Results 11 to 20 of about 602 (161)

Glycosynthase-Catalysed syntheses at pH below neutrality

Bioorganic & Medicinal Chemistry Letters, 2003
The use of the new glycosynthase Ta-beta-GlyE386G and of the already known Ss-beta-GlyE387G for the synthesis of interesting 4-methylumbelliferyl disaccharides and for the galactosylation of alpha- and beta-xylosides of 4-penten-1-ol is reported. The results show satisfactory yields of reaction in presence of low excesses of acceptors and demonstrated ...
Trincone A, Giordano A, Perugino G, Rossi M, Moracci M   +4 more
core   +8 more sources

A novel thermophilic Glycosynthase that effects branching glycosylation

Bioorganic & Medicinal Chemistry Letters, 2000
A novel thermophilic glycosynthase that effects branching glycosylation has been obtained by mutation of the nucleophile in the active site of the glycosidase from Sulfolobus solfataricus. Two methods for the use of this mutant are reported.
Trincone A, Perugino G, Rossi M, Moracci M   +3 more
openaire   +5 more sources

Glycosynthases in Biocatalysis

Advanced Synthesis & Catalysis, 2011
AbstractGlycosynthases, engineered glycoside hydrolases that are able to synthesize glycans in quantitative yields without hydrolyzing them, are among the most interesting tools for the chemoenzymatic synthesis of carbohydrates that have been made available so far.
Cobucci Ponzano, Beatrice, STRAZZULLI, ANDREA, ROSSI, MOSE', MORACCI, Marco   +3 more
core   +7 more sources

Chemoenzymatically Synthesized O-Acetylated GD3 Gangliosides to Examine Viral Receptor Specificities in a Cellular Context. [PDF]

Angew Chem Int Ed Engl
7‐O‐, 9‐O‐, and 7,9‐di‐O‐acetylated GD3 gangliosides can be prepared by chemical assembly of a tetrasaccharide as α‐glycosyl fluoride that is coupled to sphingosine using a glycosynthase, followed by O‐acetyl editing by coronaviral hemagglutinin‐esterases (HEs).
Zhang Z, Liang R, Hooijschuur KC, de Vries RP, Li Z, Boons GJ.   +5 more
europepmc   +3 more sources

A Fungal Versatile GH10 Endoxylanase and Its Glycosynthase Variant: Synthesis of Xylooligosaccharides and Glycosides of Bioactive Phenolic Compounds. [PDF]

Int J Mol Sci, 2022
The study of endoxylanases as catalysts to valorize hemicellulosic residues and to obtain glycosides with improved properties is a topic of great industrial interest.
Pozo-Rodríguez A, Méndez-Líter JA, de Eugenio LI, Nieto-Domínguez M, Calviño E, Cañada FJ, Santana AG, Díez J, Asensio JL, Barriuso J, Prieto A, Martínez MJ.   +11 more
europepmc   +2 more sources

Glycosynthase-based synthesis of peptidoglycan oligosaccharides to decipher bacterial cell-wall elongation processes

, 2023
International audienceMost antibiotics used in human medicine inhibit the biosynthesis of peptidoglycan, an essential component of the bacterial wall.
Richard, Emeline, Cottaz, Sylvain, Rousseau, Antoine, Simorre, Jean-Pierre, Brigandat, Louis, Ayala, Isabel, Teki, Dindet Steve-Evanes Koffi, Armand, Sylvie, Bougault, Catherine, Fort, Sébastien   +9 more
core   +7 more sources

Probing the Internalization and Efficacy of Antibody‐Drug Conjugate via Site‐Specific Fc‐Glycan Labelling of a Homogeneous Antibody Targeting SSEA‐4 Bearing Tumors

Israel Journal of Chemistry, Volume 63, Issue 10-11, October 2023., 2023
Abstract Antibody drug conjugates (ADC) are an emerging class of pharmaceuticals consisting of cytotoxic agents covalently attached to an antibody designed to target a specific cancer cell surface molecule followed by internalization and intracellular release of payload to exhibit its anticancer activity.
Vidya S Shivatare, Han‐Wen Huang, Tzu‐Hao Tseng, Po‐Kai Chuang, Yi‐Fang Zeng, Chi‐Huey Wong   +5 more
wiley   +1 more source

Design of a chimeric glycosyltransferase OleD for the site‐specific O‐monoglycosylation of 3‐hydroxypyridine in nosiheptide

Microbial Biotechnology, Volume 16, Issue 10, Page 1971-1984, October 2023., 2023
A monoglycosyl nosiheptide, which exhibit improved solubility and stability compared to nosiheptide, was created by enzymatic glycosylation. By enzyme engineering coupled with a new colorimetric method, a chimeric enzyme with approximately 70 times higher activity was constructed. These findings suggest the potential for structural modifications at the
Ling Zhao, Yuncong Xu, Manting Chen, Lingrui Wu, Meng Li, Yuanyuan Lu, Meiling Lu, Yijun Chen, Xuri Wu   +8 more
wiley   +1 more source

Glycosyl benzoates as novel substrates for glycosynthases

Organic & Biomolecular Chemistry, 2023
An engineered glycosidase from  H. orenii  showcases for the first time that glycosyl benzoates are better donors than p-nitrophenyl glucoside in enzyme-catalysed thioglucosylations. The thioglycoside products were formed with conversions up to 94%.
Sabrina de Lorenzo, Lauriane Pillet, David Lim, Francesca Paradisi   +3 more
openaire   +2 more sources

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2019–2020

Mass Spectrometry Reviews, Volume 42, Issue 5, Page 1984-2206, September/October 2023., 2023
Abstract This review is the tenth update of the original article published in 1999 on the application of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2020.
David J. Harvey
wiley   +1 more source