Results 81 to 90 of about 2,546,109 (292)

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Management & sustainability of stockless organic arable and horticultural systems [PDF]

, 2008
The essential difference between stockless and stocked systems is that the fertility building clover ley and other “forage crops” are not processed through an animal but instead are grown as green manures which are returned directly to the soil by ...
Preston, Keith
core  

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Effects of hydrothermal temperature on kaliophilite fillers and the fire resistance of geopolymers

Journal of Materials Research and Technology
Geopolymers exhibit significant application prospect in fireproofing owing to their thermal stability. This paper adopted the kaliophilite filler prepared at different hydrothermal temperatures to further enhance the fire resistance of geopolymers, and ...
Haoxuan Zhang, Jinbang Wang, Tongsheng Zhang, Yulong Liu, Zonghui Zhou, Xiuzhi Zhang, Yongbo Huang, Xiaolei Lu, Peng Du   +8 more
doaj   +1 more source

The value of Green Star: a decade of environmental benefits [PDF]

Executive summary: Since the launch of the Green Star rating system in 2003, hundreds of buildings around Australia have been independently certified for their sustainable design and construction using Green Star rating tools.A large amount of evidence ...

core  

Nitrogen Supply and Management in Organic Farming [PDF]

, 2008
This topic review aims to summarise knowledge and experience of Nitrogen supply and management in organic farming systems, including : Nitrogen fixation; Nitrogen recycling; the effect of the length of the fertility building phase or ley and the effect ...
Briggs, Stephen
core  

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

An Ensemble Machine Learning Method for Alkali‐Activated Concrete Mix Ratio Analysis and Optimizing Experimental Results

Engineering Reports
In the field of civil engineering, the analysis of the composition of alkali‐sprayed concrete is of great significance for material research. However, there are currently no existing machine learning (ML) methods suitable for analyzing the composition of
Chengyuan Dai, Weiyu Li, Jiamin Lu, Yuelong Luo, Qizhou Liu, Lianxin Chen   +5 more
doaj   +1 more source