Results 1 to 10 of about 180,891 (150)

GTP: Gatekeeper for Autophagy [PDF]

open access: yesMolecular Cell, 2010
Chaperone-mediated autophagy (CMA) is involved in cellular quality control, and in the response to stress, deterioration in CMA contributes to the aging phenotype and to various disorders. The paper by Bandyopadhyay et al. in this issue of Molecular Cell demonstrates the role of GTP binding by elongation factor 1 alpha (EF1alpha) in regulating the ...
Isabelle Beau, Patrice Codogno
openaire   +3 more sources

GTP-Yeast Actin [PDF]

open access: yesJournal of Biological Chemistry, 2002
Because of the apparently greater conformational flexibility of yeast versus muscle actin and the ability of other members in the actin protein superfamily to efficiently use both ATP and GTP, we assessed the ability of yeast actin to function with GTP.
Xiaoyi Yao   +2 more
openaire   +3 more sources

Kinetic analysis of GTP hydrolysis catalysed by the Arf1-GTP-ASAP1 complex [PDF]

open access: yesBiochemical Journal, 2007
Arf (ADP-ribosylation factor) GAPs (GTPase-activating proteins) are enzymes that catalyse the hydrolysis of GTP bound to the small GTP-binding protein Arf. They have also been proposed to function as Arf effectors and oncogenes. We have set out to characterize the kinetics of the GAP-induced GTP hydrolysis using a truncated form of ASAP1 [Arf GAP with ...
Ruibai Luo   +6 more
openaire   +4 more sources

RalA and RalB Function as the Critical GTP Sensors for GTP-Dependent Exocytosis [PDF]

open access: yesThe Journal of Neuroscience, 2007
Although it has been established that the activation of GTPases by non-hydrolyzable GTP stimulates neurotransmitter release from many different secretory cell types, the underlying mechanisms remain unclear. In the present study we aimed to elucidate the functional role(s) for endogenous Ras-like protein A (RalA) and RalB GTPases in GTP-dependent ...
Lakshmanan Arunachalam   +10 more
openaire   +3 more sources

GTP cyclohydrolase I utilizes metal‐free GTP as its substrate [PDF]

open access: yesEuropean Journal of Biochemistry, 2003
GTP cyclohydrolase I (GCH) is the rate‐limiting enzyme for the synthesis of tetrahydrobiopterin and its activity is important in the regulation of monoamine neurotransmitters such as dopamine, norepinephrine and serotonin. We have studied the action of divalent cations on the enzyme activity of purified recombinant human GCH expressed in Escherichia ...
T. Suzuki, H. Kurita, H. Ichinose
openaire   +3 more sources

The speed of GTP hydrolysis determines GTP cap size and controls microtubule stability [PDF]

open access: yeseLife, 2019
ABSTRACTMicrotubules are bistable cytoskeletal polymers whose function depends on their property to switch between states of growth and shrinkage1. Growing microtubules are thought to be stabilized by a GTP cap at their ends2-5. The nature of this cap, however, is still poorly understood.
Johanna Roostalu   +5 more
openaire   +5 more sources

Comprehensive Characterization of SGTP-Binding Proteins by Orthogonal Quantitative SGTP-Affinity Profiling and SGTP/GTP Competition Assays

open access: yesAnalytical Chemistry, 2014
Thiopurine drugs are widely used as antileukemic drugs and immunosuppressive agents, and 6-thioguanosine triphosphate ((S)GTP) is a major metabolite for these drugs. Recent studies have suggested that thiopurine drugs may exert their cytotoxic effects partly through binding of (S)GTP to a GTP-binding protein, Rac1.
Debin Ji   +3 more
openaire   +3 more sources

Interaction between chromium GTP and tubulin. Stereochemistry of GTP binding, GTP hydrolysis, and microtubule stabilization

open access: yesJournal of Biological Chemistry, 1991
Chromium GTP (CrGTP) has been used to probe the stereochemistry of metal-GTP binding to exchangeable site of tubulin and to examine the fate and role of nucleotide-bound metal ion in GTP hydrolysis associated with microtubule assembly. The absolute stereoconfiguration of the two pairs of diastereomers of beta,gamma-bidentate CrGTP has been determined ...
C Valentin-Ranc   +2 more
openaire   +3 more sources

An intermolecular G-quadruplex as the basis for GTP recognition in the class V–GTP aptamer [PDF]

open access: yesRNA, 2016
Many naturally occurring or artificially created RNAs are capable of binding to guanine or guanine derivatives with high affinity and selectivity. They bind their ligands using very different recognition modes involving a diverse set of hydrogen bonding and stacking interactions.
Nasiri, Amir Hossein   +4 more
openaire   +5 more sources

Crystallographic Evidence for Substrate-Assisted GTP Hydrolysis by a Small GTP Binding Protein [PDF]

open access: yesStructure, 2005
GTP hydrolysis by small GTP binding proteins of the Ras superfamily is a universal reaction that controls multiple cellular regulations. Its enzymic mechanism has been the subject of long-standing debates as to the existence/identity of the general base and the electronic nature of its transition state.
Jacqueline Cherfils   +1 more
openaire   +3 more sources

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