Results 71 to 80 of about 910,722 (206)
Subatomic resolution X-ray structures of green fluorescent protein
IUCrJ, 2019 Green fluorescent protein (GFP) is a light-emitting protein that does not require a prosthetic group for its fluorescent activity. As such, GFP has become indispensable as a molecular tool in molecular biology.
Kiyofumi Takaba +7 more
doaj +1 more source
Iranian Journal of Parasitology, 2020 Background: We aimed to compare parasite burden in BALB/c mice, using three methods including the direct fluorescent microscopic using recombinant Leishmania major expressing an enhanced green fluorescent protein, limiting dilution assay, and real-time ...
Sepideh HAGHDOUST +5 more
doaj +1 more source
Protein Mobility in the Cytoplasm of Escherichia coli [PDF]
, 1999 The rate of protein diffusion in bacterial cytoplasm may constrain a variety of cellular functions and limit the rates of many biochemical reactions in vivo. In this paper, we report noninvasive measurements of the apparent diffusion coefficient of green
Elowitz, Michael B. +4 more
core +2 more sources
A faster way to make GFP-based biosensors: Two new transposons for creating multicolored libraries of fluorescent fusion proteins [PDF]
, 2004 Background There are now several ways to generate fluorescent fusion proteins by randomly inserting DNA encoding the Green Fluorescent Protein (GFP) into another protein's coding sequence. These approaches can be used to map regions in a protein that are
Douglas L Sheridan, Thomas E Hughes
core +2 more sources
PLoS ONE, 2015 Fluorescence Resonance Energy Transfer (FRET) using fluorescent protein variants is widely used to study biochemical processes in living cells. FRET detection by fluorescence lifetime measurements is the most direct and robust method to measure FRET. The
Bobin George Abraham +5 more
doaj +1 more source
Synthetic Control of Green Fluorescent Protein [PDF]
Journal of the American Chemical Society, 2009 Semisynthetic green fluorescent proteins (GFPs) can be prepared by producing truncated GFPs recombinantly and assembling them with synthetic beta-strands of GFP. The yield from expressing the truncated GFPs is low, and the chromophore is either partially formed or not formed. An alternative method is presented in which full-length proteins are produced
Kevin P, Kent +2 more
openaire +2 more sources
BioTechniques, 1999 Recombinant retroviral vectors are useful tools for gene transfer in both gene therapy and research applications. An enhanced form of green fluorescent protein has been incorporated into recombinant retroviruses as a marker to follow infected cells.
L.M. Cashion +5 more
doaj +1 more source
Development of a method for screening short-lived proteins using green fluorescent protein. [PDF]
, 2004 We have developed a screening technology for the identification of short-lived proteins. A green fluorescent protein (GFP)-fusion cDNA library was generated for monitoring degradation kinetics. Cells expressing a subset of the GFP-cDNA expression library
Coffino, Philip +2 more
core
A comparison of enhancement techniques for footwear impressions on dark and patterned fabrics [PDF]
, 2013 The use of chemical enhancement techniques on porous substrates, such as fabrics, poses several challenges predominantly due to the occurrence of background staining and diffusion as well as visualisation difficulties.
Bandey, Helen +3 more
core +3 more sources
Three-Color Imaging Using Fluorescent Proteins in Living Zebrafish Embryos
BioTechniques, 2001 The zebrafish embryo is especially valuable for cell biological studies because of its optical clarity. In this system, use of an in vivo fluorescent reporter has been limited to green fluorescent protein (GFP).
Kenneth R. Finley +2 more
doaj +1 more source