Results 101 to 110 of about 2,639,376 (307)

Improved annotation of 3' untranslated regions and complex loci by combination of strand-specific direct RNA sequencing, RNA-seq and ESTs [PDF]

, 2014
The reference annotations made for a genome sequence provide the framework for all subsequent analyses of the genome. Correct and complete annotation in addition to the underlying genomic sequence is particularly important when interpreting the results ...
Junfang Song, Geoffrey J Barton, Schurch Nicholas J., Brown, Sara J., Gordon G Simpson, Nicholas J Schurch, McLean, W. H. Irwin, Song Junfang, Simpson Gordon G., Sherstnev, Alexander, Brown Sara J., Alexander Sherstnev, Song, Junfang, McLean W. H. Irwin, Duc, Celine, Kate G Storey, Storey, Kate G., Schurch, Nicholas J., Cole Christian, Sherstnev Alexander, Simpson, Gordon G., Céline Duc, W H Irwin McLean, Cole, Christian; id_orcid, Christian Cole, Barton Geoffrey J., Sara J Brown, Duc Céline, Barton, Geoffrey J.; id_orcid, Storey Kate G.   +29 more
core   +1 more source

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

FEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil, Jeffrey A. Hawkes, Cecilia Blikstad, Pia Lindberg   +3 more
wiley   +1 more source

A population study of the minicircles in Trypanosoma cruzi: predicting guide RNAs in the absence of empirical RNA editing

BMC Genomics, 2007
Background The structurally complex network of minicircles and maxicircles comprising the mitochondrial DNA of kinetoplastids mirrors the complexity of the RNA editing process that is required for faithful expression of encrypted maxicircle genes ...
Westenberger Scott J, Martinez LL Isadora, Thomas Sean, Sturm Nancy R   +3 more
doaj   +1 more source

A viral guide RNA delivery system for CRISPR-based transcriptional activation and heritable targeted DNA demethylation in Arabidopsis thaliana.

PLoS Genetics, 2020
Plant RNA viruses are used as delivery vectors for their high level of accumulation and efficient spread during virus multiplication and movement. Utilizing this concept, several viral-based guide RNA delivery platforms for CRISPR-Cas9 genome editing ...
Basudev Ghoshal, Brandon Vong, Colette L Picard, Suhua Feng, Janet M Tam, Steven E Jacobsen   +5 more
doaj   +1 more source

RNA‐related functions of BRCA2 in transcription, replication, and genome stability

FEBS Letters, EarlyView.
Beyond its role in homologous recombination, BRCA2 regulates R‐loop homeostasis, DNA–RNA hybrid resolution, RNA polymerase II dynamics, and transcriptional activation. Disruption of any of these functions can contribute to genome instability, inflammatory signaling, and tumorigenesis. BRCA2 is a central tumor suppressor protein best known for its roles
Lucia Alvaro‐Aranda, Sreerama Chaitanya Sridhara, Aura Carreira   +2 more
wiley   +1 more source

A splicing-dependent transcriptional checkpoint associated with prespliceosome formation [PDF]

, 2014
There is good evidence for functional interactions between splicing and transcription in eukaryotes, but how and why these processes are coupled remain unknown.
Chathoth, Keerthi T, Webb, Shaun, Barrass, J David, Beggs, Jean D   +3 more
core   +1 more source

A context‐dependent modulatory role for eIF6 in acquired resistance to vemurafenib in melanoma

FEBS Letters, EarlyView.
Acquired resistance to vemurafenib upregulates the translation factor eIF6 in melanoma cells. Silencing eIF6 in resistant cells reduces proliferation and partially restores drug sensitivity, whereas its overexpression increases sensitivity across melanoma lines regardless of BRAF status, via modulation of mTOR, S6K, and MAPK signaling.
George Kyriakopoulos, Antonia Petropoulou, Alexandra Anastogianni, Angelina Bania, Vassilis Stamatakis, Argyris Alexiou, Chi‐Ping Day, Glenn Merlino, Katerina Grafanaki, Constantinos Stathopoulos   +9 more
wiley   +1 more source

The NS3 protein of rice hoja blanca virus suppresses RNA silencing in mammalian cells [PDF]

, 2008
The NS3 protein of the tenuivirus rice hoja blanca virus (RHBV) has previously been shown to represent the viral RNA interference (RNAi) suppressor and is active in both plant and insect cells by binding short interfering RNAs (siRNAs) in vitro.
Hemmes, J.C., Schnettler, E., Goldbach, R.W., Prins, M.W.   +3 more
core   +1 more source

Membrane composition and thermodynamic identity as boundaries of life for synthetic cell research

FEBS Letters, EarlyView.
What makes a cell a cell? The boundary of a living cell is not just a wall. Read as a Markov blanket, the membrane separates internal from external states, generating identity and non‐equilibrium order. Can this identity be rebuilt from scratch in a synthetic cell?
Caterina Presutti, Bert Poolman
wiley   +1 more source

Establishing single cell RNA transcriptomics: a brief guide

Frontiers in Zoology
Single cell RNA sequencing is a tool for evaluating the specific transcriptome usage of different cell types within an organism. By tagging mRNA molecules from single cells or nuclei, a non-biased assay of the active transcriptome is captured. The method
Alison G. Cole
doaj   +1 more source