Results 61 to 70 of about 4,725 (112)

Nuclear Import Analysis of Two Different Fluorescent Marker Proteins into Hepatocyte Cell Lines (HuH-7 Cell)

open access: yesIndonesian Journal of Biotechnology, 2005
The application of fluorescent proteins as expression markers and protein fusion partners has proved immensely valuable for resolving the organization of biological events in living cells.
Aris Haryanto, Michael Kann
doaj   +1 more source

The Crystal Structure of Thermal Green Protein Q66E (TGP-E) and Yellow Thermostable Protein (YTP-E) E148D

open access: yesSynBio
Thermal green protein Q66E (TGP-E) has previously shown increased thermal stability compared to thermal green protein (TGP), a thermal stable fluorescent protein produced through consensus and surface protein engineering.
Matthew R. Anderson   +3 more
doaj   +1 more source

Enhanced fluorescent properties of an OmpT site deleted mutant of Green Fluorescent Protein

open access: yesMicrobial Cell Factories, 2010
Background The green fluorescent protein has revolutionized many areas of cell biology and biotechnology since it is widely used in determining gene expression and for localization of protein expression. Expression of recombinant GFP in E.
Kumar Avijeet   +5 more
doaj   +1 more source

Selective Immobilization of Fluorescent Proteins for the Fabrication of Photoactive Materials

open access: yesMolecules, 2019
The immobilization of fluorescent proteins is a key technology enabling to fabricate a new generation of photoactive materials with potential technological applications. Herein we have exploited superfolder green (sGFP) and red (RFP) fluorescent proteins
Ana I. Benítez-Mateos   +5 more
doaj   +1 more source

Molecular mechanism of a green-shifted, pH-dependent red fluorescent protein mKate variant.

open access: yesPLoS ONE, 2011
Fluorescent proteins that can switch between distinct colors have contributed significantly to modern biomedical imaging technologies and molecular cell biology.
Qi Wang   +9 more
doaj   +1 more source

Green fluorescent protein inspired fluorophores

Advances in Colloid and Interface Science, 2020
Green fluorescence proteins (GFP) are appealing to a variety of biomedical and biotechnology applications, such as protein fusion, subcellular localizations, cell visualization, protein-protein interaction, and genetically encoded sensors. To mimic the fluorescence of GFP, various compounds, such as GFP chromophores analogs, hydrogen bond-rich proteins,
Jia Kong, Yuefei Wang, Renliang Huang
exaly   +3 more sources

Structure and dynamics of green fluorescent protein

Current Opinion in Structural Biology, 1997
Many marine organisms are luminescent. The proteins that produce the light include a primary light producer (aequorin or luciferase) and often a secondary photoprotein that red shifts the light for better penetration in the ocean. Green fluorescent protein is one such secondary protein. It is remarkable in that it autocatalyzes the formation of its own
George N Phillips
exaly   +3 more sources

Deconstructing Green Fluorescent Protein

Journal of the American Chemical Society, 2008
Green fluorescent protein (GFP) has been reassembled from two pieces, a large fragment 214 amino acids in length that is produced recombinantly (GFP 1-10) and a short synthetic peptide corresponding to the 11th stave of the beta-barrel that is 16 amino acids long (synthetic GFP 11), following a system developed by Waldo and co-workers (Cabantous, S ...
Kevin P, Kent   +2 more
openaire   +2 more sources

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