Results 41 to 50 of about 4,725 (112)

Time course analysis of sensory axon regeneration in vivo by directly tracing regenerating axons

Neural Regeneration Research, 2020
Most current studies quantify axon regeneration by immunostaining regeneration-associated proteins, representing indirect measurement of axon lengths from both sensory neurons in the dorsal root ganglia and motor neurons in the spinal cord.
Yan Gao, Yi-Wen Hu, Run-Shan Duan, Shu-Guang Yang, Feng-Quan Zhou, Rui-Ying Wang   +5 more
doaj   +1 more source

A Bright, Nontoxic, and Non-aggregating red Fluorescent Protein for Long-Term Labeling of Fine Structures in Neurons

Frontiers in Cell and Developmental Biology, 2022
Red fluorescent proteins are useful as morphological markers in neurons, often complementing green fluorescent protein-based probes of neuronal activity. However, commonly used red fluorescent proteins show aggregation and toxicity in neurons or are dim.
Lin Ning, Yang Geng, Yang Geng, Matthew Lovett-Barron, Xiaoman Niu, Mengying Deng, Liang Wang, Niloufar Ataie, Alex Sens, Ho-Leung Ng, Shoudeng Chen, Karl Deisseroth, Michael Z. Lin, Michael Z. Lin, Jun Chu   +14 more
doaj   +1 more source

Application of a Chimeric Green Fluorescent Protein to Study Protein-Protein Interactions

BioTechniques, 1997
The green fluorescent protein (GFP) of the jellyfish Aequorea victoria is an emerging tool to monitor gene expression in situ and in vivo. Because of its fluorescence properties, when GFP is fused in-frame to a specific protein of interest, various ...
N. Garamszegi, Z.P. Garamszegi, M.S. Rogers, S.J. De-Marco, E.E. Strehler   +4 more
doaj   +1 more source

A super-ecliptic, pHluorin-mKate2, tandem fluorescent protein-tagged human LC3 for the monitoring of mammalian autophagy.

PLoS ONE, 2014
Tandem fluorescent protein-tagged LC3s that were comprised of a protein tag that emits green fluorescence (e.g., EGFP or mWasabi) fused with another tag that emits red fluorescence (e.g.
Isei Tanida, Takashi Ueno, Yasuo Uchiyama   +2 more
doaj   +1 more source

Fluorescent Labeling of Peroxisome and Nuclear in Colletotrichum aenigma

Journal of Fungi, 2023
Anthracnose is one of the most widespread and destructive diseases in grapes. Grape anthracnose can be caused by various Colletotrichum species, such as Colletotrichum gloeosporioides and Colletotrichum cuspidosporium.
Shendan Yu, Jing Wang, Rongyao Chai, Haiping Qiu, Ziqi Lu, Zhen Zhang, Lin Li, Jiaoyu Wang, Guochang Sun   +8 more
doaj   +1 more source

Recombinant Bombyx mori Nucleopolyhedrovirus Harboring Green Fluorescent Protein

BioTechniques, 1998
The gene encoding the green fluorescent protein (gfp) under the control of the highly expressed Autographa californica nucleopolyhedrovirus (AcMNPV)-polyhedrin promoter has been introduced into the polyhedron (polh) locus of Bombyx mori ...
Deepak Sehgal, Karumathil P. Gopinathan
doaj   +1 more source

Subatomic resolution X-ray structures of green fluorescent protein

IUCrJ, 2019
Green fluorescent protein (GFP) is a light-emitting protein that does not require a prosthetic group for its fluorescent activity. As such, GFP has become indispensable as a molecular tool in molecular biology.
Kiyofumi Takaba, Yang Tai, Haruhiko Eki, Hoang-Anh Dao, Yuya Hanazono, Kazuya Hasegawa, Kunio Miki, Kazuki Takeda   +7 more
doaj   +1 more source

Synthetic Control of Green Fluorescent Protein [PDF]

Journal of the American Chemical Society, 2009
Semisynthetic green fluorescent proteins (GFPs) can be prepared by producing truncated GFPs recombinantly and assembling them with synthetic beta-strands of GFP. The yield from expressing the truncated GFPs is low, and the chromophore is either partially formed or not formed. An alternative method is presented in which full-length proteins are produced
Kevin P, Kent, Luke M, Oltrogge, Steven G, Boxer   +2 more
openaire   +2 more sources

Parasite Burden Measurement in the Leishmania major Infected Mice by Using the Direct Fluorescent Microscopy, Limiting Dilution Assay, and Real-Time PCR Analysis

Iranian Journal of Parasitology, 2020
Background: We aimed to compare parasite burden in BALB/c mice, using three methods including the direct fluorescent microscopic using recombinant Leishmania major expressing an enhanced green fluorescent protein, limiting dilution assay, and real-time ...
Sepideh HAGHDOUST, Mahdieh AZIZI, Mostafa HAJI MOLLA HOSEINI, Mojgan BANDEHPOUR, Mandana MOHSENI MASOOLEH, Farshid YEGANEH   +5 more
doaj   +1 more source

Proton Pathways in Green Fluorescence Protein [PDF]

Biophysical Journal, 2005
Proton pathways in green fluorescent protein (GFP) are more extended than previously reported. In the x-ray data of wild-type GFP, a two-step exit pathway exists from the active site to the protein surface, controlled by a threonine switch. A proton entry pathway begins at a glutamate-lysine cluster around Glu-5, and extends all the way to the buried ...
openaire   +2 more sources