Results 21 to 30 of about 4,725 (112)

Use of Green Fluorescent Protein to Detect Expression of nif Genes of Azoarcus sp. BH72, a Grass-Associated Diazotroph, on Rice Roots

Molecular Plant-Microbe Interactions, 1998
A gfp (green fluorescent protein) cassette for transcriptional fusions has been developed to study gene expression in Azoarcus sp. BH72 in association with plant roots.
Tanja Egener, Thomas Hurek, Barbara Reinhold-Hurek   +2 more
doaj   +1 more source

Histo-pathology study of the growth of Trichoderma harzianum, Phaeomoniella chlamydospora and Eutypa lata on grapevine pruning wounds

Phytopathologia Mediterranea, 2011
Protecting grapevine pruning wounds by inoculating them with Trichoderma spp. can preventinfection from trunk disease pathogens. The growth and interactions of both, the biological control agent Trichoderma spp.
Cheusi MUTAWILA, Paul FOURIE, Francois HALLEEN, Lizel MOSTERT   +3 more
doaj   +1 more source

Construction of Yersinia pestis Strain Producing Fluorescent Protein GFP and Prospects of Its Usage

Проблемы особо опасных инфекций, 2020
Objective of the study was to construct the recombinant Y. pestis strain producing fluorescent protein GFP and analysis of the prospects of its usage for investigation of interaction of the agent with the protozoa, and also macroorganism of mammals ...
L. M. Kukleva, I. V. Tuchkov, E. G. Oglodin, Z. L. Devdariani, O. A. Morozov, O. S. Kuznetsov, V. G. Germanchuk, G. A. Eroshenko   +7 more
doaj   +1 more source

Simultaneous Real-Time Detection of Initiator- and Effector-Caspase Activation by Double Fluorescence Resonance Energy Transfer Analysis

Journal of Pharmacological Sciences, 2005
Fluorescence resonance energy transfer (FRET) with green fluorescent protein (GFP) variants has become widely used for biochemical research. In order to expand the choice of fluorescent range in FRET analysis, we designed various color versions of the ...
Hiroshi Kawai, Takuo Suzuki, Tetsu Kobayashi, Haruna Sakurai, Hisayuki Ohata, Kazuo Honda, Kazutaka Momose, Iyuki Namekata, Hikaru Tanaka, Koki Shigenobu, Ryu Nakamura, Takao Hayakawa, Toru Kawanishi   +12 more
doaj   +1 more source

Implementation of a Practical Teaching Course on Protein Engineering

Biology, 2022
Protein Engineering is a highly evolved field of engineering aimed at developing proteins for specific industrial, medical, and research applications.
Luciana C. Gomes, Carla Ferreira, Filipe J. Mergulhão   +2 more
doaj   +1 more source

Herpes Simplex Virus Thymidine Kinase-Green Fluorescent Protein Fusion Gene: New Tool for Gene Transfer Studies and Gene Therapy

BioTechniques, 1998
Green fluorescent protein (GFP) and herpes simplex virus type-1 thymidine kinase (TK) are commonly used markers in gene transfer studies. The latter gene has also proven to be an effective tool in cancer “suicide” gene therapy.
Sami Loimas, Jarmo Wahlfors, Juhani Jänne   +2 more
doaj   +1 more source

Evolution of a fluorinated green fluorescent protein [PDF]

Proceedings of the National Academy of Sciences, 2007
The fluorescence of bacterial cells expressing a variant (GFPm) of the green fluorescent protein (GFP) was reduced to background levels by global replacement of the leucine residues of GFPm by 5,5,5-trifluoroleucine. Eleven rounds of random mutagenesis and screening via fluorescence-activated cell sorting yielded a GFP mutant containing 20
Yoo, Tae-Hyeon, Link, A. James, Tirrell, David A.   +2 more
openaire   +3 more sources

Generation of a human embryonic stem cell line expressing tetrameric Zoanthus sp. green fluorescent protein: NERCe002-A-1

Stem Cell Research, 2018
The human embryonic stem cell (hESC) line NERCe002-A-1 was generated through lentiviral transduction of the original NERCe002-A-1 hESC line with Zoanthus sp. green fluorescent protein (ZsGreen). Cells that expressed ZsGreen showed a >8.6-fold increase in
Xingxiang Duan, Menghan Xie, Yingying Peng, Liang Hu, Juan Yu, Sicong Zeng, Yang Wang, Guangxiu Lu, Ge Lin, Yi Sun   +9 more
doaj   +1 more source

Development and Applications of Enhanced Green Fluorescent Protein Mutants

BioTechniques, 1998
The introduction of several mutations resulted in the generation of improved mutants of the green fluorescent protein (GFP). A strong green (GFPsg25) and blue (BFPsg50) fluorescent protein, gave 50-fold–100-fold brighter fluorescence compared to wild ...
R.H. Stauber, K. Horie, P. Carney, E.A. Hudson, N.I. Tarasova, G.A. Gaitanaris, G.N. Pavlakis   +6 more
doaj   +1 more source

Fluorophores for live cell imaging of AGT fusion proteins across the visible spectrum

BioTechniques, 2006
O6-alkylguanine-DNA alkyltransferase (AGT) fusion proteins can be specifically and covalently labeled with fluorescent O6-benzylguanine (O6-BG) derivatives for multicolor live cell imaging approaches.
Antje Keppler, Claudio Arrivoli, Lucia Sironi, Jan Ellenberg   +3 more
doaj   +1 more source