Interaction of the Xanthine Nucleotide Binding Goα Mutant with G Protein-coupled Receptors [PDF]
, 1998 We constructed a double mutant version of the α subunit of Go that was regulated by xanthine nucleotides instead of guanine nucleotides (GoαX). We investigated the interaction between GoαX and G protein-coupled receptors in vitro.
Simon, Melvin I., Yu, Bo
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Ca2+-Stimulated Catecholamine Release from alpha-Toxin Permeabilized PC12 Cells [PDF]
, 1987 Two possible cellular pathways of catecholamines from the chromaffin vesicles of PC 12 cells to the surrounding medium are explored in this study. The direct one circumventing the cytoplasm can be activated in a-toxin-permeabilized cells with micromolar ...
Ahnert-Hilger, G. +2 more
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Interlinked GTPase cascades provide a motif for both robust switches and oscillators [PDF]
J. R. Soc. Interface 16, 20190198 (2019), 2019 GTPases regulate a wide range of cellular processes, such as intracellular vesicular transport, signal transduction, and protein translation. These hydrolase enzymes operate as biochemical switches by toggling between an active guanosine triphosphate (GTP)-bound state and an inactive guanosine diphosphate (GDP)-bound state.
arxiv +1 more source
Kinetics and activation of phospholipase C by P2Y purinergic agonists and guanine nucleotides [PDF]
, 1989 Membranes prepared from [3H]inositol-labeled turkey erythrocytes express a phospholipase C that is markedly stimulated by stable analogs of GTP (Harden, T. K., Stephens, L., Hawkins, P. T., and Downes, C. P. (1987) J. Biol. Chem. 262, 9057-9061).
Boyer, JL, Downes, CP, Harden, T
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UV resistance of nucleosides -- an experimental approach [PDF]
, 2020 The emergence of life on Earth is a highly discussed but still unsolved question. Current research underlines the importance of environments within close proximity to the Earth's surface as they can solve long-standing problems such as polymerization of nucleotides and phosphorylation of nucleosides.
arxiv +1 more source
Functional Identification of Catalytic Metal Ion Binding Sites within RNA [PDF]
, 2023 The viability of living systems depends inextricably on enzymes that catalyze phosphoryl transfer reactions. For many enzymes in this class, including several ribozymes, divalent metal ions serve as obligate cofactors.
Herschlag, Daniel +3 more
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Impaired desensitization of a human polymorphic α2B-adrenergic receptor variant enhances its sympatho-inhibitory activity in chromaffin cells [PDF]
, 2011 Background α2-adrenergic receptors (ARs) mediate many cellular actions of epinephrine and norepinephrine and inhibit their secretion from adrenal chromaffin cells.
Kassimatis, Theodoros +2 more
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An Information Theoretical Analysis of Kinase Activated Phosphorylation Dephosphorylation Cycle [PDF]
IEEE Transactions on NanoBioscience, Volume 11, Issue 3, pp. 289 -
295 (2012), 2011 Signal transduction, the information processing mechanism in biological cells, is carried out by a network of biochemical reactions. The dynamics of driven biochemical reactions can be studied in terms of nonequilibrium statistical physics. Such systems may also be studied in terms of Shannon's information theory.
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Reduction of Muscarinic Receptor Density and of Guanine Nucleotide-Stimulated Phosphoinositide Hydrolysis in Human SH-SY5Y Neuroblastoma Cells Following Long-Term Treatment with 12- O -Tetradecanoylphorbol 13-Acetate or Mezerein [PDF]
, 1990 The actions of tumor promoters on the coupling of muscarinic receptors to the hydrolysis of inositol lipids and the generation of Ca 2+ signals were examined in the human neuroblastoma SH-SY5Y cell line.
Cioffi, Catherine L., Fisher, Stephen K.
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A thermodynamic paradigm for solution demixing inspired by nuclear transport in living cells [PDF]
Phys. Rev. Lett. 118, 158101 (2017), 2016 Living cells display a remarkable capacity to compartmentalize their functional biochemistry. A particularly fascinating example is the cell nucleus. Exchange of macromolecules between the nucleus and the surrounding cytoplasm does not involve traversing a lipid bilayer membrane.
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