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Guanosine tetra- and pentaphosphate analysis
Microchemical Journal, 1975Abstract A system is presented that can separate and quantitate in picomole amounts various guanosine tetra and penta phosphates namely guanosine 5′ triphosphate, 3′ diphosphate (pppGpp), guanosine 5′ tetraphosphate (ppppG), and diguanosine 5′ tetraphosphate (GppppG).
Knox Van Dyke
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Archives of Microbiology, 1981
The intracellular levels of ppGpp and pppApp in various microorganisms were determined by radioimmunoassay, with was of more accuracy and convenience than previous 32P-labeling method. ppGpp was detected in bacteria, actinomycetes, yeasts and fungi. pppApp was found in non-spore forming bacteria such as the genera Pseudomonas and Escherichia, fungi and
Yasutaro Hamagishi, Oki Toshikazu
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The intracellular levels of ppGpp and pppApp in various microorganisms were determined by radioimmunoassay, with was of more accuracy and convenience than previous 32P-labeling method. ppGpp was detected in bacteria, actinomycetes, yeasts and fungi. pppApp was found in non-spore forming bacteria such as the genera Pseudomonas and Escherichia, fungi and
Yasutaro Hamagishi, Oki Toshikazu
exaly +2 more sources
Inorganic polyphosphate (poly-P) molecules are linear polymers of orthophosphate units linked by ‘high-energy’ phosphoanhydride bonds. In bacteria, poly-P is involved in many physiological processes such as cellular metabolism, growth, motility, ‘stress-responses’, virulence, quorum sensing and biofilm formation.
Watt, RM, Tang, NY, Lu, B
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Guanosine tetra- and pentaphosphate synthesis by bacterial stringent factor and eukaryotic ribosomes
Nature, 1977AMINO acid starvation of rel+ bacteria1 results in a rapid pleiotropic response involving the cessation of stable RNA synthesis, a decrease in the rate of nucleoside transport, an altered pattern of mRNA synthesis, inhibition of phospholipid synthesis and increased protein turnover.
J W, Pollard, J, Parker
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Analytical Biochemistry, 1988
Commercial samples of GTP and guanosine 5'-tetraphosphate were analyzed, with or without previous treatment with alkaline phosphatase, by high-pressure liquid chromatography on a Hypersil ODS column. They showed the presence of diguanosine 5',5"'-Pl,Pn-tri, tetra-, and pentaphosphates in varying amounts depending on the sample, but usually in ...
F, Buitrago, J, Canales, A, Sillero
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Commercial samples of GTP and guanosine 5'-tetraphosphate were analyzed, with or without previous treatment with alkaline phosphatase, by high-pressure liquid chromatography on a Hypersil ODS column. They showed the presence of diguanosine 5',5"'-Pl,Pn-tri, tetra-, and pentaphosphates in varying amounts depending on the sample, but usually in ...
F, Buitrago, J, Canales, A, Sillero
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Biochemical and Biophysical Research Communications, 1974
Abstract Salt-washed ribosomes from Escherichia coli , plus stringent protein, form more ppGpp than pppGpp from GTP at all times, but unwashed ribosomes are shown to synthesize primarily pppGpp as the initial product.
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Abstract Salt-washed ribosomes from Escherichia coli , plus stringent protein, form more ppGpp than pppGpp from GTP at all times, but unwashed ribosomes are shown to synthesize primarily pppGpp as the initial product.
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European Journal of Biochemistry, 1976
tRNAphe from yeast modified at the 3′‐terminal adenosine residue or missing part of its CpCpA end was investigated for its ability to participate in the synthesis of guanosine tetraphosphate and pentaphosphate using Escherichia coli ribosomes. Only tRNA containing complete CpCpA end and an intact ribose residue in the terminal adenosine was active in ...
M, Sprinzl, D, Richter
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tRNAphe from yeast modified at the 3′‐terminal adenosine residue or missing part of its CpCpA end was investigated for its ability to participate in the synthesis of guanosine tetraphosphate and pentaphosphate using Escherichia coli ribosomes. Only tRNA containing complete CpCpA end and an intact ribose residue in the terminal adenosine was active in ...
M, Sprinzl, D, Richter
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