Results 351 to 360 of about 374,227 (387)
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Enzyme, 1979
The cytosolic form of phosphoenolpyruvate carboxykinase (GTP; EC 4.1.1.32) from rat liver was purified by a procedure involving affinity chromatography on agarose-hydrazide-GTP. Phosphoenolpyruvate carboxykinase is retained quantitatively by the affinity medium in the presence of manganese and can be specifically eluted by a pulse of GTP.
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The cytosolic form of phosphoenolpyruvate carboxykinase (GTP; EC 4.1.1.32) from rat liver was purified by a procedure involving affinity chromatography on agarose-hydrazide-GTP. Phosphoenolpyruvate carboxykinase is retained quantitatively by the affinity medium in the presence of manganese and can be specifically eluted by a pulse of GTP.
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Stabilization of microtubules by GTP analogues
Biochemical and Biophysical Research Communications, 1990We recently demonstrated that the nonhydrolyzable analogues of GTP (GMPPCP and GMPPNP) and ATP support the elongation phase of tubulin assembly and are incorporated into the E-site of polymerized tubulin. In this report we studied the stability of microtubules containing GTP analogues by examining length redistributions after shearing at polymer steady
Magdalena R. Mejillano +2 more
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The effect of GTP on benzodiazepine receptors
European Journal of Pharmacology, 1982GTP decreases the specific binding of [3H]flunitrazepam to cerebral cortical membrane sites. Scatchard analysis data show that GTP does not alter the maximum binding sites (Bmax), but increases the dissociation constant (KD) for [3H]flunitrazepam. GTP also inhibits the specific binding of the benzodiazepine antagonist [3H]CGS-8216. The specific binding
Jim C. Fong +2 more
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Annual Review of Cell and Developmental Biology, 2013
Shape changes and topological remodeling of membranes are essential for the identity of organelles and membrane trafficking. Although all cellular membranes have common features, membranes of different organelles create unique environments that support specialized biological functions.
Tina H. Lee +4 more
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Shape changes and topological remodeling of membranes are essential for the identity of organelles and membrane trafficking. Although all cellular membranes have common features, membranes of different organelles create unique environments that support specialized biological functions.
Tina H. Lee +4 more
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Current Opinion in Structural Biology, 1991
Abstract There has been much progress this year in characterizing the complex series of post-translational modifications important for GTP-binding protein function. In addition, the long-awaited molecular characterization of a G protein that regulates phospholipase C activity has been reported.
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Abstract There has been much progress this year in characterizing the complex series of post-translational modifications important for GTP-binding protein function. In addition, the long-awaited molecular characterization of a G protein that regulates phospholipase C activity has been reported.
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Expression cloning to identify monomeric GTP-binding proteins by GTP overlay
2001Publisher Summary More than 70 small Ras-related GTP-binding proteins have been identified and additional members of the family continue to be discovered. The majority of these proteins have been isolated by screening genomic or cDNA libraries at low stringency with oligonucleotide mixes corresponding to the conserved guanine nucleotide-binding ...
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Determination of GTP loading on Rho
2000Publisher Summary This chapter discusses the determination of guanosine 5'-triphosphate (GTP) loading on Rho. Rho is a member of the Ras superfamily of low molecular weight GTPase that is implicated in the regulation of actin cytoskeleton organization.
Martin A. Schwartz, Xiang-Dong Ren
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Hydrolysis of GTP by the α‐chain of Gs and other GTP binding proteins
Proteins: Structure, Function, and Bioinformatics, 1989AbstractThe functions of G proteins—like those of bacterial elongation factor (EF) Tu and the 21 kDa ras proteins (p21ras)—depend upon their abilities to bind and hydrolyze GTP and to assume different conformations in GTP‐ and GDP‐bound states. Similarities in function and amino acid sequence indicate that EF‐Tu, p21ras, and G protein α‐chains evolved ...
Henry R. Bourne +2 more
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GTP binding and growth control
Current Opinion in Cell Biology, 1990Guanosine triphosphate (GTP)-binding proteins are involved, directly or indirectly, in virtually every aspect of cellular growth control and metabolism. At least three major classes of GTP-binding protein have been identified. The first includes elongation factors and initiation factors that are part of the machinery of protein synthesis, and also part
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An Expression Cloning Method to Identify Monomeric GTP-Binding Proteins by GTP Overlay
Analytical Biochemistry, 1997We have developed a method for identifying monomeric GTP-binding proteins that is based on probing plasmid expression libraries with [alpha-32P]GTP. The method involves the production of nitrocellulose replica filter lifts from a plasmid cDNA expression library and treatment of the filters with chloroform vapor to lyse the Escherichia coli and to ...
Keiko Kadono-Okuda, Douglas A. Andres
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