Results 231 to 240 of about 60,004 (331)

Coupling of both a transactivation module and a double‐stranded DNA‐binding domain boosts Cas12i3 variant‐based cytosine and adenine editing in plants

Journal of Integrative Plant Biology, EarlyView.
Fusions of a transactivation module and a double‐stranded DNA‐binding domain produced effective Cas12i3 variant‐based cytosine and adenine base editors, with improved C‐to‐T and A‐to‐G base editing efficiency and expanded editing window. Generated herbicide‐resistant rice germplasm by using these base editors demonstrates their potential for precise ...
Chen Zhang, Jingying Li, Yucai Li, Lei Yan, Christina Seok Yien Yong, Shaoya Li, Yubing He, Lanqin Xia   +7 more
wiley   +1 more source

In Silico Design and Characterization of a Rationally Engineered Cas12j2 Gene Editing System for the Treatment of HPV-Associated Cancers. [PDF]

Int J Mol Sci
Boren C, Kumar R, Gollahon L.
europepmc   +1 more source

Enhanced exonuclease–Cas9 systems promote multiple nucleotide deletions with higher efficiency and broader targeting scope in plants

Journal of Integrative Plant Biology, EarlyView.
Cas9 preferentially generates small indels. By tethering an exonuclease and a DNA‐binding domain (DBD) to Cas9, the Exo‐DBD‐Cas9 system efficiently promotes multiple nucleotide deletions. These expanded deletions enable effective disruption of miRNA‐mediated regulation and relieve translational repression through targeted editing of 3′ untranslated ...
Rui Zhang, Xu Tang, Yao He, Wei Wang, Qiurong Ren, Yiping Qi, Yong Zhang   +6 more
wiley   +1 more source

In Silico Design of gRNA for CRISPR System for Detection of Multidrug Resistant Tuberculosis Using Indian Mycobacterium tuberculosis Genomes: A Computational Study. [PDF]

Cureus
Mittal A, Manna S, Nelson V, Ladha N.
europepmc   +1 more source

The transcription factors TaABI4 and TaNAC‐A1 fine‐tune TaCYP94‐A1 transcription to modulate seed dormancy in wheat

Journal of Integrative Plant Biology, EarlyView.
A module involving the transcription factors TaABI4 and TaNAC‐A1, and the cytochrome P450 TaCYP94‐A1 precisely regulates seed dormancy levels through plant hormone crosstalk, providing targets for improving resistance to pre‐harvest sprouting in wheat.
Litian Zhang, Yuhui Fang, Yunfei Zhou, Xianlai Nie, Shujun Cao, Wei Gao, Yu Wang, Lianghua Dai, Lin Ye, Jie Lu, Chuanxi Ma, Cheng Chang, Haiping Zhang   +12 more
wiley   +1 more source

Reactivation of the silenced <i>BASP1</i> gene suppresses oncogenic WNT signaling in human colorectal cancer cells. [PDF]

Proc Natl Acad Sci U S A
Weber LI, Timpen LE, Egger-Hörschinger AS, Schöpf P, Ayhan ND, Demmel D, Hotze M, Zhang Y, Mehrabi M, Puglisi K, Stefan E, Ghaffari-Tabrizi-Wizsy N, Ramos-Pittol JM, Kwiatkowski M, Hartl M.   +14 more
europepmc   +1 more source

A stable but reversible integrated surrogate reporter for assaying CRISPR/Cas9-stimulated homology-directed repair [PDF]

, 2017
Blanc, Valerie, Connelly, Jon P, Davidson, Nicholas O, Liao, Grace, Madison, Blair B, Pritchard, Thomas, Pruett-Miller, Shondra M, Wen, Yahong, Zhao, Ting-Ting   +8 more
core   +2 more sources

A leucine‐rich‐repeat receptor‐like kinase SERL1 phosphorylates and stabilizes OsALDH2B1 to promote alkaline tolerance and grain size in rice

Journal of Integrative Plant Biology, EarlyView.
The rice aldehyde dehydrogenase OsALDH2B1 enhances grain size and tolerance to alkaline soil by repressing GRAIN SIZE 3 and activating catalases. SERL1‐mediated phosphorylation stabilizes OsALDH2B1 under stress, establishing a signaling axis that overcomes growth‐defense trade‐offs and provides a direct target for breeding high‐yield, alkaline ...
Zemin Ma, Xuanlin Gao, Shuaizu An, Mengyuan Chen, Biaoming Zhang, Lei Zhou, Shengyuan Sun, Ming Li, Feng Yu, Shiyou Lü, Zhaohui Chu, Aiqing You, Yinggen Ke, Pingfang Yang   +13 more
wiley   +1 more source

Comprehensive profiling of CRISPR/dCas9 epigenome editors indicates a complex link between on and off target effects. [PDF]

Genome Biol
Pahlevan Kakhki M, Rangani F, Ewing E, Starvaggi Cucuzza C, Zheleznyakova G, Kalomoiri M, Kenny L, Raghavan A, Rao Prakash C, van den Hoeven G, Venkata S Badam T, Covacu R, Andreou I, Needhamsen M, Kular L, Jagodic M.   +15 more
europepmc   +1 more source

OsCPN10a, cooperating with OsCPN20 and OsHSP60‐3B negatively regulate ABA signaling and enhance seed storability in rice

Journal of Integrative Plant Biology, EarlyView.
Abscisic acid signaling homeostasis is essential for seed storability. The molecular chaperone OsCPN10a enhances rice seed storability by forming a trimeric chaperone complex with OsCPN20‐OsHSP60‐3B that attenuates abscisic acid signaling via direct interaction with OsPYL10‐OsABIL1, thereby maintaining starch integrity and offering a promising target ...
Sufeng Liao, Yidong Wei, Yongsheng Zhu, Kunyang Li, Ting Chen, Shuai Zhao, Fangxi Wu, Jinlan Wang, Pengbo Yu, Hongguang Xie, Liping Chen, Qiuhua Cai, Huaan Xie, Jianfu Zhang   +13 more
wiley   +1 more source