Results 51 to 60 of about 4,725 (112)

Fluorescent Protein Based FRET Pairs with Improved Dynamic Range for Fluorescence Lifetime Measurements.

PLoS ONE, 2015
Fluorescence Resonance Energy Transfer (FRET) using fluorescent protein variants is widely used to study biochemical processes in living cells. FRET detection by fluorescence lifetime measurements is the most direct and robust method to measure FRET. The
Bobin George Abraham, Karen S Sarkisyan, Alexander S Mishin, Ville Santala, Nikolai V Tkachenko, Matti Karp   +5 more
doaj   +1 more source

Use of Enhanced Green Fluorescent Protein to Optimize and Quantitate Infection of Target Cells with Recombinant Retroviruses

BioTechniques, 1999
Recombinant retroviral vectors are useful tools for gene transfer in both gene therapy and research applications. An enhanced form of green fluorescent protein has been incorporated into recombinant retroviruses as a marker to follow infected cells.
L.M. Cashion, L.A. Bare, S. Harvey, Q. Trinh, Y. Zhu, J.J. Devlin   +5 more
doaj   +1 more source

Three-Color Imaging Using Fluorescent Proteins in Living Zebrafish Embryos

BioTechniques, 2001
The zebrafish embryo is especially valuable for cell biological studies because of its optical clarity. In this system, use of an in vivo fluorescent reporter has been limited to green fluorescent protein (GFP).
Kenneth R. Finley, Ann E. Davidson, Stephen C. Ekker   +2 more
doaj   +1 more source

Construction of a plasmid coding for green fluorescent protein tagged cathepsin L and data on expression in colorectal carcinoma cells

Data in Brief, 2015
The endo-lysosomal cysteine cathepsin L has recently been shown to have moonlighting activities in that its unexpected nuclear localization in colorectal carcinoma cells is involved in cell cycle progression (Tamhane et al., 2015) [1]. Here, we show data
Tripti Tamhane, Brit K. Wolters, Rukshala Illukkumbura, Gunhild M. Maelandsmo, Mads H. Haugen, Klaudia Brix   +5 more
doaj   +1 more source

Green Fluorescent Protein Glows Gold [PDF]

Cell, 2008
The awarding of this year's Nobel Prize in Chemistry to Osamu Shimomura, Martin Chalfie, and Roger Tsien for their discovery and development of green fluorescent protein earns this humble jellyfish protein a place of honor in the biology research hall of fame.
openaire   +2 more sources

Differentiation of Apical Bud Cells in a Newly Developed Apical Bud Transplantation Model Using GFP Transgenic Mice as Donor.

PLoS ONE, 2016
Rodent mandibular incisors have a unique anatomical structure that allows teeth to grow throughout the lifetime of the rodent. This report presents a novel transplantation technique for studying the apical bud differentiation of rodent mandibular ...
Naoki Maruo, Ryuji Sakagami, Yasunori Yoshinaga, Kazuhiko Okamura, Yoshihiko Sawa   +4 more
doaj   +1 more source

Green Fluorescent Protein- and Discosoma sp. Red Fluorescent Protein-Tagged Organelle Marker Lines for Protein Subcellular Localization in Rice

Frontiers in Plant Science, 2019
The subcellular localization of proteins is a fundamental aspect of protein functions. Determining the subcellular localization is important for understanding the biological functions of proteins.
Ziqiang Chen, Wenhui Zheng, Wenhui Zheng, Longhai Chen, Chenlu Li, Tingmin Liang, Zaijie Chen, Huibing Xu, Yijuan Han, Lan Kong, Xu Zhao, Feng Wang, Zonghua Wang, Zonghua Wang, Songbiao Chen   +14 more
doaj   +1 more source

Fluorescent leukotriene B4: potential applications

Journal of Lipid Research, 2005
Leukotriene B4 (LTB4) is a potent lipid mediator of inflammation that acts primarily via a seven-transmembrane-spanning, G-protein-coupled receptor denoted BLT1. Here, we describe the synthesis and characterization of fluorescent analogs of LTB4 that are
Alan Sabirsh, Anders Wetterholm, Jesper Bristulf, Hakon Leffler, Jesper Z. Haeggström, Christer Owman   +5 more
doaj   +1 more source

Cre Stoplight: A Red/Green Fluorescent Reporter of Cre Recombinase Expression in Living Cells

BioTechniques, 2001
The Cre/lox system is a powerful genetic tool with which to manipulate the genome. Here, we describe the development of a simple reporter system for Cre recombinase, called the Cre Stoplight.
Yvonne S. Yang, Thomas E. Hughes
doaj   +1 more source

A modular toolset of phiC31-based fluorescent protein tagging vectors for Drosophila

Fly, 2019
The Drosophila transgenic technology and fluorescent protein fusions are powerful tools to analyze protein expression patterns, subcellular localization and protein dynamics.
Jun Luo, Pingping Shen, Jiong Chen
doaj   +1 more source