Results 1 to 10 of about 46,501 (205)

An Interplay among FIS, H-NS, and Guanosine Tetraphosphate Modulates Transcription of the Escherichia coli cspA Gene under Physiological Growth Conditions [PDF]

Frontiers in Molecular Biosciences, 2016
CspA, the most characterized member of the csp gene family of Escherichia coli, is highly expressed not only in response to cold stress, but also during the early phase of growth at 37°C.
Anna Brandi, Mara Giangrossi, Maurizio Falconi   +2 more
exaly   +7 more sources

A guanosine tetraphosphate (ppGpp) mediated brake on photosynthesis is required for acclimation to nitrogen limitation in Arabidopsis [PDF]

eLife, 2022
Guanosine pentaphosphate and tetraphosphate (together referred to as ppGpp) are hyperphosphorylated nucleotides found in bacteria and the chloroplasts of plants and algae.
Shanna Romand, Hela Abdelkefi, Cécile Lecampion, Mohamed Belaroussi, Melanie Dussenne, Brigitte Ksas, Sylvie Citerne, Jose Caius, Stefano D'Alessandro, Hatem Fakhfakh, Stefano Caffarri, Michel Havaux, Ben Field   +12 more
doaj   +3 more sources

Guanosine Tetraphosphate Has a Similar Affinity for Each of Its Two Binding Sites on Escherichia coli RNA Polymerase [PDF]

Frontiers in Microbiology, 2020
During nutrient deprivation, the bacterial cell undergoes a stress response known as the stringent response. This response is characterized by induction of the nucleotide derivative guanosine tetraphosphate (ppGpp) that dramatically modulates the cell’s ...
Angela R. Myers, Danielle P. Thistle, Wilma Ross, Richard L. Gourse   +3 more
doaj   +3 more sources

Persistence Increases in the Absence of the Alarmone Guanosine Tetraphosphate by Reducing Cell Growth. [PDF]

Sci Rep, 2016
AbstractMost bacterial cells are stressed and as a result, some become tolerant to antibiotics by entering a dormant state known as persistence. The key intracellular metabolite that has been linked to this persister state is guanosine tetraphosphate (ppGpp), the alarmone that was first linked to nutrient stress.
Chowdhury N, Kwan BW, Wood TK.
europepmc   +5 more sources

Improved plant biomass production under low nitrogen conditions through conditional accumulation of the second messenger, guanosine tetraphosphate, in chloroplasts and mitochondria [PDF]

Frontiers in Plant Science
To enhance plant biomass production under low nitrogen conditions, we employed a method to artificially and temporarily accumulate the bacterial second messenger, guanosine tetraphosphate (ppGpp), to modify plastidial or mitochondrial metabolism ...
Mina Goto, Takanari Nemoto, Kazuma Sakoda, Atsushi Sakurai, Sousuke Imamura, Shinji Masuda   +5 more
doaj   +3 more sources

Accumulation of guanosine tetraphosphate and guanosine pentaphosphate in Myxococcus xanthus during starvation and myxospore formation [PDF]

Journal of Bacteriology, 1980
Cultures of Myxococcus xanthus develop multicellular fruiting bodies when starved for carbon and nitrogen sources on an agar surface. Under these conditions of severe starvation, cultures rapidly accumulated a compound identified as guanosine tetraphosphate by chromatographic migration of the compound and of its major acid and alkali breakdown products.
C, Manoil, D, Kaiser
exaly   +4 more sources

Conditions that trigger guanosine tetraphosphate accumulation in Caulobacter crescentus [PDF]

Journal of Bacteriology, 1981
Caulobacter crescentus accumulated guanosine tetraphosphate in response to nitrogen starvation but not in response to amino acid starvation. Nitrogen starvation also acted specifically to inhibit certain transitions in the C. crescentus life cycle, and guanosine tetraphosphate may act as an intracellular regulator of cell cycle events.
J Gallant, N Agabian
exaly   +4 more sources

Controlled Delivery and Light-Induced Release of Magic Spot Nucleotides in Escherichia coli. [PDF]

Angew Chem Int Ed Engl
We synthesized photocaged, clickable and isotope‐labeled “magic spot” Nucleotides and delivered them into Escherichia coli comparing different approaches. Light‐controlled release inside living cells enabled tracking of their conversion from pppGpp to ppGpp by capillary electrophoresis mass spectrometry and revealed their impact on the growth rate of a
Popp C, Moser P, Schwoerbel A, Liu X, Freitag J, Sarmah P, Prucker I, Zscherp R, Wang X, Klahn P, Koch HG, Bange G, Jessen HJ.   +12 more
europepmc   +2 more sources

The 5' Cap Epitranscriptome and Beyond: Natural and Engineered 5' Cap Modifications for Optimizing mRNA Therapeutics and Functional Studies. [PDF]

ChemMedChem
5′ Cap modifications can be introduced via enzymes or chemical synthesis. Natural and non‐natural 5′ cap structures affect mRNA properties, including translation (T), stability (S), and/or immunogenicity (I). Eukaryotic mRNAs made by in vitro transcription have emerged as medical modalities for vaccination and protein replacement therapy. The 5′ cap is
Dahm GC, Pieper M, Schepers H, Rentmeister A.   +3 more
europepmc   +2 more sources

Bacterial alarmone (p)ppGpp mediates the pathogenicity of Clavibacter michiganensis via a dual mechanism that affects both enzyme production and the Tat secretion system [PDF]

mSystems
The gram-positive bacterium Clavibacter michiganensis (Cm) causes bacterial canker of tomato. The infection process of Cm is of considerable interest, and the role of the alarmone guanosine tetraphosphate or pentaphosphate [(p)ppGpp], a global regulator,
Xiaoli Xu, Kaihong Bai, Jia Shi, Chengxuan Yu, Shuang Song, Na Jiang, Jianqiang Li, Laixin Luo   +7 more
doaj   +2 more sources