CAP-MAP: cap analysis protocol with minimal analyte processing, a rapid and sensitive approach to analysing mRNA cap structures [PDF]
Open Biology, 2020 Eukaryotic messenger RNA (mRNA) is modified by the addition of an inverted guanosine cap to the 5′ triphosphate. The cap guanosine and initial transcribed nucleotides are further methylated by a series of cap methyltransferases to generate the mature cap
Alison Galloway +5 more
doaj +1 more source
Biophysica, 2023 We report the results of computational studies of the guanosine triphosphate (GTP) hydrolysis in the active site of the KRas-NF1 protein complex, where KRas stands for the K-isoform of the Ras (ras sarcoma) protein and NF1 (neurofbromin-1) is the ...
Igor Polyakov, Alexander Nemukhin
doaj +1 more source
The molecular genetics of RASopathies: An update on novel disease genes and new disorders
American Journal of Medical Genetics Part C: Seminars in Medical Genetics, Volume 190, Issue 4, Page 425-439, December 2022., 2022 Abstract Enhanced signaling through RAS and the mitogen‐associated protein kinase (MAPK) cascade underlies the RASopathies, a family of clinically related disorders affecting development and growth. In RASopathies, increased RAS‐MAPK signaling can result from the upregulated activity of various RAS GTPases, enhanced function of proteins positively ...
Marco Tartaglia +2 more
wiley +1 more source
Kinetics of Phosphate Incorporation into Adenosine Triphosphate and Guanosine Triphosphate in Bacteria [PDF]
Journal of Bacteriology, 1973 Escherichia coli cells were labeled with 33 PO 4 for several generations and then 32 PO 4 was added, samples were taken at various times, and adenosine triphosphate (ATP) and guanosine triphosphate (GTP) were purified from an acid ...
Michael W. Konrad +2 more
openaire +3 more sources
Regulation of the Single Polar Flagellar Biogenesis
Biomolecules, 2020 Some bacterial species, such as the marine bacterium Vibrio alginolyticus, have a single polar flagellum that allows it to swim in liquid environments.
Seiji Kojima +2 more
doaj +1 more source
Advanced Raman spectroscopy detection of oxidative damage in nucleic acid bases: probing chemical changes and intermolecular interactions in guanosine at ultralow concentration [PDF]
, 2021 DNA/RNA synthesis precursors are especially vulnerable to damage induced by reactive oxygen species occurring following oxidative stress. Guanosine triphosphates are the prevalent oxidized nucleosites, which can be misincorporated during replication, leading to mutations and cell death. Here, we present a novel method based on micro-Raman spectroscopy,
arxiv +1 more source
The roles of initiation factor 2 and guanosine triphosphate in initiation of protein synthesis
EMBO Journal, 2003 Ayman Antoun +4 more
openalex +2 more sources
Simultaneous quantification of 12 different nucleotides and nucleosides released from renal epithelium and in human urine samples using ion-pair reversed-phase HPLC [PDF]
, 2012 Nucleotides and nucleosides are not only involved in cellular metabolism but also act extracellularly via P1 and P2 receptors, to elicit a wide variety of physiological and pathophysiological responses through paracrine and autocrine signalling pathways.
Alberto Contreras-Sanz +60 more
core +2 more sources
Molecules, 2023 The therapeutic advantages of some platinum complexes as major anticancer chemotherapeutic agents and of nucleoside analogue-based compounds as essential antiviral/antitumor drugs are widely recognized.
Federica De Castro +10 more
doaj +1 more source
Guanosine triphosphate cyclohydrolase activity in rat tissues [PDF]
Biochemical Journal, 1984 The GTP cyclohydrolase activity of rat tissues has been studied by means of the measurement of formic acid release and neopterin synthesis from GTP. After gel filtration of a 45%-satd.-(NH4)2SO4 fraction of liver homogenates, three enzyme fractions were separated and named A1, A2 and A3 according to the order of their elution.
J L Dhondt, J P Farriaux, Z Bellahsene
openaire +3 more sources