Results 51 to 60 of about 394,889 (261)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Mucosal Immunization Has Benefits over Traditional Subcutaneous Immunization with Group A Streptococcus Antigens in a Pilot Study in a Mouse Model

Vaccines, 2023
Group A Streptococcus (GAS) is a major human pathogen for which there is no licensed vaccine. To protect against infection, a strong systemic and mucosal immune response is likely to be necessary to prevent initial colonization and any events that might ...
Helen Alexandra Shaw, Alex Remmington, Giselle McKenzie, Caroline Winkel, Fatme Mawas   +4 more
doaj   +1 more source

Population gene introgression and high genome plasticity for the zoonotic pathogen Streptococcus agalactiae [PDF]

, 2019
The influence that bacterial adaptation (or niche partitioning) within species has on gene spillover and transmission among bacteria populations occupying different niches is not well understood.
Abbott, Abby, Almeida, Baily, Bankevich, Beerli, Beerli, Benjamini, Bertels, Bikard, Bisharat, Bishop, Bohnsack, Borchardt, Brett M Probert, Brochet, Bruen, Brynildsrud, Capella-Gutierrez, Chen, Chen, Cheng, Chiara Crestani, Chopra, Christopher D Town, Conrad, Croucher, Da Cunha, Delannoy, Delannoy, Dogan, Edgar, Enright, Erwin, Fernandez, Ferreira, Flores, Fluegge, Garrett H Springer, Gauthier, Glazko, Glazko, Greig, Guglielmini, Gupta, Hayley B Hassler, Heaps, Holt, Imperi, Inouye, Irina M Velsko, Jafar, Jaskowiak, Jeukens, Johri, Jones, Jones, Jorgensen, Joubrel, Kalimuddin, Kim, Konig, Langdon, Librado, Lin, Lindahl, Liu, Liu, Lopez-Sanchez, Loytynoja, Lyhs, Manning, Manning, Martins, Marttinen, Mather, McArthur, Md Tauqeer Alam, Michael J Stanhope, Morse, Murrell, Page, Pal, Paulina D Pavinski Bitar, Pedersen, Petrovska, Pond, Poyart, Price, Qin, Richards, Richards, Richards, Rosinski-Chupin, Ruth N Zadoks, Sahl, Sahl, Sahl, Scheffer, Schrieber, Seemann, Shannon D Manning, Shapiro, Shepheard, Sheppard, Spoor, Springman, Srivastava, Stamatakis, Stoddard, Sukhnanand, Supek, Tettelin, Tettelin, Tian, van der Mee-Marquet, Verani, Viana, Vincent P Richards, Yu, Zadoks, Zankari, Zerbino, Zhang, Zhu   +124 more
core   +2 more sources

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source

Treatment of acute pharyngitis in children: an Italian intersociety consensus (SIPPS-SIP-SITIP-FIMP-SIAIP-SIMRI-FIMMG)

Italian Journal of Pediatrics
Sore throat represents one of the main causes of antibiotic overprescription in children. Its management is still a matter of debate, with countries considering streptococcal pharyngotonsillitis a benign and self-limiting condition and others advocating ...
Elena Chiappini, Giovanni Simeone, Marcello Bergamini, Roberta Pellegrino, Alfredo Guarino, Annamaria Staiano, Susanna Esposito, Guido Castelli Gattinara, Andrea Lo Vecchio, Stefania Stefani, Iride Dello Iacono, Immacolata Scotese, Giovanna Tezza, Giulio Dinardo, Simona Riccio, Sofia Pellizzari, Sonia Iavarone, Giulia Lorenzetti, Elisabetta Venturini, Daniele Donà, Luca Pierantoni, Mattia Doria, Silvia Garazzino, Fabio Midulla, Claudio Cricelli, Luigi Terracciano, Annalisa Capuano, Eugenia Bruzzese, Daniele Ghiglioni, Lara Fusani, Eleonora Fusco, Paolo Biasci, Lamberto Reggiani, Luigi Matera, Enrica Mancino, Elisa Barbieri, Antonio D’Avino, Laura Cursi, Maria Giuseppa Sullo, Silvestro Scotti, Gian Luigi Marseglia, Giuseppe Di Mauro, Nicola Principi, Luisa Galli, Maria Carmen Verga   +44 more
doaj   +1 more source

Impact of bidirectional relationships between streptococcus anginosus group and host tissue matrix components on cellular activity: Role in establishment of infection [PDF]

, 2003
This paper investigates pathogenic mechanisms of the Streptococcus anginosus group (SAG) of bacteria which influence the biological activity of periodontal ligament (PDL) cells, endothelial cells and also how matrix proteins produced by these host cells ...
Waddington, Rachel J., Landrygan-Bakri, Janine, Wilson, Melanie, Williams, David W., Lewis, Michael Alexander Oxenham   +4 more
core   +10 more sources

Cutaneous Melanoma Drives Metabolic Changes in the Aged Bone Marrow Immune Microenvironment

Aging and Cancer, EarlyView.
Melanoma, the deadliest form of skin cancer, increasingly affects older adults. Our study reveals that melanoma induces changes in iron and lipid levels in the bone marrow, impacting immune cell populations and increasing susceptibility to ferroptosis.
Alexis E. Carey, Murilo Ramos Rocha, Cheyenne Palm, Vania Wang, Denys Balandin, Ethan Black, Kevin Y. Zhang, Fan Huang, Daniel J. Zabransky, Yash Chhabra, Mareike Peters, Gabriel Ghiaur, Ashani Weeraratna   +12 more
wiley   +1 more source

Prevalent emm Types among Invasive GAS in Europe and North America since Year 2000

Frontiers in Public Health, 2018
BackgroundStreptococcus pyogenes or group A streptococcus (GAS) is an important human pathogen responsible for a broad range of infections, from uncomplicated to more severe and invasive diseases with high mortality and morbidity.
Giovanni Gherardi, Luca Agostino Vitali, Roberta Creti   +2 more
doaj   +1 more source

Playing With Fire: Proinflammatory Virulence Mechanisms of Group A Streptococcus

Frontiers in Cellular and Infection Microbiology, 2021
Group A Streptococcus is an obligate human pathogen that is a major cause of infectious morbidity and mortality. It has a natural tropism for the oropharynx and skin, where it causes infections with excessive inflammation due to its expression of ...
Shyra Wilde, Anders F. Johnson, Christopher N. LaRock, Christopher N. LaRock   +3 more
doaj   +1 more source

ApuA, a multifunctional x-glucan-degrading enzyme of Streptococcus suis, mediates adhesion to porcine epithelium and mucus [PDF]

, 2010
We have identified apuA in Streptococcus suis, which encodes a bifunctional amylopullulanase with conserved -amylase and pullulanase substrate-binding domains and catalytic motifs.
Ferrando, M.L., Fuentes, S., Greeff, A., de, Smith, H.E., Wells, J.   +4 more
core   +2 more sources