Results 11 to 20 of about 4,725 (112)

Origin of the Intrinsic Fluorescence of the Green Fluorescent Protein [PDF]

Journal of the American Chemical Society, 2017
Green fluorescent protein, GFP, has revolutionized biology, due to its use in bioimaging. It is widely accepted that the protein environment makes its chromophore fluoresce, whereas the fluorescence is completely lost when the native chromophore is taken out of GFP.
Annette Svendsen, Hjalte V. Kiefer, Henrik B. Pedersen, Anastasia V. Bochenkova, Lars H. Andersen   +4 more
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Photochromicity and Fluorescence Lifetimes of Green Fluorescent Protein [PDF]

The Journal of Physical Chemistry B, 1999
The green fluorescent protein (GFP) of the bioluminescent jellyfish Aequorea and its mutants have gained widespread usage as an indicator of structure and function within cells. Proton transfer has been implicated in the complex photophysics of the wild-type molecule, exhibiting a protonated A species excited at 400 nm, and two deprotonated excited ...
Striker, G., Subramaniam, V., Seidel, C., Volkmer, A.   +3 more
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Imaging the Environment of Green Fluorescent Protein [PDF]

Biophysical Journal, 2002
An emerging theme in cell biology is that cell surface receptors need to be considered as part of supramolecular complexes of proteins and lipids facilitating specific receptor conformations and distinct distributions, e.g., at the immunological synapse. Thus, a new goal is to develop bioimaging that not only locates proteins in live cells but can also
Suhling, K, Siegel, J, Phillips, D, Davis, D M, LevequeFort, S, Webb, S E D, French, P M W   +6 more
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Green Fluorescent Protein-Mediated Biomineralization of Silver Microparticles

Orbital: The Electronic Journal of Chemistry, 2021
Biomineralization is a bio-inspired technique of creating inorganic nanostructures using peptides or proteins. An important consideration in selecting a biomineralization agent is the overall shape or geometry of the protein since this can influence the
Ma. Monica M. Cabiles, Brandon Cyril S. Lira, Jose Isagani B. Janairo   +2 more
doaj   +3 more sources

Using photoactivatable fluorescent protein Dendra2 to track protein movement

BioTechniques, 2007
Photoactivatable fluorescent proteins are capable of dramatic changes in fluorescent properties in response to specific light irradiation. For example, they can be converted from cyan to green, or from green to red, or from nonfluorescent to a brightly ...
Dmitriy M. Chudakov, Sergey Lukyanov, Konstantin A. Lukyanov   +2 more
doaj   +1 more source

Two-Color Green Fluorescent Protein Time-Lapse Imaging

BioTechniques, 1998
The Aequorea victoria green fluorescent protein (GFP) is widely recognized as a powerful tool in cell biology, serving as a vital reporter for monitoring localization and dynamics of intracellular proteins and organelles over time.
Jan Ellenberg, Jennifer Lippincott-Schwartz, John F. Presley   +2 more
doaj   +1 more source

Green fluorescent protein: A perspective [PDF]

Protein Science, 2011
AbstractA brief personal perspective is provided for green fluorescent protein (GFP), covering the period 1994–2011. The topics discussed are primarily those in which my research group has made a contribution and include structure and function of the GFP polypeptide, the mechanism of fluorescence emission, excited state protein transfer, the design of ...
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Aequorea's secrets revealed: New fluorescent proteins with unique properties for bioimaging and biosensing.

PLoS Biology, 2020
Using mRNA sequencing and de novo transcriptome assembly, we identified, cloned, and characterized 9 previously undiscovered fluorescent protein (FP) homologs from Aequorea victoria and a related Aequorea species, with most sequences highly divergent ...
Gerard G Lambert, Hadrien Depernet, Guillaume Gotthard, Darrin T Schultz, Isabelle Navizet, Talley Lambert, Stephen R Adams, Albertina Torreblanca-Zanca, Meihua Chu, Daphne S Bindels, Vincent Levesque, Jennifer Nero Moffatt, Anya Salih, Antoine Royant, Nathan C Shaner   +14 more
doaj   +1 more source

Red fluorescent proteins engineered from green fluorescent proteins

Proceedings of the National Academy of Sciences, 2023
Fluorescent proteins (FPs) form a fluorophore through autocatalysis from three consecutive amino acid residues within a polypeptide chain. The two major groups, green FPs (GFPs) and red FPs (RFPs), have distinct fluorophore structures; RFPs have an extended π-conjugation system with an additional double bond.
Hiromi Imamura, Shiho Otsubo, Mizuho Nishida, Norihiro Takekawa, Katsumi Imada   +4 more
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Effects of cellular prion protein on rapid eye movement sleep deprivation-induced spatial memory impairment

Journal of Integrative Neuroscience, 2019
The effects of cellular prion protein on rapid eye movement sleep deprivation-induced spatial memory impairment were investigated, and the related mechanisms explored.
Li Hu, Peng Li, Zhendong You, Zhaohuan Zhang, Hongyang Jin, Liuqing Huang   +5 more
doaj   +1 more source