Results 111 to 120 of about 2,866 (140)

Differential effects of guanosine 5′-O-(3-thiotriphosphate) (GTPγS) on the 2-[125I]iodomelatonin binding sites in the chicken bursa of Fabricius and spleen

Neuroscience Letters, 1994
Effects of 10 and 50 mumol/l guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) on 2-[125I]iodomelatonin binding in the chicken bursa of Fabricius and spleen were tested. In the chicken bursa of Fabricius, GTP gamma S increased the Kd of 2-[125I]iodomelatonin binding sites without affecting the Bmax value.
Poon, AMS, Pang, SF
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Catecholamine-stimulated guanosine 5'-O-(3-thiotriphosphate) binding to the stimulatory GTP-binding protein of adenylate cyclase: kinetic analysis in reconstituted phospholipid vesicles

Biochemistry, 1984
The stimulatory GTP-binding protein of adenylate cyclase, Gs, and beta-adrenergic receptors were reconstituted into unilamellar phospholipid vesicles. The kinetics of the quasiirreversible binding of guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) to Gs, equivalent to Gs activation by nucleotide, was studied with respect to the stimulation of this ...
T, Asano, E M, Ross
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Plasma membrane associated phospholipase C from human platelets: synergistic stimulation of phosphatidylinositol 4,5-bisphosphate hydrolysis by thrombin and guanosine 5'-O-(3-thiotriphosphate)

Biochemistry, 1989
The effects of thrombin and GTP gamma S on the hydrolysis of phosphoinositides by membrane-associated phospholipase C (PLC) from human platelets were examined with endogenous [3H]inositol-labeled membranes or with lipid vesicles containing either [3H]phosphatidylinositol or [3H]phosphatidylinositol 4,5-bisphosphate.
J J, Baldassare, P A, Henderson, G J, Fisher   +2 more
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Guanosine 5′-O-(3-Thiotriphosphate)-induced O−2Generation in Permeabilized Neutrophils Requires Protein Kinase C and Phospholipase C but Not Tyrosine Kinase or Phospholipase D

Archives of Biochemistry and Biophysics, 1999
Guanosine 5'-O-(3-thiotriphosphate) (GTPgammaS) induces respiratory burst (O-2 generation) in permeabilized human neutrophils. The signal pathway from GTPgammaS to the enzyme responsible for O-2 generation (NADPH oxidase) is not well defined. To elucidate the signaling pathway activated by GTPgammaS, we used selective inhibitors to test for the ...
M, Tamura, K, Yoshida, K, Kataoka
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Cell-free translocation of recombinant p47-phox, a component of the neutrophil NADPH oxidase: effects of guanosine 5'-O-(3-thiotriphosphate), diacylglycerol, and an anionic amphiphile

Biochemistry, 1992
We reported previously that diacylglycerol (diC8) and GTP gamma S synergize with an anionic amphiphile such as sodium dodecyl sulfate (SDS) to produce high rates of superoxide generation in a cell-free system consisting of neutrophil plasma membrane plus cytosol [Burnham, D. N., Uhlinger, D. J., & Lambeth, J. D. (1990) J. Biol. Chem. 265, 17550-17559].
S R, Tyagi, N, Neckelmann, D J, Uhlinger, D N, Burnham, J D, Lambeth   +4 more
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Effect of Guanosine 5′‐O‐(3‐Thiotriphosphate) and Calcium on γ‐Aminobutyric Acid_B Binding as a Function of Postnatal Development

Journal of Neurochemistry, 1989
Abstract: We have examined the development of γ‐aminobutyric acidB (GABAB) receptors in rat cerebrum using a binding assay that has achieved specific binding levels of ∼50% with the GABAB ligand (–)‐[3H]baclofen. As early as postnatal day 1, GABAB receptors are present and are linked to both calcium‐ and guanosine triphosphate‐binding protein (G ...
M I, al-Dahan, R H, Thalmann
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Combination of Arachidonic Acid and Guanosine 5′-O-(3-Thiotriphosphate) Induces Translocation of rac p21s to Membrane and Activation of NADPH Oxidase in a Cell-Free System

Biochemical and Biophysical Research Communications, 1993
The superoxide-generating NADPH oxidase system in phagocytes consists of membrane-associated cytochrome b558 and three cytosolic components named p67-phox, p47-phox, and rac p21s. In a cell-free system consisting of membrane and cytosol, the oxidase can be activated with guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) and an unsaturated fatty acid ...
T, Sawai, M, Asada, H, Nunoi, I, Matsuda, S, Ando, T, Sasaki, K, Kaibuchi, Y, Takai, K, Katayama   +8 more
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Cholesterol-Dependent Association of Caveolin-1 with the Transducin α Subunit in Bovine Photoreceptor Rod Outer Segments:  Disruption by Cyclodextrin and Guanosine 5‘-O-(3-Thiotriphosphate)

Biochemistry, 2003
Evidence suggests that caveolins, 21-24 kDa cholesterol-binding proteins that generally reside in specialized detergent-resistant membrane microdomains, act as signaling scaffolds. Detergent-resistant membranes isolated from rod outer segments (ROS) have been previously shown to contain the photoreceptor G-protein, transducin.
Michael H, Elliott, Steven J, Fliesler, Abboud J, Ghalayini   +2 more
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2-[125I]iodomelatonin binding sites in the quail liver: characterization and the effect of guanosine 5'-O-(3-thiotriphosphate).

Biological signals, 1995
Melatonin receptors were studied in quail livers using the melatonin agonist 2-[125I]iodomelatonin ([125I]MEL) as the radioligand. The specific binding of [125I]MEL to membrane preparations of liver was rapid, stable, saturable, reversible and of high affinity.
Q, Wan, S F, Pang
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Rapid binding of guanosine 5'-O-(3-thiotriphosphate) to an apparent complex of beta-adrenergic receptor and the GTP-binding regulatory protein Gs.

Biochemistry, 1988
When reconstituted phospholipid vesicles that contain purified beta-adrenergic receptors and the GTP-binding regulatory protein Gs were preincubated with agonist before the addition of guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S), the typical receptor-stimulated GTP gamma S binding reaction was preceded by an even more rapid burst of GTP gamma S ...
D C, May, E M, Ross
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