Results 261 to 270 of about 84,847 (305)

[37] Guanosine triphosphate-8-formylhydrolase

1975
Publisher Summary This chapter discusses the assay and purification procedure of guanosine triphosphate-8-formylhydrolase. The assay method is based on two procedures. The first method involves the release of 14 C-labeled formic acid from carbon-8 of the imidazole ring of [8- 14 C]GTP. The formic acid is oxidized to carbon dioxide. The second method
E.F. Elstner, R.J. Suhadolnik
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Adenosine Triphosphate and Guanosine Triphosphate Determinations in Intertidal Sediments

1979
Measurements were made of the spatial distributions of adenosine triphosphate (ATP) on a variety of sandy beaches. Samples were collected during ebb flow and extracted in the field using cold sulfuric acid and ethylenediamine tetraacetate (EDTA); the neutralized extracts were immediately frozen for subsequent analyses.
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The effect of guanosine 5′-triphosphate analogues on protein synthesis

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
Abstract Four GTP analogues, 6-mercaptoguanosine 5′-triphosphate (I), N2-methylguanosine 5′-triphosphate (II), N2-dimethylguanosine 5′-triphosphate (III) and 8-bromoguanosine 5′-triphosphate, (IV) were synthesized chemically and tested for their ability to replace GTP in the cell-free amino acid incorporation system of Escherichia coli.
H, Uno, S, Oyabu, E, Otsuka, M, Ikehara
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Direct incorporation of guanosine 5-diphosphate into microtubules without guanosine 5'-triphosphate hydrolysis

Biochemistry, 1986
Using highly purified calf brain tubulin bearing [8-14C]guanosine 5'-diphosphate (GDP) in the exchangeable nucleotide site and heat-treated microtubule-associated proteins (both components containing negligible amounts of nucleoside diphosphate kinase and nonspecific phosphatase activities), we have found that a significant proportion of exchangeable ...
E, Hamel, J K, Batra, C M, Lin
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Structural and Functional Relationships of Guanosine Triphosphate Binding Proteins

1988
Information available at present documents the existence of three well-defined classes of guanine nucleotide binding proteins functioning as signal transducers: Gs and Gi which stimulate and inhibit adenylate cyclase, respectively, and transducin which transmits and amplifies the signal from light-activated rhodopsin to cGMP-dependent phosphodiesterase
T, Pfeuffer, E J, Helmreich
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Preparative method of obtaining guanosine 5′-triphosphate

Chemistry of Natural Compounds, 1965
1. The kinetics of the formation of guanosine 5′-triphosphate, 5′-diphosphate, and 5′-monophosphate in the reaction of the morpholide of guanosine 5′-monophosphate with bis-(tributylammonium) pyrophosphate in pyridine at 27° has been recorded. 2. In the reaction of the morpholide of guanosine 5′-monophosphate with bis-(tributylammonium) pyrophosphate ...
T. S. Lomakina, N. I. Grineva
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Location of the Guanosine Triphosphate (GTP) Hydrolysis Site in Microtubulesa

Annals of the New York Academy of Sciences, 1986
The rate for GTP hydrolysis remains approximately constant during microtubule assembly from microtubular protein. This indicates that GTP hydrolysis does not accompany tubulin-GTP subunit addition to microtubule ends. We suggest that GTP, within tubulin-GTP subunits that are incorporated into microtubules, is hydrolyzed predominantly at one or both ...
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Neonatal hyperphenylalaninemia presumably caused by guanosine triphosphate-cyclohydrolase deficiency

The Journal of Pediatrics, 1985
C'est la recherche systematique des pteridines dans les urines qui a permis de suspecter le deficit en GTP-cyclohydrolase, 1ere etape de la synthese de la tetrahydrobiopterine (BH 4 ) chez un bebe hyperphenylalaninemique (HPA). Le deficit en BH 4 , variante rare de l'HPA, differe de la PKU classique par le caractere evolutif de l'atteinte neurologique ...
J L, Dhondt, J P, Farriaux, A, Boudha, C, Largillière, J, Ringel, M M, Roger, R J, Leeming   +6 more
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Guanosine-5?-triphosphate hydrolysis and tubulin polymerization

Molecular and Cellular Biochemistry, 1982
GTP hydrolysis associated with polymerization is a distinctive feature of microtubule assembly. This reaction may be fundamentally linked to the dynamic properties of microtubules in vivo. Kinetic analysis of the connection between microtubule assembly and associated GTP hydrolysis indicates that these two events are kinetically uncoupled, GTP ...
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Enzymatic synthesis of guanosine triphosphate from phytin and guanosine diphosphate

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1965
S, Biswas, B B, Biswas
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