactions, which can be difficult to study through rational protein engineering approaches.
Andrea Strazzulli +4 more
core +1 more source
Kinetic studies of a xyloglucan endotransglycosylase, a key enzyme in plant cell morphogenesis [PDF]
, 2007 El present treball de recerca s'emmarca en un projecte Europeu anomenat E.D.E.N. (Enzyme Discovery in hybrid aspen for fibre ENgineering, QLK5-CT-2001-00443), l'objectiu del qual és la identificació de nous enzims vegetals per entendre amb major ...
Saura Valls, Marc
core +1 more source
Journal of Biological Chemistry, 2012 Background: Endo-D is an endoglycosidase that can deglycosylate N-glycan core from IgG antibodies. Results: Endo-D showed distinct activities toward core-fucosylated and nonfucosylated substrates, and novel mutants were generated that demonstrated ...
Shu Fan, Wei Huang, Lai-Xi Wang
semanticscholar +1 more source