Results 31 to 40 of about 602 (161)
Expanding the Repertoire of Glycosynthases
Chemistry & Biology, 2009 As reported in this issue, Moracci and coworkers have now expanded the glycosynthase concept to two retaining alpha-L-fucosidases. The newly generated alpha-fucosynthases can use beta-L-fucosyl azide as donors for transglycosylation, enabling the synthesis of fucose-containing oligosaccharides.
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Directed Evolution of a Glycosynthase via Chemical Complementation
, 2016 Recently, we reported a general assay for enzyme catalysis based on the yeast three-hybrid assay, Chemical Complementation, which is intended to expand the range of chemical reactions to which directed evolution can be applied.
Hening Lin (1306563) +2 more
core +1 more source
Glycosynthase-catalysed formation of modified polysaccharide microstructures [PDF]
Biochemical Journal, 2004 Glycosynthases are mutant glycosidases, genetically engineered to catalyse the synthesis of oligosaccharides. A study by Faijes et al. in this issue of the Biochemical Journal has expanded the ability of glycosynthases to catalyse the polymerization of carbohydrates to form unnatural oligosaccharide products that can attain unique crystalline forms ...
Andrew G, Watts, Stephen G, Withers
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, 2005 Transglycosylation reactions (autocondensation of the substrate or transfer of the glycon donor moiety to different acceptors) with the hyperthermophilic glycosynthase from Sulfolobus solfataricus acting in dilute sodium formate buffer at pH 4.0 are ...
A Trincone +4 more
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Biocatalytic β-glucosylation/β-galactosylation of Rebaudioside C by glycosynthases
Food Materials ResearchExtracts of Stevia rebaudiana Bertoni plant leaves contain a mixture of diterpene glycosides which possess high sweetness and are used as non-caloric sweeteners in foods and beverages.
Ye-Yang Yu +7 more
doaj +1 more source
, 2016 Glycosynthases are engineered retaining glycosidases devoid of hydrolase activity that efficiently catalyze transglycosylation reactions. The mechanism of the glycosynthase reaction is probed with the E134A mutant of Bacillus licheniformis 1,3-1,4-β ...
Odette Pérez (2730778) +3 more
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Glycosynthase-Mediated Synthesis of Glycosphingolipids
, 2016 Glycosphingolipids play crucial roles in virtually every stage of the cell cycle, and their clinical administration has been proposed as a treatment for Alzheimer's, Parkinson's, stroke, and a range of other conditions.
Xiaoping Tang (234051) +5 more
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Carbohydrate-binding module assisting glycosynthase-catalysed polymerizations
Carbohydrate-binding modules (CBMs) are found within multi-modular polysaccharide degrading enzymes [glycoside hydrolases (GHs)]. CBMs play a critical role in the recognition of plant cell-wall polysaccharides and enhance the hydrolase activity of their ...
Gilbert HJ, Faijes M, Codera V, Planas A
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ChemSusChem, Volume 18, Issue 20, October 23, 2025.Developing a process to produce glycosylated natural products involves many decisions, and given the current climate crisis, the environmental impact of the various processes must be a major consideration. This review provides an overview of the various processes and a framework for which metrics should be quantified to select processes that are both ...
Felipe Mejia‐Otalvaro +3 more
wiley +1 more source
, 2016 The glycosynthase derived from Bacillus licheniformis 1,3−1,4-β-glucanase was able to polymerize glycosyl fluoride donors (G4)mG3GαF (m = 0−2, G = Glcβ) leading to artificial mixed-linked β-glucans with regular sequences and variable β1,3 to β1,4 linkage
Xavi Pérez (2231425) +2 more
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