In vitro and in vivo validation of human and goat chondrocyte labeling by green fluorescent protein lentivirus transduction [PDF]
We investigated whether human articular chondrocytes can be labeled efficiently and for long-term with a green fluorescent protein (GFP) lentivirus and whether the viral transduction would influence cell proliferation and tissue-forming capacity.
Acharya, Chitrangada +9 more
core +1 more source
Cre Stoplight: A Red/Green Fluorescent Reporter of Cre Recombinase Expression in Living Cells
The Cre/lox system is a powerful genetic tool with which to manipulate the genome. Here, we describe the development of a simple reporter system for Cre recombinase, called the Cre Stoplight.
Yvonne S. Yang, Thomas E. Hughes
doaj +1 more source
Quantitation of mitochondrial dynamics by photolabeling of individual organelles shows that mitochondrial fusion is blocked during the Bax activation phase of apoptosis [PDF]
A dynamic balance of organelle fusion and fission regulates mitochondrial morphology. During apoptosis this balance is altered, leading to an extensive fragmentation of the mitochondria.
Arnoult, Damien +5 more
core +2 more sources
A modular toolset of phiC31-based fluorescent protein tagging vectors for Drosophila
The Drosophila transgenic technology and fluorescent protein fusions are powerful tools to analyze protein expression patterns, subcellular localization and protein dynamics.
Jun Luo, Pingping Shen, Jiong Chen
doaj +1 more source
Polyethylenimine-Modified Multiwalled Carbon Nanotubes for Plasmid DNA Gene Delivery [PDF]
An efficient molecular delivery technique based on the transporting high-molecular-weight PEI 600K-modified multiwalled carbon nanotubes (PEI 600K-MWCNTs) into cell membranes is reported.
Bo-Zhang Yu, Ji-Fei Ma, Wen-Xin Li
core +1 more source
Genetically Encoded Calcium Indicators: A New Tool in Renal Hypertension Research [PDF]
Hypertension is ranked as the third cause of disability-adjusted life-years. The percentage of the population suffering from hypertension will continue to increase over the next years.
Schleifenbaum, Johanna, Zhong, Cheng
core +1 more source
Enhanced fluorescent properties of an OmpT site deleted mutant of Green Fluorescent Protein
Background The green fluorescent protein has revolutionized many areas of cell biology and biotechnology since it is widely used in determining gene expression and for localization of protein expression. Expression of recombinant GFP in E.
Kumar Avijeet +5 more
doaj +1 more source
Murine leukemia virus (MLV) replication monitored with fluorescent proteins [PDF]
Background: Cancer gene therapy will benefit from vectors that are able to replicate in tumor tissue and cause a bystander effect. Replication-competent murine leukemia virus (MLV) has been described to have potential as cancer therapeutics, however, MLV
Bittner, Alexandra +3 more
core
Thermal green protein Q66E (TGP-E) has previously shown increased thermal stability compared to thermal green protein (TGP), a thermal stable fluorescent protein produced through consensus and surface protein engineering.
Matthew R. Anderson +3 more
doaj +1 more source
The application of fluorescent proteins as expression markers and protein fusion partners has proved immensely valuable for resolving the organization of biological events in living cells.
Aris Haryanto, Michael Kann
doaj +1 more source

