Results 211 to 220 of about 60,004 (331)

CRISPR/Cas9‐mediated mutagenesis of the white‐eye gene in the tephritid pest Bactrocera zonata

Insect Science, EarlyView.
Abstract Bactrocera zonata is a highly invasive agricultural pest that causes extensive damage to fruit crops. The Sterile Insect Technique (SIT), a species‐specific and environmentally friendly pest control method, significantly benefits from the availability of Genetic Sexing Strains (GSSs) that enable efficient mass production of males for sterile ...
Albert Nazarov, Tamir Partosh, Flavia Krsticevic, Dimitris Rallis, Yael Arien, Guy Ostrovsky, Reut Madar Kramer, Eyal Halon, Alfred M. Handler, Simon W. Baxter, Yoav Gazit, Kostas D. Mathiopoulos, Gur Pines, Philippos A. Papathanos   +13 more
wiley   +1 more source

Sequence and expression analysis of potential spermatogenesis‐specific gene cognates in the Caribbean fruit fly, Anastrepha suspensa

Insect Science, EarlyView.
Abstract The sterile insect technique (SIT) is a highly effective biologically‐based method for the suppression of many insect pest populations. SIT efficacy could be improved by methods of male sterilization that avoid the use of irradiation that can result in diminished fitness and mating competitiveness.
Alfred M. Handler, Richard B. Furlong, Chao Chen, Daniel A. Hahn   +3 more
wiley   +1 more source

A genome-wide MAGIC kit for recombinase-independent mosaic analysis in <i>Drosophila</i>. [PDF]

Elife
Shen Y, Yeung AT, Ditchfield P, Korn E, Clements R, Chen X, Wang B, Huang Z, Sheen M, Jarman PA, Han C.   +10 more
europepmc   +1 more source

CRISPR/Cas9‐based white pupae mutant lines in Bactrocera spp. for sterile insect technique applications

Insect Science, EarlyView.
Bactrocera fruit flies are significant horticultural pests that cause major economic losses. A “neoclassical approach” incorporating genome editing via CRISPR/Cas9 to develop genetic sexing strains (GSS) could render the sterile insect technique (SIT) against these pests more efficient and cost‐effective.
Chrysanthi Ioannidou, Maria‐Eleni Gregoriou, Marc F. Schetelig, Elena Drosopoulou, Kostas D. Mathiopoulos, Kostas Bourtzis   +5 more
wiley   +1 more source

CRISPR/Cas9‐mediated Genome‐editing Reveals 10 Testis‐enriched Genes and One Non‐testis‐enriched Gene are Dispensable for Male Fecundity in Mice

Andrology, EarlyView.
ABSTRACT Background More than 1000 genes have been identified as predominantly expressed in the human testis. Advances in gene editing technologies have enabled the rapid and efficient generation of genetically engineered mice. This approach facilitates the screening of genes essential for spermatogenesis by analyzing knockout mouse models.
Yumiao Qiu, Keisuke Shimada, Masahito Ikawa   +2 more
wiley   +1 more source

Microtubule Inner Protein CFAP77 Contributes to Sperm Motility and Male Fertility in Mice

Andrology, EarlyView.
ABSTRACT Background Sperm motility is essential for male fertility, and its regulation is dependent on the structural integrity of the axoneme. The axoneme consists of a conserved “9+2” microtubule arrangement and is supported by microtubule inner proteins. However, the functional significance of many microtubule inner proteins remains unclear.
Haoting Wang, Anh Hoang Pham, Mengjiao Luo, Masahito Ikawa, Haruhiko Miyata   +4 more
wiley   +1 more source

Stunning Intricacies of RNA Editing Complexes RECC, RESC, and REH2C: Functional Organization, Developmental Regulation, and Evolutionary History in Kinetoplastid Protists. [PDF]

Wiley Interdiscip Rev RNA
McDermott SM, Lukeš J, Read LK, Salavati R, Schnaufer A, Zimmer SL, Carnes J, Ivens A, Poorinmohammad N, Savill NJ, Speijer D, Stuart K, Záhonová K, Borujeni PM, Chen Z, Goode C, Sharma SK, O'Hara L, Cruz-Reyes J.   +18 more
europepmc   +1 more source

In‐Tumor CRISPR‐Cas9 Knockout Screening and Novel Therapy Development for Malignant Transformation of Ovarian Teratoma

Cancer Science, EarlyView.
We conducted an in vivo genome‐wide CRISPR‐Cas9 knockout screen using MTMCT‐derived cells to identify genes essential for tumor growth. SOD1 emerged as a key therapeutic target, and its inhibition suppressed tumor proliferation by disrupting redox homeostasis, both in vitro and in PDX models. ABSTRACT Malignant transformation of mature cystic teratoma (
Satoshi Tamauchi, Kosuke Yoshida, Wang Xinyuan, Atsushi Nakagawa, Akira Yokoi, Nobuhisa Yoshikawa, Kaoru Niimi, Yusuke Yamamoto, Hiroaki Kajiyama   +8 more
wiley   +1 more source

Enhancing KLF15 activity in cardiomyocytes: a novel approach to prevent pathological reprogramming and fibrosis via nuclease-deficient dCas9VPR. [PDF]

Signal Transduct Target Ther
Schoger E, Kim R, Bleckwedel F, Peralta T, Priesmeier L, Fischer J, Stengel L, Rocha C, Santos GL, Lutz S, Boileau E, Baumgarten N, Schulz MH, Dieterich C, Müller OJ, Cyganek L, Cabrera-Orefice A, Eberl H, Maack C, Streckfuss-Bömeke K, Pavez-Giani M, Doroudgar S, Sossalla ST, Zelarayán LC.   +23 more
europepmc   +1 more source

Zebrafish as a model for Catel–Manzke syndrome—identification and characterization of the zebrafish TGDS ortholog

The FEBS Journal, EarlyView.
Zebrafish Tgds, when expressed as a recombinant protein, catalyzes the dehydration of UDP‐D‐glucose, the initial step in the formation of 6‐deoxyhexoses. Corresponding Tgds mutations found in Catel–Manzke syndrome patients lead to reduced enzymatic activity and stability.
Maria Rosaria Coppola, Deianira Bellitto, Erfan Asgari, Virginia Bazzurro, Giorgia Casucci, Francesco Piacente, Matteo Bozzo, Davide Ceresa, Costantino Parisi, Cecilia Winata, Simona Candiani, Michela Tonetti   +11 more
wiley   +1 more source