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The Role of the Protein Matrix in Green Fluorescent Protein Fluorescence
Photochemistry and Photobiology, 2006ABSTRACTIn the ground state of the highly conjugated green fluorescent protein (GFP), the chromophore should be planar. However, numerous crystal structures of GFP and GFP‐like proteins have been reported with slightly twisted chromophores. We have previously shown that the protein cavity surrounding the chromophore in wild‐type GFP is not ...
Scott L, Maddalo, Marc, Zimmer
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Photochemistry and Photobiology, 1995
Abstract— Several bioluminescent coelenterates use a secondary fluorescent protein, the green fluorescent protein (GFP), in an energy transfer reaction to produce green light. The most studied of these proteins have been the GFPs from the jellyfish Aequorea victoria and the sea pansy Renilla reniformis.
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Abstract— Several bioluminescent coelenterates use a secondary fluorescent protein, the green fluorescent protein (GFP), in an energy transfer reaction to produce green light. The most studied of these proteins have been the GFPs from the jellyfish Aequorea victoria and the sea pansy Renilla reniformis.
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Annual Review of Biochemistry, 1998
In just three years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria has vaulted from obscurity to become one of the most widely studied and exploited proteins in biochemistry and cell biology. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and ...
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In just three years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria has vaulted from obscurity to become one of the most widely studied and exploited proteins in biochemistry and cell biology. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and ...
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Reliable use of green fluorescent protein in fluorescent pseudomonads
Journal of Microbiological Methods, 2001When fluorescent pseudomonads are cultured on standard solid media under iron limiting conditions, they produce fluorescent, pigmented iron collating agents (siderophores). Siderophores can be readily identified by strong fluorescence seen under UV/blue light.
T M, Timms-Wilson, M J, Bailey
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2005
BACKGROUND. The Discovery of Green Fluorescent Protein. Photons for Reporting Molecular Events: Green Fluorescent Protein and Four Luciferase Systems. BIOCHEMISTRY AND MOLECULAR BIOLOGY OF GREEN FLUORESCENT PROTEIN. Biochemical and Physical Properties of Green Fluorescent Protein.
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BACKGROUND. The Discovery of Green Fluorescent Protein. Photons for Reporting Molecular Events: Green Fluorescent Protein and Four Luciferase Systems. BIOCHEMISTRY AND MOLECULAR BIOLOGY OF GREEN FLUORESCENT PROTEIN. Biochemical and Physical Properties of Green Fluorescent Protein.
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The conformational polymorphism of the green fluorescent protein
Molecular Biology, 2012Green fluorescent protein (GFPuv) has been widely used as a reporter fused to individual targeting sequences. However, its state in liquid and its effect on other proteins are still unclear. The conformational polymorphisms of glutathione-S-transferase-green fluorescent protein (GST-GFPuv), GFPuv and GST were analyzed by native polyacrylamide gel ...
Haidong, Tan +5 more
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Structure of cyclized green fluorescent protein
Acta Crystallographica Section D Biological Crystallography, 2002Crystals of cyclic green fluorescent protein (cGFP) engineered by the previously reported split intein technology [Iwai et al. (2001), J. Biol. Chem. 276, 16548-16554] were obtained and the structure was solved using molecular replacement. Although the core of the protein can unambiguously be fitted from the first to the last residue of the genuine ...
Hofmann, A +4 more
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The dark side of green fluorescent protein
New Phytologist, 2005Summary Here, severe interference of chlorophyll with green fluorescent protein (GFP) fluorescence is described for medicago (Medicago truncatula), rice (Oryza sativa) and arabidopsis (Arabidopsis thaliana). This interference disrupts the proportional relationship between GFP content and fluorescence that is intrinsic to its use as a quantitative ...
Xin, Zhou +3 more
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Chromophore Formation in Green Fluorescent Protein
Biochemistry, 1997The green fluorescent protein (GFP) from the jellyfish Aequorea Victoria forms an intrinsic chromophore through cyclization and oxidation of an internal tripeptide motif [Prasher, D. C., et al. (1992) Gene 111, 229-233; Cody, C. E., et al. (1993) Biochemistry 32, 1212-1218].
B G, Reid, G C, Flynn
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Charge transfer in green fluorescent protein
Photochemical & Photobiological Sciences, 2006Charge transfer reactions that contribute to the photoreactions of the wild type green fluorescent protein (GFP) do not occur in the isolated p-hydroxybenzylidene-imidazolidinone chromophore, demonstrating the role of the protein environment. The high quantum efficiency of the fluorescence photocycle that includes excited state proton transfer and the ...
Jasper J, van Thor, J Timothy, Sage
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