Results 101 to 110 of about 323,472 (305)

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

FEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga, Toshiki Takahashi, Akiko Kuma, Hiroyuki Kawahara   +3 more
wiley   +1 more source

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source

Efficient gene editing of pig embryos by combining electroporation and lipofection [PDF]

Veterinary World
Background and Aim: Mosaicism, which is characterized by the presence of wild-type and more than one mutant allele, poses a serious problem in zygotic gene modification through the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR ...
Qingyi Lin, Nanaka Torigoe, Bin Liu, Yuichiro Nakayama, Aya Nakai, Zhao Namula, Megumi Nagahara, Fuminori Tanihara, Maki Hirata, Takeshige Otoi   +9 more
doaj   +1 more source

Epigenetic blind spots – the role of DNA methylation dynamics in stem cell‐based models of embryogenesis

FEBS Letters, EarlyView.
Embryo‐like structures (stembryos) are an innovative tool, but they are hindered by experimental variability and limited developmental potential. DNA methylation is crucial for mammalian development, but its status in stembryo models is poorly characterized.
Sara Canil, Anna Corrà, Annalisa Scapolatiello, Chengxiang Qiu, Gianluca Amadei   +4 more
wiley   +1 more source

Prokaryotic homologs of Argonaute proteins are predicted to function as key components of a novel system of defense against mobile genetic elements

, 2009
Background In eukaryotes, RNA interference (RNAi) is a major mechanism of defense against viruses and transposable elements as well of regulating translation of endogenous mRNAs.
Makarova, K.S., Koonin Eugene V, Kira S Makarova, Makarova Kira S, Koonin, E.V., Oost, J., van der, John van der Oost, van der Oost, J., Yuri I Wolf, van der Oost John, Wolf, Y.I., Wolf Yuri I, Eugene V Koonin   +12 more
core   +1 more source

Atlas der SARS-CoV-2-RNA-Protein-Interaktionen in infizierten Zellen

, 2021
Using RNA antisense purification and mass spectrometry, we identified more than 100 human proteins that directly and specifically bind SARS-CoV-2 RNA in infected cells.
Munschauer, Mathias, Schmidt, Nora
core   +1 more source

Structural insights into C-terminus-mediated RNA target cleavage by a mesophilic prokaryotic argonaute

Nature Communications
Prokaryotic Argonaute proteins (pAgos) are programmable nucleases that always utilize DNA guides to cleave DNA targets. Recent studies show that some pAgos preferentially utilize DNA guides to cleave RNA targets rather than DNA targets.
Taiyu Chen, Xin Tao, Shunshun Li, Qingmiao Duanmu, Jiening Wang, Kai Chen, Kangle Mu, Hong Yang, Yu Li, Yang Liu, Lixin Ma, Shan Wu   +11 more
doaj   +1 more source

Conversion of IscB and Cas9 into RNA-guided RNA editors

Cell
RNA-guided RNA editing represents an attractive alternative to DNA editing. However, the prevailing tool, CRISPR-Cas13, has collateral RNA cleavage activity that causes undesirable cytotoxicity in human cells. Here, we report an ultracompact RNA-editing platform engineered from IscB, which has comparable or higher activity than Cas13 but without ...
Xu, Chengtao, Niu, Xiaolin, Sun, Haifeng, Yan, Hao, Tang, Weixin, Ke, Ailong   +5 more
openaire   +2 more sources

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source

RNA editing in kinetoplastids

, 2010
RNA editing in kinetoplastid protozoa is a post-transcriptional process of uridine insertion or deletion in mitochondrial mRNAs. The process involves two RNA species, the pre-edited mRNA and in most cases a trans-acting guide RNA (gRNA). Sequences within
Hajduk, Stephen, Torsten Ochsenreiter, Stephen Hajduk, Ochsenreiter, Torsten   +3 more
core   +1 more source