Synthesis of Glycosides by Glycosynthases [PDF]
Molecules, 2017 The many advances in glycoscience have more and more brought to light the crucial role of glycosides and glycoconjugates in biological processes. Their major influence on the functionality and stability of peptides, cell recognition, health and immunity ...
Marc R. Hayes, Jörg Pietruszka
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Green-Chemical Strategies for Production of Tailor-Made Chitooligosaccharides with Enhanced Biological Activities [PDF]
Molecules, 2023 Chitooligosaccharides (COSs) are b-1,4-linked homo-oligosaccharides of N-acetylglucosamine (GlcNAc) or glucosamine (GlcN), and also include hetero-oligosaccharides composed of GlcNAc and GlcN.
Reeba Thomas +2 more
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Synthesis and Characterization of a Novel Resveratrol Xylobioside Obtained Using a Mutagenic Variant of a GH10 Endoxylanase [PDF]
Antioxidants, 2022 Resveratrol is a natural polyphenol with antioxidant activity and numerous health benefits. However, in vivo application of this compound is still a challenge due to its poor aqueous solubility and rapid metabolism, which leads to an extremely low ...
Ana Pozo-Rodríguez +11 more
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Glycosylation of Epigallocatechin Gallate by Engineered Glycoside Hydrolases from Talaromyces amestolkiae: Potential Antiproliferative and Neuroprotective Effect of These Molecules [PDF]
Antioxidants, 2022 Glycoside hydrolases (GHs) are enzymes that hydrolyze glycosidic bonds, but some of them can also catalyze the synthesis of glycosides by transglycosylation.
Juan A. Méndez-Líter +9 more
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A glucotolerant β-glucosidase from the fungus Talaromyces amestolkiae and its conversion into a glycosynthase for glycosylation of phenolic compounds [PDF]
Microbial Cell Factories, 2020 Background The interest for finding novel β-glucosidases that can improve the yields to produce second-generation (2G) biofuels is still very high. One of the most desired features for these enzymes is glucose tolerance, which enables their optimal ...
Juan Antonio Méndez-Líter +8 more
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Recent Progress in Chemo-Enzymatic Methods for the Synthesis of N-Glycans [PDF]
Frontiers in Chemistry, 2020 Asparagine (N)-linked glycosylation is one of the most common co- and post-translational modifications of both intra- and extracellularly distributing proteins, which directly affects their biological functions, such as protein folding, stability and ...
Qiang Chao +5 more
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High-Throughput Recovery and Characterization of Metagenome-Derived Glycoside Hydrolase-Containing Clones as a Resource for Biocatalyst Development [PDF]
mSystems, 2019 Functional metagenomics is a powerful tool for both the discovery and development of biocatalysts. This study presents the high-throughput functional screening of 22 large-insert fosmid libraries containing over 300,000 clones sourced from natural and ...
Zachary Armstrong +8 more
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Glycosynthase Reaction Meets the Flow: Continuous Synthesis of Lacto-N-Triose II by Engineered β-Hexosaminidase Immobilized on Solid Support [PDF]
Biotechnology and Bioengineering, 2020 Juan M. Bolivar, Bernd Nidetzky
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Transglycosylation Activity of Glycosynthase Mutants of Endo-β-N-Acetylglucosaminidase from Coprinopsis cinerea. [PDF]
PLoS ONE, 2015 Endo-β-N-acetylglucosaminidase (ENGase), which catalyzes hydrolysis of N-linked oligosaccharides, is a useful tool for analyzing oligosaccharide contents of glycoproteins.
Yasunari Eshima +4 more
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Glycoengineered Monoclonal Antibodies with Homogeneous Glycan (M3, G0, G2, and A2) Using a Chemoenzymatic Approach Have Different Affinities for FcγRIIIa and Variable Antibody-Dependent Cellular Cytotoxicity Activities. [PDF]
PLoS ONE, 2015 Many therapeutic antibodies have been developed, and IgG antibodies have been extensively generated in various cell expression systems. IgG antibodies contain N-glycans at the constant region of the heavy chain (Fc domain), and their N-glycosylation ...
Masaki Kurogochi +14 more
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