Effects of Guanosine Tetraphosphate, Guanosine Pentaphosphate, and β-γ Methylenyl-Guanosine Pentaphosphate on Gene Expression of Escherichia coli In Vitro [PDF]
Proceedings of the National Academy of Sciences, 1974 The effects of guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp), both produced by E. coli , were measured on the activities of several genes in a cell-free system. Gene activity is measured as gene-directed synthesis of biochemically competent protein or transfer RNA.
H L, Yang +4 more
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Guanosine tetraphosphate (ppGpp) signalling promotes high-light tolerance in Arabidopsis thaliana
bioRxivGuanosine tetraphosphate (ppGpp) is a hyperphosphorylated nucleotide originally discovered in prokaryotes and found in the chloroplasts of plants and algae.
Shanna Romand +12 more
semanticscholar +2 more sources
ppGpp Controls Global Gene Expression in Light and in Darkness in S. elongatus
Cell Reports, 2017 The bacterial and plant stringent response involves production of the signaling molecules guanosine tetraphosphate and guanosine pentaphosphate ((p)ppGpp), leading to global reorganization of gene expression.
Anna M. Puszynska, Erin K. O’Shea
doaj +2 more sources
Journal of Biological Chemistry, 1971 We have examined the intracellular levels of the guanosine tetraphosphate, guanosine 5'-diphosphate 2'- or 3'-diphosphate (ppGpp), in an isogenic pair of stringent and relaxed strains of Escherichia coli. As previously observed, relaxed strains do not accumulate ppGpp during amino acid deprivation, while stringent strains do.
R A, Lazzarini, M, Cashel, J, Gallant
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Molecular Microbiology, 2006 The RNA polymerase‐binding protein DksA is a cofactor required for guanosine tetraphosphate (ppGpp)‐responsive control of transcription from σ70 promoters.
Lisandro M. D. Bernardo +4 more
semanticscholar +2 more sources
Identification of the Synthesis of Guanosine Tetraphosphate (MS I) as Insertion of a Pyrophosphoryl Group into the 3′-Position in Guanosine 5′-Diphosphate [PDF]
Proceedings of the National Academy of Sciences, 1973 The phosphate transfer system of Haseltine et al. , consisting of a ribosomal wash obtained from a stringent strain of Escherichia coli , washed ribosomes, GTP, and ATP, was used to prepare large quantities of guanosine tetra- and pentaphosphates, the magic spot compounds MS I and MS II of ...
J, Sy, F, Lipmann
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Structural and mechanistic basis for the regulation of the chloroplast signal recognition particle by (p)ppGpp. [PDF]
FEBS LettLHCPs are transported to the thylakoid membrane via the (cp)SRP pathway. This process involves a transit complex of (cp)SRP43, (cp)SRP54 and LHCP, which interacts with (cp)FtsY and Alb3 at the membrane. GTP hydrolysis by (cp)SRP54 and (cp)FtsY triggers complex dissociation.
Zegarra V +7 more
europepmc +2 more sources
Maintenance of cytoplasmic and membrane densities shapes cellular geometry in Escherichia coli [PDF]
Nature CommunicationsMicrobes precisely control their composition and geometry across diverse growth conditions, yet the mechanisms coordinating these processes remain unclear.
Griffin Chure +4 more
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Loss of the plastidial signaling molecule, ppGpp, accumulation alters nuclear gene expression during nitrogen starvation [PDF]
Frontiers in Plant ScienceNitrogen (N) deficiency triggers major transcriptional reprogramming in plants. The chloroplast alarmone, guanosine tetraphosphate (ppGpp) synthesized and hydrolyzed by RelA-SpoT homologs (RSHs), has been proposed to regulate cellular metabolism.
Takanari Nemoto +10 more
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Wide occurrence of guanosine 5′-tetraphosphate in commercial preparations of GTP
Analytical Biochemistry, 1975 This investigation was supported by grants from the Fondo National para el Desarrollo de la Investigation Cientifica and the Jane Coffin Childs Memorial Fund for Medical Research. C.G.V. is a postdoctoral fellow of the Fundacidn Juan March.
Carmen G Vallejo +2 more
exaly +4 more sources