Uncoupling conformational change from GTP hydrolysis in a heterotrimeric G protein alpha-subunit [PDF]
, 2004 Heterotrimeric G protein alpha (G alpha) subunits possess intrinsic GTPase activity that leads to functional deactivation with a rate constant of approximately 2 min(-1) at 30 degrees C.
Du, Xinlin +5 more
core +1 more source
Barium and Calcium Stimulate Secretion from Digitonin-Permeabilized Bovine Adrenal Chromaffin Cells by Similar Pathways [PDF]
, 1992 We compared the characteristics of secretion stimulated by EGTA-buffered Ba 2+ - and Ca 2+ -containing solutions in digitonin-permeabilized bovine adrenal chromaffin cells.
Daniel R. Terbush +3 more
core +1 more source
Transport in hybrid electronic devices based on a modified DNA nucleoside (deoxyguanosine) [PDF]
arXiv, 2000 We report on a new class of hybrid electronic devices based on a DNA nucleoside (deoxyguanosine lipophilic derivative) whose assembled polymeric ribbons interconnect a submicron metallic gate. The device exhibits large conductivity at room temperature, rectifying behaviour and strong current-voltage hysteresis.
arxiv
Pilot study of electrochemical reduction of selected nucleotides and double-stranded DNA at pristine micro-/ultrananocrystalline boron-doped diamond electrodes at very negative potentials [PDF]
Pristine polycrystalline boron-doped diamond electrodes (BDDEs) -- microcrystalline (B-MCDE) and ultrananocrystalline (B-UNCDE) were applied for the electrochemical reduction of several selected purine nucleotides -- Guanosine 5'-monophosphate (GMP), 2'-Deoxyguanosine 5'-monophosphate (dGMP), Adenosine 5'-monophosphate (AMP), Adenosine 5'-diphosphate ...
arxiv +1 more source
How the sperm triggers the fertilisation calcium wave in the sea urchin egg [PDF]
, 1990 Fertilisation is characterised by an increase in cytosolic free calcium (Ca). This crucial event transforms the egg, releasing it from its state of cell cycle arrest and stimulating embryonic development.
Crossley, Ian Brian
core
Screening the Thermotoga maritima genome for new wide-spectrum nucleoside and nucleotide kinases. [PDF]
J Biol Chem, 2023 Winkler KF +7 more
europepmc +1 more source
Versatile strategy using vaccinia virus-capping enzyme to synthesize functional 5' cap-modified mRNAs. [PDF]
Nucleic Acids Res, 2023 Ohno H +6 more
europepmc +1 more source
Loss of Cannabinoid‐Stimulated Guanosine 5′‐O‐(3‐[35S]Thiotriphosphate) Binding Without Receptor Down‐Regulation in Brain Regions of Anandamide‐Tolerant Rats [PDF]
, 2000 Tiziana Rubino +4 more
openalex +1 more source
Transition state structures and the roles of catalytic residues in GAP-facilitated GTPase of Ras as elucidated by (18)O kinetic isotope effects [PDF]
, 2009 Ras-catalyzed guanosine 5\u27 triphosphate (GTP) hydrolysis proceeds through a loose transition state as suggested in our previous study of (18)O kinetic isotope effects (KIE) [ Du , X. et al. ( 2004 ) Proc. Natl. Acad. Sci. U.S.A.
Du, Xinlin, Sprang, Stephen R
core +1 more source
Synthesis and biochemical evaluation of 17-N-beta-aminoalkyl-4,5α-epoxynormorphinans. [PDF]
Sci Rep, 2023 Ötvös F +8 more
europepmc +1 more source